Molecular assessment of differences in the duodenal microbiome in subjects with irritable bowel syndrome

• Published in: Scandinavian journal of gastroenterology Vol. 50; no. 9; p. 1076
• Main Authors: Giamarellos-Bourboulis, Evangelos, Tang, Jie, Pyleris, Emmannouil, Pistiki, Aikaterini, Barbatzas, Charalambos, Brown, Jordan, Lee, Clarence C, Harkins, Timothy T, Kim, Gene, Weitsman, Stacy, Barlow, Gillian M, Funari, Vincent A, Pimentel, Mark
• Format: Journal Article
• Language: English
• Published: England 01.01.2015
• Subjects: Adult; Aged; Aged, 80 and over; Case-Control Studies; DNA, Bacterial - analysis; DNA, Bacterial - isolation & purification; Duodenum - microbiology; Endoscopy, Gastrointestinal; Feces - microbiology; Female; Gastrointestinal Microbiome - genetics; Humans; Irritable Bowel Syndrome - microbiology; Male; Middle Aged; Prospective Studies; Real-Time Polymerase Chain Reaction; small intestinal bacterial overgrowth; irritable bowel syndrome; quantitative PCR; next generation sequencing; duodenum

Breath testing and duodenal culture studies suggest that a significant proportion of irritable bowel syndrome (IBS) patients have small intestinal bacterial overgrowth. In this study, we extended these data through 16S rDNA amplicon sequencing and quantitative PCR (qPCR) analyses of duodenal aspirat...