Validation of a multiplexed LC–MS/MS clinical assay to quantify insulin-like growth factor-binding proteins in human serum and its application in a clinical study

• Published in: Toxicology and applied pharmacology Vol. 371; pp. 74 - 83
• Main Authors: Liu, Nanjun, Tengstrand, Elizabeth, Boernsen, K. Olaf, Bek, Stephan, Hsieh, Frank
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.05.2019
• Subjects: Alkylation reagent; Biomarkers - blood; Bulbo-Spinal Atrophy, X-Linked - blood; Bulbo-Spinal Atrophy, X-Linked - diagnosis; Calibration; Chromatography, Liquid - standards; Clinical Trials, Phase II as Topic; Humans; Insulin-Like Growth Factor Binding Proteins - blood; Insulin-like growth factor-binding proteins; LC–MS/MS; Male; Matrix effect; Predictive Value of Tests; Protein quantitation; Reference Standards; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization - standards; Spinal and bulbar muscular atrophy; Tandem Mass Spectrometry - standards; Protein quantitation; Spinal and bulbar muscular atrophy; Matrix effect; Alkylation reagent; Insulin-like growth factor-binding proteins; LC–MS/MS

Circulating insulin-like growth factor-binding proteins (IGFBPs) continue to gain attention as biomarkers of drug activities on insulin like growth factor (IGF)/IGF receptor signaling pathways. A multiplexed LC–MS/MS method was validated for the absolute quantitation of IGFBPs in human serum. The me...