Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes

• Published in: Biodegradation (Dordrecht) Vol. 18; no. 1; pp. 115 - 121
• Main Authors: ZULFIQAR ALI RAZA, REHMAN, Asma, MUHAMMAD SALEEM KHAN, KHALID, Zafar M
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.02.2007; Springer Nature B.V
• Subjects: Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biomass; Biotechnology; Canola Oil; Environment and pollution; Fatty Acids, Monounsaturated - metabolism; Fundamental and applied biological sciences. Psychology; Glycolipids - metabolism; Industrial applications and implications. Economical aspects; Industrial Waste - analysis; Mutation; Nitrates - metabolism; Petroleum refineries; Plant Oils - metabolism; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - metabolism; Surface Tension; Surface-Active Agents - metabolism; Pseudomonadales; Biodegradation; Vegetable oil; Refinery; vegetable oil wastes; Biosurfactant; Emulsion; Production; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Yield; Wastes; Mutation; yield factors
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-006-9047-9

Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l(-1)) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l(-1)) and rhamnolipid production (8.50 g l(-1)), at 10 d of incubation with the specific growth rate of 0.316 h(-1) and specific product yield of 0.597 g g(-1) h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m(-1).