Three-dimensional microchannel reflecting cell size distribution for on-chip production of platelet-like particles

• Published in: Microfluidics and nanofluidics Vol. 25; no. 4
• Main Authors: Sakuma, Shinya, Kumon, Hiroki, Nakamura, Sou, Kasai, Yusuke, Eto, Koji, Arai, Fumihito
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2021; Springer Nature B.V
• Subjects: Analytical Chemistry; Biomedical Engineering and Bioengineering; Bioreactors; Blood & organ donations; Blood platelets; Cell lines; Cell size; Cross-sections; Design; Engineering; Engineering Fluid Dynamics; Flow rates; Flow velocity; Human influences; Man-induced effects; Megakaryocytes; Microchannels; Microfluidics; Nanotechnology and Microengineering; Particle size distribution; Platelets; Pluripotency; Research Paper; Size distribution; Stem cells; Trapping; Fabrication; Microfluidic chip; 3D microchannel; Bioreactor; Platelet

Recently, microfluidic bioreactors that trap injected megakaryocytes (MKs) by application of fluid force to them have been proposed as small test benches to evaluate the in vitro platelet production process. However, making a flow rate constant after trapping MKs remains a challenge and bottleneck b...