Chromatin Accessibility Dynamics during iPSC Reprogramming

• Published in: Cell stem cell Vol. 21; no. 6; pp. 819 - 833.e6
• Main Authors: Li, Dongwei, Liu, Jing, Yang, Xuejie, Zhou, Chunhua, Guo, Jing, Wu, Chuman, Qin, Yue, Guo, Lin, He, Jiangping, Yu, Shenyong, Liu, He, Wang, Xiaoshan, Wu, Fang, Kuang, Junqi, Hutchins, Andrew P., Chen, Jiekai, Pei, Duanqing
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.12.2017
• Subjects: Animals; binary logic; Cells, Cultured; Cellular Reprogramming; Chromatin - genetics; Chromatin - metabolism; chromatin dynamics; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Kruppel-Like Transcription Factors - metabolism; Mice; Octamer Transcription Factor-3 - metabolism; open/close; reprogramming; Sap30; SOXB1 Transcription Factors - metabolism; reprogramming; Sap30; chromatin dynamics; binary logic; open/close

Cell-fate decisions remain poorly understood at the chromatin level. Here, we map chromatin remodeling dynamics during induction of pluripotent stem cells. ATAC-seq profiling of MEFs expressing Oct4-Sox2-Klf4 (OSK) reveals dynamic changes in chromatin states shifting from open to closed (OC) and clo...