Rapid analytical method for the determination of organic and inorganic species in tomato samples through HPLC–ICP-AES coupling

• Published in: Food chemistry Vol. 111; no. 2; pp. 469 - 475
• Main Authors: Paredes, E., Prats, M.S., Maestre, S.E., Todolí, J.L.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 15.11.2008; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: Biological and medical sciences; Carbon; Carboxylic acids; cultivars; food analysis; food composition; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; High performance liquid chromatography; Inductively coupled plasma atomic emission spectrometry; ions; Metals; organic compounds; organic foods; Phosphates; rapid methods; refractive index; Sugars; Sulphates; Tomato; tomatoes; Phosphates; Sulphates; Metals; Carboxylic acids; Tomato; Inductively coupled plasma atomic emission spectrometry; Carbon; High performance liquid chromatography; Sugars; Vegetables; Sample; HPLC chromatography; Metal; Method; Emission spectrometry; Carboxylic acid; High performance liquid chromatography,Inductively coupled plasma atomic emission,spectrometry,Carbon,Sugars,Carboxylic acids,Metals,Sulphates,Phosphates,Tomato; Species; Sugar
• DOI: 10.1016/j.foodchem.2008.03.083
• ISSN: 0308-8146

A HPLC–inductively coupled plasma atomic emission spectrometer (ICP-AES) hyphenation technique was used to determine the concentration of some organic (i.e., carbohydrates, carboxylic acids) as well as inorganic (metals and anions) compounds in tomato samples. A high efficiency nebulizer (HEN) coupled to a low inner volume cyclonic spray chamber (Cinnabar) was used to interface both techniques. The HPLC–ICP-AES chromatograms for organic compounds were obtained by plotting the 193.03nm carbon emission intensity versus time. In the present work, it was also possible to obtain information about the concentration of several metals in foodstuffs. Finally, by registering the intensity at the sulphur and phosphorous emission wavelengths, the content of anions such as sulphate and phosphate was determined. In general terms, the results obtained with HPLC–ICP-AES did not differ significantly from those found with a refractive index detector. Due to the huge amount of information provided by this hyphenation, it was possible to apply it to the discrimination among different samples of native tomato cultivars.