The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L

• Published in: Gene Vol. 776; p. 145443
• Main Authors: Katsuyama, Yushi, Doi, Mizuho, Shioya, Sachi, Hane, Sanae, Yoshioka, Momoko, Date, Shuichi, Miyahara, Chika, Ogawa, Tomomichi, Takada, Ryo, Okumura, Hanako, Ikusawa, Rie, Kitajima, Sakihito, Oda, Kenji, Sato, Kenji, Tanaka, Yoshikazu, Tezuka, Takahiro, Mino, Masanobu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2021
• Subjects: Carrier Proteins - genetics; Cell Death - genetics; Chaperone complex; Cytoplasm - metabolism; Gene Expression - genetics; Gene Expression Profiling - methods; Gene Expression Regulation, Plant - genetics; Genotype; HSP90 Heat-Shock Proteins - genetics; Hybrid inviability; Hybrid Vigor - genetics; Hydrogen Peroxide - metabolism; Intracellular Signaling Peptides and Proteins - genetics; Molecular Chaperones - genetics; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana spp; NLR proteins; Plant Proteins - genetics; RAR1; Seedlings - genetics; SGT1; Transcriptome - genetics; Chaperone complex; CD; HSP90; Hybrid inviability; NB-LRR; Nicotiana spp; NLR proteins; DMSO; RAR1; CCH; Y2H; CS; PCD; CHORD1 and 2; ND; SGT1; MD; NLR proteins, nucleotide-binding domain; TPR; SGS
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2021.145443

•Chaperone complexes may be involved in the hybrid inviabilty in Nicotiana.•SGT1 and RAR1 of N. gossei acted as cell death inducers in the leaves of N. tabacum.•RAR1 in the complex was a major regulator of the cell death reaction in N. tabacum.•Inhibition of HSP90 suppressed cell death of F1 hybrids and death induced by RAR1.•Autoimmune response in hybrid inviability may not solely depend on NLR proteins. Two cultured cell lines (GTH4 and GTH4S) of a Nicotiana interspecific F1 hybrid (N. gossei × N. tabacum) were comparatively analyzed to find genetic factors related to hybrid inviability. Both cell lines proliferated at 37 °C, but after shifting to 26 °C, GTH4 started to die similar to the F1 hybrid seedlings, whereas GTH4S survived. As cell death requires de novo expression of genes and proteins, we compared expressed protein profiles between the two cell lines, and found that NgSGT1, a cochaperone of the chaperone complex (HSP90-SGT1-RAR1), was expressed in GTH4 but not in GTH4S. Agrobacterium-mediated transient expression of NgSGT1, but not NtSGT1, induced cell death in leaves of N. tabacum, suggesting its possible role in hybrid inviability. Cell death in N. tabacum was also induced by transient expression of NgRAR1, but not NtRAR1. In contrast, transient expression of any parental combinations of three components revealed that NgRAR1 promoted cell death, whereas NtRAR1 suppressed it in N. tabacum. A specific inhibitor of HSP90, geldanamycin, inhibited the progression of hypersensitive response-like cell death in GTH4 and leaf tissue after agroinfiltration. The present study suggested that components of the chaperone complex are involved in the inviability of Nicotiana interspecific hybrid.