Oncogene-like addiction to aneuploidy in human cancers

• Published in: Science (American Association for the Advancement of Science) Vol. 381; no. 6660; p. eadg4521
• Main Authors: Girish, Vishruth, Lakhani, Asad A., Thompson, Sarah L., Scaduto, Christine M., Brown, Leanne M., Hagenson, Ryan A., Sausville, Erin L., Mendelson, Brianna E., Kandikuppa, Pranav K., Lukow, Devon A., Yuan, Monet Lou, Stevens, Eric C., Lee, Sophia N., Schukken, Klaske M., Akalu, Saron M., Vasudevan, Anand, Zou, Charles, Salovska, Barbora, Li, Wenxue, Smith, Joan C., Taylor, Alison M., Martienssen, Robert A., Liu, Yansheng, Sun, Ruping, Sheltzer, Jason M.
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 25.08.2023
• Subjects: Addictions; Aneuploidy; Anticancer properties; Antitumor agents; Biocompatibility; Cancer; Carcinogenesis - genetics; Cell Cycle Proteins - genetics; Cell survival; Chromosome 1; Chromosomes; Copy number; CRISPR; Cytotoxicity; Dosage; Engineering; Exhibits; Gene Editing - methods; Gene expression; Genomes; Humans; In vivo methods and tests; Karyotypes; Kinases; Malignancy; Mutation; Neoplasms - genetics; Neoplasms - therapy; Nucleotide analogs; Nucleotides; Oncogenes; p53 Protein; Proto-Oncogene Proteins - metabolism; Sensitivity enhancement; Substance Abuse; Toxicity; Trisomy; Tumor cell lines; Tumor suppressor genes; Tumor Suppressor Protein p53 - genetics; Tumorigenesis; Tumors; Xenotransplantation

Most cancers exhibit aneuploidy, but its functional significance in tumor development is controversial. Here, we describe ReDACT (Restoring Disomy in Aneuploid cells using CRISPR Targeting), a set of chromosome engineering tools that allow us to eliminate specific aneuploidies from cancer genomes. U...