Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method

• Published in: Journal of virological methods Vol. 92; no. 1; pp. 19 - 22
• Main Authors: Vilček, Štefan, Strojny, Ladislav, Ďurkovič, Branislav, Rossmanith, Wigbert, Paton, David
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.03.2001; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Bovine viral diarrhea virus; Bovine viral diarrhoea virus; Bovine Virus Diarrhea-Mucosal Disease - virology; BVDV; Cattle; Detection; Diarrhea Viruses, Bovine Viral - genetics; Diarrhea Viruses, Bovine Viral - isolation & purification; Filter; Fundamental and applied biological sciences. Psychology; Microbiology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - analysis; RT-PCR; Specimen Handling - methods; Specimen Handling - veterinary; BVDV; Bovine viral diarrhoea virus; Detection; Filter; RT-PCR; Pestivirus; Typing; Bovine; Diarrhea; Blood; Virus; Polymerase chain reaction; Vertebrata; Mammalia; Storage; Infectivity; Bovine diarrhea virus; Digestive diseases; Intestinal disease; Serum; Artiodactyla; Diagnosis; Flaviviridae; Ungulata

Of four solid carriers tested, Whatman paper No 1 was the best for storing blood and serum samples for the diagnosis of bovine viral diarrhoea (BVD) by means of viral RNA detection. The filter papers were impregnated with 10 μl of blood or serum, followed by air drying. Samples collected in this way...