UPLC–MS/MS method for the simultaneous quantification of three new antiretroviral drugs, dolutegravir, elvitegravir and rilpivirine, and other thirteen antiretroviral agents plus cobicistat and ritonavir boosters in human plasma

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 138; pp. 223 - 230
• Main Authors: Simiele, Marco, Ariaudo, Alessandra, De Nicolò, Amedeo, Favata, Fabio, Ferrante, Martina, Carcieri, Chiara, Bonora, Stefano, Di Perri, Giovanni, D’Avolio, Antonio
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 10.05.2017
• Subjects: Anti-HIV Agents - blood; Anti-HIV Agents - chemistry; Anti-Retroviral Agents - blood; Anti-Retroviral Agents - chemistry; Antiretrovirals; Chromatography, High Pressure Liquid - methods; Cobicistat - blood; Cobicistat - chemistry; HAART; Heterocyclic Compounds, 3-Ring - blood; Heterocyclic Compounds, 3-Ring - chemistry; Humans; Limit of Detection; Liquid chromatography; Protein precipitation; Quinolones - blood; Quinolones - chemistry; Reproducibility of Results; Reverse phase; Rilpivirine - blood; Rilpivirine - chemistry; Ritonavir - blood; Ritonavir - chemistry; Tandem mass spectrometry; Tandem Mass Spectrometry - methods; Tandem mass spectrometry; Antiretrovirals; Liquid chromatography; Reverse phase; Protein precipitation; HAART

Typical chromatogram from direct injection of a chemical mix containing all the target analytes at a concentration of 2μg/mL. [Display omitted] •Useful UHPLC–MS/MS method to quantify a wide panel of antiretroviral drugs.•Validated method following FDA guidelines.•Fast and cheap protein precipitation...