Isolation and partial characterization of two antifungal proteins from barley

• Published in: Biochimica et biophysica acta Vol. 880; no. 2; pp. 161 - 170
• Main Authors: Roberts, Walden K., Selitrennikoff, Claude P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.02.1986; Elsevier; North-Holland
• Subjects: Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal agents; Antifungal Agents - isolation & purification; Antifungal protein; Barley; Biological and medical sciences; characterization; Chromatography, Gel; Defense mechanism; Edible Grain - analysis; Electrophoresis, Polyacrylamide Gel; Fungi - drug effects; Fungi - growth & development; Hordeum - analysis; Hordeum vulgare; isolation; Medical sciences; Microbial Sensitivity Tests; Molecular Weight; Pharmacology. Drug treatments; Plant Proteins - isolation & purification; Plant Proteins - pharmacology; proteins; Ribosome-inactivating protein; Temperature; Barley; Defense mechanism; Ribosome-inactivating protein; Antifungal protein; Microorganism growth; Ribosome; Phycomycetes; Alternaria; Fungistasis; Phycomyces blakesleeanus; Host agent relation; Trichoderma reesei; Biological activity; Fungicide; Characterization; Fungi; Proteins; Plant origin; Ascomycetes; Neurospora crassa; Fungi Imperfecti; Inhibition; Mechanism of action; Thallophyta

We have developed a simple assay for detecting antifungal compounds utilizing impregnated paper discs on agar to inhibit mycelial spread of an indicator organism, richoderma reesei isolated and purified to apparent homogeneity two antifungal proteins from dehusked barley grain. Both proteins are pre...