Liquid chromatography–tandem mass spectrometry analysis of nitazoxanide and its major metabolites in goat

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 875; no. 2; pp. 427 - 436
• Main Authors: Zhao, Zhanzhong, Zhang, Lifang, Xue, Feiqun, Wang, Xiaoyang, Zheng, Wenli, Zhang, Tao, Fei, Chenzhong, Zhang, Keyu, Qiu, Minqi, Xin, Ruixiang, Yang, Fengkun
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.11.2008; Elsevier
• Subjects: Administration, Oral; Analysis; Analytical, structural and metabolic biochemistry; Animals; Antiparasitic Agents - administration & dosage; Antiparasitic Agents - blood; Antiparasitic Agents - metabolism; Antiparasitic Agents - urine; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Goat; Goats - metabolism; LC-MS-MS; Male; Medical sciences; Metabolites; Nitazoxanide; Pharmacology. Drug treatments; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; Thiazoles - administration & dosage; Thiazoles - blood; Thiazoles - metabolism; Thiazoles - urine; LC–MS–MS; Goat; Nitazoxanide; Metabolites; Anthelmintic; Metabolite; HPLC chromatography; Prodrug; Antiprotozoal agent; Vertebrata; Mammalia; Analysis; Mass spectrometry MS/MS; Parasiticide; Artiodactyla; Ungulata; LC-MS-MS

A rapid, sensitive and specific liquid chromatography–electrospray ionization (ESI) tandem mass spectrometry (LC–MS–MS) method has been developed for the identification of nitazoxanide metabolites in goat plasma and urine. The purified samples was separated using an XTerra MS C8 column with the mobi...