Comparative evaluation of three anti-dsDNA antibody detection methods in systemic lupus erythematosus: insights from a large monocentric cohort

• Published in: Frontiers in immunology Vol. 16; p. 1529484
• Main Authors: Lu, Ruijing, Yu, Rui, Huang, Rong, Xue, Chiyuan, Song, Ning, Zhao, Jiuliang, Zeng, Xiaofeng, Hu, Chaojun
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 10.04.2025
• Subjects: Adult; anti-double-stranded DNA; Antibodies, Antinuclear - blood; Antibodies, Antinuclear - immunology; chemiluminescence immunoassay; digital liquid chip method; Female; Fluorescent Antibody Technique, Indirect - methods; Humans; Immunology; indirect immunofluorescence assay; Luminescent Measurements - methods; Lupus Erythematosus, Systemic - blood; Lupus Erythematosus, Systemic - diagnosis; Lupus Erythematosus, Systemic - immunology; Male; Middle Aged; Predictive Value of Tests; Retrospective Studies; Sensitivity and Specificity; systemic lupus erythematosus; anti-double-stranded DNA; systemic lupus erythematosus; digital liquid chip method; chemiluminescence immunoassay; indirect immunofluorescence assay

Anti-double-stranded DNA (anti-dsDNA) antibodies at abnormal titer are of considerable diagnostic value for systemic lupus erythematosus (SLE). Current assays detecting anti-dsDNA antibodies show divergent properties, emphasizing the importance of selecting suitable assays. This study aims to invest...