Membrane potential drives direct translocation of cell-penetrating peptides
Cell-penetrating peptides (CPPs) are frequently employed as drug delivery agents with rapid cellular uptake, however, the uptake mechanism and the detailed translocation pathway are at present not completely understood. Both endocytosis and direct translocation through membrane pores have been obser...
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Published in | Nanoscale Vol. 11; no. 4; pp. 1949 - 1958 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
23.01.2019
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Abstract | Cell-penetrating peptides (CPPs) are frequently employed as drug delivery agents with rapid cellular uptake, however, the uptake mechanism and the detailed translocation pathway are at present not completely understood. Both endocytosis and direct translocation through membrane pores have been observed in experiments and simulations under different conditions. Here we report the molecular dynamics simulations providing evidence for the direct translocation of CPPs across the membrane driven by the membrane electrostatic potential. The local membrane potential can be produced by the ion concentration imbalance across the membrane, which is ubiquitous in biological environments. Moreover, if positively charged CPPs are adsorbed on the membrane, this further enhances the membrane potential, opening membrane pores through which CPPs can be instantly transported in a chain-like configuration. The classical nucleation theory is applied to estimate the translocation time by calculating the changes in the free energy upon transferring CPPs across the membrane at different potentials, showing good agreement with available experimental measurements. The revealed CPP translocation mechanism can be broadly relevant for cellular processes in biology. |
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AbstractList | Cell-penetrating peptides (CPPs) are frequently employed as drug delivery agents with rapid cellular uptake, however, the uptake mechanism and the detailed translocation pathway are at present not completely understood. Both endocytosis and direct translocation through membrane pores have been observed in experiments and simulations under different conditions. Here we report the molecular dynamics simulations providing evidence for the direct translocation of CPPs across the membrane driven by the membrane electrostatic potential. The local membrane potential can be produced by the ion concentration imbalance across the membrane, which is ubiquitous in biological environments. Moreover, if positively charged CPPs are adsorbed on the membrane, this further enhances the membrane potential, opening membrane pores through which CPPs can be instantly transported in a chain-like configuration. The classical nucleation theory is applied to estimate the translocation time by calculating the changes in the free energy upon transferring CPPs across the membrane at different potentials, showing good agreement with available experimental measurements. The revealed CPP translocation mechanism can be broadly relevant for cellular processes in biology. |
Author | Dobnikar, Jure Liu, Zhiping Gao, Xinli Zhang, Xianren Hong, Song Yue, Tongtao |
Author_xml | – sequence: 1 givenname: Xinli surname: Gao fullname: Gao, Xinli organization: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China – sequence: 2 givenname: Song surname: Hong fullname: Hong, Song – sequence: 3 givenname: Zhiping surname: Liu fullname: Liu, Zhiping – sequence: 4 givenname: Tongtao surname: Yue fullname: Yue, Tongtao – sequence: 5 givenname: Jure surname: Dobnikar fullname: Dobnikar, Jure – sequence: 6 givenname: Xianren surname: Zhang fullname: Zhang, Xianren |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30644958$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1021/bi501392n 10.1021/jp107763b 10.1074/jbc.M303045200 10.1016/j.biocel.2012.02.010 10.1093/bioinformatics/btt055 10.1016/j.bbamem.2010.09.009 10.1021/ja053129n 10.1038/nm996 10.1021/acsnano.6b07563 10.1074/jbc.M209548200 10.1021/jp310255r 10.1021/nl049170q 10.1186/1471-2091-5-10 10.1038/ncomms1459 10.1016/S0001-8686(00)00061-0 10.1016/j.bpj.2012.10.027 10.1021/nn4040553 10.1021/ja507790z 10.1016/j.bioelechem.2014.03.009 10.1063/1.470117 10.1073/pnas.1108795108 10.1016/S1359-6446(04)03042-9 10.1016/j.jconrel.2017.04.013 10.1063/1.2217737 10.1021/jp412600e 10.1063/1.464397 10.1039/C2SM26519B 10.1073/pnas.1411817111 10.1074/jbc.272.25.16010 10.1016/j.jconrel.2011.03.011 10.1016/j.bpj.2009.05.066 10.1016/j.addr.2004.10.010 10.1016/j.bbcan.2008.03.001 10.1529/biophysj.104.050617 10.1529/biophysj.108.129437 10.1073/pnas.0435906100 10.1021/ja0482536 10.1021/ja029504i 10.1063/1.1826056 10.1021/bi035293y 10.1021/acsnano.5b07036 10.1007/s00232-010-9277-y 10.1063/1.2052648 10.1073/pnas.0706574105 10.1038/260799a0 10.2174/1389203033487298 10.1002/jcc.20090 10.1021/nl3029637 10.1146/annurev.bb.18.060189.000553 10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H 10.1021/jp3095165 10.1016/j.bpj.2010.03.065 10.1002/jcc.540130812 10.1016/j.bpj.2008.11.073 10.1529/biophysj.104.052787 10.1038/ncomms12906 10.1016/S1525-0016(03)00122-9 10.1063/1.2060666 10.1016/j.bpj.2009.03.059 10.1039/C4CP02211D 10.1111/j.1600-0854.2007.00572.x 10.1517/17425247.5.1.105 10.1007/s00232-012-9434-6 10.1016/S0006-3495(97)78845-3 |
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References | Tarek (C8NR10447F-(cit47)/*[position()=1]) 2005; 88 Casciola (C8NR10447F-(cit63)/*[position()=1]) 2014; 100 Foged (C8NR10447F-(cit3)/*[position()=1]) 2007; 5 Gurtovenko (C8NR10447F-(cit42)/*[position()=1]) 2005; 127 Tieleman (C8NR10447F-(cit51)/*[position()=1]) 2003; 125 Duchardt (C8NR10447F-(cit1)/*[position()=1]) 2007; 8 Chen (C8NR10447F-(cit10)/*[position()=1]) 2012; 4 Hu (C8NR10447F-(cit34)/*[position()=1]) 2014; 118 Levine (C8NR10447F-(cit46)/*[position()=1]) 2010; 236 Mishra (C8NR10447F-(cit15)/*[position()=1]) 2011; 108 Huang (C8NR10447F-(cit29)/*[position()=1]) 2013; 104 Hess (C8NR10447F-(cit59)/*[position()=1]) 1997; 18 Vivès (C8NR10447F-(cit18)/*[position()=1]) 1997; 272 Herce (C8NR10447F-(cit12)/*[position()=1]) 2014; 136 Grafmüller (C8NR10447F-(cit66)/*[position()=1]) 2009; 96 Maniti (C8NR10447F-(cit14)/*[position()=1]) 2012; 44 Yesylevskyy (C8NR10447F-(cit37)/*[position()=1]) 2009; 97 Sun (C8NR10447F-(cit30)/*[position()=1]) 2014; 16 Fittipaldi (C8NR10447F-(cit20)/*[position()=1]) 2004; 278 Clarke (C8NR10447F-(cit40)/*[position()=1]) 2001; 89 Terrone (C8NR10447F-(cit17)/*[position()=1]) 2003; 42 Lensink (C8NR10447F-(cit31)/*[position()=1]) 2005; 88 Li (C8NR10447F-(cit53)/*[position()=1]) 2012; 9 Pourmousa (C8NR10447F-(cit36)/*[position()=1]) 2013; 117 Neher (C8NR10447F-(cit38)/*[position()=1]) 1976; 260 Pronk (C8NR10447F-(cit54)/*[position()=1]) 2013; 29 Ferrari (C8NR10447F-(cit19)/*[position()=1]) 2003; 8 Rothbard (C8NR10447F-(cit16)/*[position()=1]) 2004; 126 Li (C8NR10447F-(cit60)/*[position()=1]) 2016; 7 Yesylevskyy (C8NR10447F-(cit24)/*[position()=1]) 2009; 97 Kästner (C8NR10447F-(cit61)/*[position()=1]) 2005; 123 Mao (C8NR10447F-(cit33)/*[position()=1]) 2016; 10 Rossi (C8NR10447F-(cit5)/*[position()=1]) 2012; 116 Wadia (C8NR10447F-(cit22)/*[position()=1]) 2004; 10 Järver (C8NR10447F-(cit4)/*[position()=1]) 2004; 9 Richard (C8NR10447F-(cit21)/*[position()=1]) 2003; 278 Chen (C8NR10447F-(cit32)/*[position()=1]) 2016; 10 Torchilin (C8NR10447F-(cit9)/*[position()=1]) 2003; 100 Sachs (C8NR10447F-(cit41)/*[position()=1]) 2004; 121 Lättig-Tünnemann (C8NR10447F-(cit52)/*[position()=1]) 2011; 2 Vivès (C8NR10447F-(cit7)/*[position()=1]) 2008; 1786 Zorko (C8NR10447F-(cit8)/*[position()=1]) 2005; 57 Di Pisa (C8NR10447F-(cit25)/*[position()=1]) 2014; 54 Säälik (C8NR10447F-(cit2)/*[position()=1]) 2011; 153 Gurtovenko (C8NR10447F-(cit43)/*[position()=1]) 2005; 127 McLaughlin (C8NR10447F-(cit39)/*[position()=1]) 1989; 18 Torchilin (C8NR10447F-(cit6)/*[position()=1]) 2003; 4 Oostenbrink (C8NR10447F-(cit55)/*[position()=1]) 2004; 25 Wallbrecher (C8NR10447F-(cit27)/*[position()=1]) 2017; 256 Tabaei (C8NR10447F-(cit35)/*[position()=1]) 2012; 12 Ciobanasu (C8NR10447F-(cit11)/*[position()=1]) 2010; 99 Berger (C8NR10447F-(cit56)/*[position()=1]) 1997; 72 Darden (C8NR10447F-(cit57)/*[position()=1]) 1993; 98 Lin (C8NR10447F-(cit49)/*[position()=1]) 2013; 7 Herce (C8NR10447F-(cit13)/*[position()=1]) 2009; 97 Kumar (C8NR10447F-(cit62)/*[position()=1]) 1992; 13 Dunkin (C8NR10447F-(cit23)/*[position()=1]) 2010; 115 Herce (C8NR10447F-(cit28)/*[position()=1]) 2007; 104 Tieleman (C8NR10447F-(cit48)/*[position()=1]) 2004; 5 Essmann (C8NR10447F-(cit58)/*[position()=1]) 1995; 103 Delemotte (C8NR10447F-(cit45)/*[position()=1]) 2012; 245 Rao (C8NR10447F-(cit64)/*[position()=1]) 2014; 111 Böckmann (C8NR10447F-(cit50)/*[position()=1]) 2008; 95 Wang (C8NR10447F-(cit65)/*[position()=1]) 2005; 123 Walrant (C8NR10447F-(cit26)/*[position()=1]) 2011; 1808 Kandasamy (C8NR10447F-(cit44)/*[position()=1]) 2006; 125 |
References_xml | – volume: 54 start-page: 194 year: 2014 ident: C8NR10447F-(cit25)/*[position()=1] publication-title: Biochemistry doi: 10.1021/bi501392n contributor: fullname: Di Pisa – volume: 115 start-page: 1188 year: 2010 ident: C8NR10447F-(cit23)/*[position()=1] publication-title: J. Phys. Chem. B doi: 10.1021/jp107763b contributor: fullname: Dunkin – volume: 278 start-page: 34141 year: 2004 ident: C8NR10447F-(cit20)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M303045200 contributor: fullname: Fittipaldi – volume: 44 start-page: 869 year: 2012 ident: C8NR10447F-(cit14)/*[position()=1] publication-title: Int. J. Biochem. Cell Biol. doi: 10.1016/j.biocel.2012.02.010 contributor: fullname: Maniti – volume: 29 start-page: 845 year: 2013 ident: C8NR10447F-(cit54)/*[position()=1] publication-title: Bioinformatics doi: 10.1093/bioinformatics/btt055 contributor: fullname: Pronk – volume: 1808 start-page: 382 year: 2011 ident: C8NR10447F-(cit26)/*[position()=1] publication-title: Biochim. Biophys. Acta, Biomembr. doi: 10.1016/j.bbamem.2010.09.009 contributor: fullname: Walrant – volume: 127 start-page: 17570 year: 2005 ident: C8NR10447F-(cit43)/*[position()=1] publication-title: J. Am. Chem. Soc. doi: 10.1021/ja053129n contributor: fullname: Gurtovenko – volume: 10 start-page: 310 year: 2004 ident: C8NR10447F-(cit22)/*[position()=1] publication-title: Nat. Med. doi: 10.1038/nm996 contributor: fullname: Wadia – volume: 10 start-page: 11541 year: 2016 ident: C8NR10447F-(cit32)/*[position()=1] publication-title: ACS Nano doi: 10.1021/acsnano.6b07563 contributor: fullname: Chen – volume: 278 start-page: 585 year: 2003 ident: C8NR10447F-(cit21)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M209548200 contributor: fullname: Richard – volume: 117 start-page: 230 year: 2013 ident: C8NR10447F-(cit36)/*[position()=1] publication-title: J. Phys. Chem. B doi: 10.1021/jp310255r contributor: fullname: Pourmousa – volume: 4 start-page: 1827 year: 2012 ident: C8NR10447F-(cit10)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl049170q contributor: fullname: Chen – volume: 5 start-page: 10 year: 2004 ident: C8NR10447F-(cit48)/*[position()=1] publication-title: BMC Biochem. doi: 10.1186/1471-2091-5-10 contributor: fullname: Tieleman – volume: 2 start-page: 453 year: 2011 ident: C8NR10447F-(cit52)/*[position()=1] publication-title: Nat. Commun. doi: 10.1038/ncomms1459 contributor: fullname: Lättig-Tünnemann – volume: 89 start-page: 263 year: 2001 ident: C8NR10447F-(cit40)/*[position()=1] publication-title: Adv. Colloid Interface Sci. doi: 10.1016/S0001-8686(00)00061-0 contributor: fullname: Clarke – volume: 104 start-page: 412 year: 2013 ident: C8NR10447F-(cit29)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/j.bpj.2012.10.027 contributor: fullname: Huang – volume: 127 start-page: 17570 year: 2005 ident: C8NR10447F-(cit42)/*[position()=1] publication-title: J. Am. Chem. Soc. doi: 10.1021/ja053129n contributor: fullname: Gurtovenko – volume: 7 start-page: 10799 year: 2013 ident: C8NR10447F-(cit49)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn4040553 contributor: fullname: Lin – volume: 136 start-page: 17459 year: 2014 ident: C8NR10447F-(cit12)/*[position()=1] publication-title: J. Am. Chem. Soc. doi: 10.1021/ja507790z contributor: fullname: Herce – volume: 100 start-page: 11 year: 2014 ident: C8NR10447F-(cit63)/*[position()=1] publication-title: Bioelectrochemistry doi: 10.1016/j.bioelechem.2014.03.009 contributor: fullname: Casciola – volume: 103 start-page: 8577 year: 1995 ident: C8NR10447F-(cit58)/*[position()=1] publication-title: J. Chem. Phys. doi: 10.1063/1.470117 contributor: fullname: Essmann – volume: 108 start-page: 16883 year: 2011 ident: C8NR10447F-(cit15)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1108795108 contributor: fullname: Mishra – volume: 9 start-page: 395 year: 2004 ident: C8NR10447F-(cit4)/*[position()=1] publication-title: Drug Discovery Today doi: 10.1016/S1359-6446(04)03042-9 contributor: fullname: Järver – volume: 256 start-page: 68 year: 2017 ident: C8NR10447F-(cit27)/*[position()=1] publication-title: J. Controlled Release doi: 10.1016/j.jconrel.2017.04.013 contributor: fullname: Wallbrecher – volume: 125 start-page: 074901 year: 2006 ident: C8NR10447F-(cit44)/*[position()=1] publication-title: J. Chem. Phys. doi: 10.1063/1.2217737 contributor: fullname: Kandasamy – volume: 118 start-page: 2670 year: 2014 ident: C8NR10447F-(cit34)/*[position()=1] publication-title: J. Phys. Chem. B doi: 10.1021/jp412600e contributor: fullname: Hu – volume: 98 start-page: 10089 year: 1993 ident: C8NR10447F-(cit57)/*[position()=1] publication-title: J. Chem. Phys. doi: 10.1063/1.464397 contributor: fullname: Darden – volume: 9 start-page: 1281 year: 2012 ident: C8NR10447F-(cit53)/*[position()=1] publication-title: Soft Matter doi: 10.1039/C2SM26519B contributor: fullname: Li – volume: 111 start-page: 12684 year: 2014 ident: C8NR10447F-(cit64)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1411817111 contributor: fullname: Rao – volume: 272 start-page: 16010 year: 1997 ident: C8NR10447F-(cit18)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.272.25.16010 contributor: fullname: Vivès – volume: 153 start-page: 117 year: 2011 ident: C8NR10447F-(cit2)/*[position()=1] publication-title: J. Controlled Release doi: 10.1016/j.jconrel.2011.03.011 contributor: fullname: Säälik – volume: 97 start-page: 1917 year: 2009 ident: C8NR10447F-(cit13)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/j.bpj.2009.05.066 contributor: fullname: Herce – volume: 57 start-page: 529 year: 2005 ident: C8NR10447F-(cit8)/*[position()=1] publication-title: Adv. Drug Delivery Rev. doi: 10.1016/j.addr.2004.10.010 contributor: fullname: Zorko – volume: 1786 start-page: 126 year: 2008 ident: C8NR10447F-(cit7)/*[position()=1] publication-title: Biochim. Biophys. Acta, Rev. Cancer doi: 10.1016/j.bbcan.2008.03.001 contributor: fullname: Vivès – volume: 88 start-page: 4045 year: 2005 ident: C8NR10447F-(cit47)/*[position()=1] publication-title: Biophys. J. doi: 10.1529/biophysj.104.050617 contributor: fullname: Tarek – volume: 95 start-page: 1837 year: 2008 ident: C8NR10447F-(cit50)/*[position()=1] publication-title: Biophys. J. doi: 10.1529/biophysj.108.129437 contributor: fullname: Böckmann – volume: 100 start-page: 1972 year: 2003 ident: C8NR10447F-(cit9)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0435906100 contributor: fullname: Torchilin – volume: 126 start-page: 9506 year: 2004 ident: C8NR10447F-(cit16)/*[position()=1] publication-title: J. Am. Chem. Soc. doi: 10.1021/ja0482536 contributor: fullname: Rothbard – volume: 125 start-page: 6382 year: 2003 ident: C8NR10447F-(cit51)/*[position()=1] publication-title: J. Am. Chem. Soc. doi: 10.1021/ja029504i contributor: fullname: Tieleman – volume: 121 start-page: 10847 year: 2004 ident: C8NR10447F-(cit41)/*[position()=1] publication-title: J. Chem. Phys. doi: 10.1063/1.1826056 contributor: fullname: Sachs – volume: 42 start-page: 13787 year: 2003 ident: C8NR10447F-(cit17)/*[position()=1] publication-title: Biochemistry doi: 10.1021/bi035293y contributor: fullname: Terrone – volume: 10 start-page: 1493 year: 2016 ident: C8NR10447F-(cit33)/*[position()=1] publication-title: ACS Nano doi: 10.1021/acsnano.5b07036 contributor: fullname: Mao – volume: 236 start-page: 27 year: 2010 ident: C8NR10447F-(cit46)/*[position()=1] publication-title: J. Membr. Biol. doi: 10.1007/s00232-010-9277-y contributor: fullname: Levine – volume: 123 start-page: 144104 year: 2005 ident: C8NR10447F-(cit61)/*[position()=1] publication-title: J. Chem. Phys. doi: 10.1063/1.2052648 contributor: fullname: Kästner – volume: 104 start-page: 20805 year: 2007 ident: C8NR10447F-(cit28)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0706574105 contributor: fullname: Herce – volume: 260 start-page: 799 year: 1976 ident: C8NR10447F-(cit38)/*[position()=1] publication-title: Nature doi: 10.1038/260799a0 contributor: fullname: Neher – volume: 4 start-page: 133 year: 2003 ident: C8NR10447F-(cit6)/*[position()=1] publication-title: Curr. Protein Pept. Sci. doi: 10.2174/1389203033487298 contributor: fullname: Torchilin – volume: 25 start-page: 1656–1676 year: 2004 ident: C8NR10447F-(cit55)/*[position()=1] publication-title: J. Comput. Chem. doi: 10.1002/jcc.20090 contributor: fullname: Oostenbrink – volume: 12 start-page: 5719 year: 2012 ident: C8NR10447F-(cit35)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl3029637 contributor: fullname: Tabaei – volume: 18 start-page: 113 year: 1989 ident: C8NR10447F-(cit39)/*[position()=1] publication-title: Annu. Rev. Biophys. Biophys. Chem. doi: 10.1146/annurev.bb.18.060189.000553 contributor: fullname: McLaughlin – volume: 18 start-page: 1463 year: 1997 ident: C8NR10447F-(cit59)/*[position()=1] publication-title: J. Comput. Chem. doi: 10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H contributor: fullname: Hess – volume: 116 start-page: 14353 year: 2012 ident: C8NR10447F-(cit5)/*[position()=1] publication-title: J. Phys. Chem. B doi: 10.1021/jp3095165 contributor: fullname: Rossi – volume: 99 start-page: 153 year: 2010 ident: C8NR10447F-(cit11)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/j.bpj.2010.03.065 contributor: fullname: Ciobanasu – volume: 13 start-page: 1011 year: 1992 ident: C8NR10447F-(cit62)/*[position()=1] publication-title: J. Comput. Chem. doi: 10.1002/jcc.540130812 contributor: fullname: Kumar – volume: 96 start-page: 2658 year: 2009 ident: C8NR10447F-(cit66)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/j.bpj.2008.11.073 contributor: fullname: Grafmüller – volume: 88 start-page: 939 year: 2005 ident: C8NR10447F-(cit31)/*[position()=1] publication-title: Biophys. J. doi: 10.1529/biophysj.104.052787 contributor: fullname: Lensink – volume: 7 start-page: 12906 year: 2016 ident: C8NR10447F-(cit60)/*[position()=1] publication-title: Nat. Commun. doi: 10.1038/ncomms12906 contributor: fullname: Li – volume: 8 start-page: 284 year: 2003 ident: C8NR10447F-(cit19)/*[position()=1] publication-title: Mol. Ther. doi: 10.1016/S1525-0016(03)00122-9 contributor: fullname: Ferrari – volume: 123 start-page: 154701 year: 2005 ident: C8NR10447F-(cit65)/*[position()=1] publication-title: J. Chem. Phys. doi: 10.1063/1.2060666 contributor: fullname: Wang – volume: 97 start-page: 40 year: 2009 ident: C8NR10447F-(cit24)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/j.bpj.2009.03.059 contributor: fullname: Yesylevskyy – volume: 16 start-page: 20785 year: 2014 ident: C8NR10447F-(cit30)/*[position()=1] publication-title: Phys. Chem. Chem. Phys. doi: 10.1039/C4CP02211D contributor: fullname: Sun – volume: 8 start-page: 848 year: 2007 ident: C8NR10447F-(cit1)/*[position()=1] publication-title: Traffic doi: 10.1111/j.1600-0854.2007.00572.x contributor: fullname: Duchardt – volume: 5 start-page: 105 year: 2007 ident: C8NR10447F-(cit3)/*[position()=1] publication-title: Expert Opin. Drug Delivery doi: 10.1517/17425247.5.1.105 contributor: fullname: Foged – volume: 97 start-page: 40 year: 2009 ident: C8NR10447F-(cit37)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/j.bpj.2009.03.059 contributor: fullname: Yesylevskyy – volume: 245 start-page: 531 year: 2012 ident: C8NR10447F-(cit45)/*[position()=1] publication-title: J. Membr. Biol. doi: 10.1007/s00232-012-9434-6 contributor: fullname: Delemotte – volume: 72 start-page: 2002 year: 1997 ident: C8NR10447F-(cit56)/*[position()=1] publication-title: Biophys. J. doi: 10.1016/S0006-3495(97)78845-3 contributor: fullname: Berger |
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SubjectTerms | Drug delivery systems Free energy Ion concentration Molecular dynamics Nucleation Peptides |
Title | Membrane potential drives direct translocation of cell-penetrating peptides |
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