Quantitation of uridine and L-dihydroorotic acid in human plasma by LC–MS/MS using a surrogate matrix approach

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 192; p. 113669
• Main Authors: Yin, Feng, Ling, Yonghua, Martin, Jason, Narayanaswamy, Rohini, McIntosh, Lisa, Li, Fumin, Liu, Guowen
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.01.2021
• Subjects: Biomarker; Dihydroorotate; Human plasma; LC–MS/MS; Target engagement; Uridine; Validation; Validation; Biomarker; Human plasma; Uridine; Target engagement; Dihydroorotate; LC–MS/MS

•A validated LC–MS/MS assay for the quantitation of endogenous analytes, Uridine and L-Dihydroorotic Acid, in human plasma.•A surrogate matrix approach with stable isotope labeled internal standards was used.•Parallelism was successfully demonstrated between the authentic matrix and the surrogate ma...