Fitness of Japanese encephalitis virus to Neuro-2a cells is determined by interactions of the viral envelope protein with highly sulfated glycosaminoglycans on the cell surface

• Published in: Journal of medical virology Vol. 76; no. 4; pp. 583 - 592
• Main Authors: Chiou, Shyan-Song, Liu, Hsuan, Chuang, Ching-Kai, Lin, Chiou-Chun, Chen, Wei-June
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2005; Wiley-Liss
• Subjects: Amino Acid Substitution; Animals; Biological and medical sciences; Cell Line, Tumor; Cell Membrane - chemistry; Cricetinae; DNA Mutational Analysis; Encephalitis Virus, Japanese - genetics; Encephalitis Virus, Japanese - growth & development; envelope; Fundamental and applied biological sciences. Psychology; glycosaminoglycans (GAGs); Glycosaminoglycans - metabolism; Heparitin Sulfate - analysis; Heparitin Sulfate - metabolism; Human viral diseases; Infectious diseases; Japanese encephalitis virus; Medical sciences; Mice; Microbiology; Miscellaneous; Molecular Sequence Data; mutation; Mutation, Missense; neuro-2a cells; Neurons - virology; Sequence Analysis, DNA; Viral diseases; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Viral Plaque Assay; Virology; glycosaminoglycans (GAGs); neuro-2a cells; Flavivirus; Cell surface; Protein; Virus; envelope; Japanese encephalitis virus; Glycosaminoglycan; Japanese encephalitis group virus; Mutation; Flaviviridae; Fitness

Genetically different subpopulations were identified and purified from Japanese Encephalitis virus (JEV). Those with small plaques (SPs; <2 mm in diameter), derived from strains of T1P1, CJN, and CC27, were more competent than those with large plaques (LPs; >5 mm in diameter) when passaged in...