Software-assisted morphometric and phenotype analyses of human peripheral blood monocyte-derived macrophages induced by a microenvironment model of human esophageal squamous cell carcinoma

• Published in: Pathology international Vol. 66; no. 2; pp. 83 - 93
• Main Authors: Nishio, Mari, Urakawa, Naoki, Shigeoka, Manabu, Takase, Nobuhisa, Ichihara, Yumi, Arai, Noriaki, Koma, Yu-ichiro, Yokozaki, Hiroshi
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.02.2016
• Subjects: Antigens, CD - metabolism; Antigens, Differentiation, Myelomonocytic - metabolism; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Differentiation; Cell Line, Tumor; Culture Media, Conditioned; esophageal cancer; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma; Humans; image analysis; Image Processing, Computer-Assisted; Interleukin-4 - pharmacology; macrophages; Macrophages - metabolism; Macrophages - pathology; Monocytes - metabolism; Monocytes - pathology; Phenotype; Receptors, Cell Surface - metabolism; Scavenger Receptors, Class A - metabolism; Software; Tumor Microenvironment; macrophages; esophageal cancer; tumor microenvironment; image analysis

Human macrophages play important roles in tumor promotion and are called tumor‐associated macrophages (TAMs). We previously demonstrated that human esophageal squamous cell carcinomas (ESCCs) contain TAMs and that these TAMs tend to have tumor‐supporting features. Here we exposed human macrophages t...