Long-Read Sequencing – A Powerful Tool in Viral Transcriptome Research

• Published in: Trends in microbiology (Regular ed.) Vol. 27; no. 7; pp. 578 - 592
• Main Authors: Boldogkői, Zsolt, Moldován, Norbert, Balázs, Zsolt, Snyder, Michael, Tombácz, Dóra
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2019; Elsevier Science Ltd
• Subjects: Deoxyribonucleic acid; DNA; DNA replication; Gene expression; genome; Genomes; Identification methods; Isoforms; messenger RNA; nanopore sequencing; nanopores; noncoding RNA; Pacific Biosciences; Porosity; Production methods; replication origin; Ribonucleic acid; RNA; RNA viruses; RNA-Seq; splicing; Transcription; transcription (genetics); transcriptome; transcriptomics; Viruses; nanopore sequencing; DNA replication; noncoding RNA; RNA-Seq; splicing; replication origin; Pacific Biosciences

Long-read sequencing (LRS) has become increasingly popular due to its strengths in de novo assembly and in resolving complex DNA regions as well as in determining full-length RNA molecules. Two important LRS technologies have been developed during the past few years, including single-molecule, real-...