Differential epigenetic regulation of BDNF and NT-3 genes by trichostatin A and 5-aza-2′-deoxycytidine in Neuro-2a cells

To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite gen...

Full description

Saved in:

Bibliographic Details
Published in	Biochemical and biophysical research communications Vol. 394; no. 1; pp. 173 - 177
Main Authors	Ishimaru, Naoki, Fukuchi, Mamoru, Hirai, Akina, Chiba, Yusuke, Tamura, Tomonari, Takahashi, Nami, Tabuchi, Akiko, Tsuda, Masaaki, Shiraishi, Masahiko
Format	Journal Article
Language	English
Published	United States Elsevier Inc 26.03.2010
Subjects	Acetylation Animals Azacitidine - analogs & derivatives Azacitidine - pharmacology BDNF Brain-Derived Neurotrophic Factor - genetics Cell Line, Tumor Chromatin Immunoprecipitation CpG Islands DNA Methylation DNA Modification Methylases - antagonists & inhibitors Epigenesis, Genetic Exons - genetics Histone acetylation Histones - metabolism Hydroxamic Acids - pharmacology Mice Nerve Growth Factors - genetics Neurons - drug effects Neurons - metabolism NT-3 Promoter Regions, Genetic DNA methylation NT-3 Histone acetylation BDNF
Online Access	Get full text

Cover

Loading…

Abstract	To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2′-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exon I–IX mRNA was induced by trichostatin A (TSA) treatment. However, NT-3 exon IB–II mRNA was not induced by TSA treatment. Chromatin immunoprecipitation assays showed that the levels of acetylated histones H3 and H4 on BDNF promoter I were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells.
AbstractList	To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2′-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exon I–IX mRNA was induced by trichostatin A (TSA) treatment. However, NT-3 exon IB–II mRNA was not induced by TSA treatment. Chromatin immunoprecipitation assays showed that the levels of acetylated histones H3 and H4 on BDNF promoter I were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells. To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2'-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exon I-IX mRNA was induced by trichostatin A (TSA) treatment. However, NT-3 exon IB-II mRNA was not induced by TSA treatment. Chromatin immunoprecipitation assays showed that the levels of acetylated histones H3 and H4 on BDNF promoter I were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells.To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2'-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exon I-IX mRNA was induced by trichostatin A (TSA) treatment. However, NT-3 exon IB-II mRNA was not induced by TSA treatment. Chromatin immunoprecipitation assays showed that the levels of acetylated histones H3 and H4 on BDNF promoter I were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells.
Author	Hirai, Akina Chiba, Yusuke Takahashi, Nami Shiraishi, Masahiko Ishimaru, Naoki Tamura, Tomonari Fukuchi, Mamoru Tsuda, Masaaki Tabuchi, Akiko
Author_xml	– sequence: 1 givenname: Naoki surname: Ishimaru fullname: Ishimaru, Naoki organization: Faculty of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Ootawara, Tochigi 324-8501, Japan – sequence: 2 givenname: Mamoru surname: Fukuchi fullname: Fukuchi, Mamoru organization: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan – sequence: 3 givenname: Akina surname: Hirai fullname: Hirai, Akina organization: Faculty of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Ootawara, Tochigi 324-8501, Japan – sequence: 4 givenname: Yusuke surname: Chiba fullname: Chiba, Yusuke organization: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan – sequence: 5 givenname: Tomonari surname: Tamura fullname: Tamura, Tomonari organization: Faculty of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Ootawara, Tochigi 324-8501, Japan – sequence: 6 givenname: Nami surname: Takahashi fullname: Takahashi, Nami organization: Faculty of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Ootawara, Tochigi 324-8501, Japan – sequence: 7 givenname: Akiko surname: Tabuchi fullname: Tabuchi, Akiko organization: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan – sequence: 8 givenname: Masaaki surname: Tsuda fullname: Tsuda, Masaaki organization: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan – sequence: 9 givenname: Masahiko surname: Shiraishi fullname: Shiraishi, Masahiko email: mshirais@iuhw.ac.jp organization: Faculty of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Ootawara, Tochigi 324-8501, Japan
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/20188708$$D View this record in MEDLINE/PubMed
BookMark	eNp9kc1uEzEURi1URNPCC7BA3rFyuLYnkxmJTWkpIFVhUyR2ln_uFEcTO9hORbrimXgkngRP025YdHXlq3OurO87IUchBiTkNYc5B96-W8-NSXYuoC5AzLnsn5EZhx6Y4NAckRkAtEz0_PsxOcl5DcB50_YvyHFVum4J3YzcXfhhwISheD1S3PobDFi8pQlvdqMuPgYaB_rhYnVJdXB0dc0knZhMzZ6W5O2PmEvlAj27BxZM32km_v7-wxzGX3u7L975gLQSK9ylyISmFscxvyTPBz1mfPUwT8m3y4_X55_Z1ddPX87PrphtBC_MNXYw2JjGtcJ2zbCULQhsnJGdAS5RmM709Qmi5Z3Rsh9M3y7ALRqJy6Fip-Tt4e42xZ87zEVtfJ5-oAPGXVZLKTvZg1xU8s0DuTMbdGqb_EanvXqMqwLdAbAp5pxwUNaX-5BK0n5UHNTUjFqrqZnJAwVC1WaqKv5TH68_Kb0_SFjzufWYVLYeg0XnE9qiXPRP6f8ASmKnrg
CitedBy_id	crossref_primary_10_1007_s12177_012_9080_3 crossref_primary_10_1089_thy_2017_0331 crossref_primary_10_1111_jcmm_15358 crossref_primary_10_1016_j_neuropharm_2016_01_025 crossref_primary_10_1080_1028415X_2017_1312090 crossref_primary_10_1097_WNR_0b013e3283595709 crossref_primary_10_1523_JNEUROSCI_0260_15_2015 crossref_primary_10_1007_s12035_016_0357_6 crossref_primary_10_4103_mgmj_mgmj_90_20 crossref_primary_10_1517_17460441_2014_930124 crossref_primary_10_1016_j_neuint_2024_105889 crossref_primary_10_1016_j_neures_2013_08_004 crossref_primary_10_1038_mp_2011_107 crossref_primary_10_1016_j_neuroscience_2013_02_053 crossref_primary_10_1038_jhg_2013_65 crossref_primary_10_1186_s13041_018_0380_9 crossref_primary_10_3390_ijms161226236 crossref_primary_10_1016_j_pneurobio_2015_05_002 crossref_primary_10_1371_journal_pone_0022550 crossref_primary_10_3390_ijerph17134878 crossref_primary_10_1007_s11357_011_9375_5 crossref_primary_10_1002_ddr_21219 crossref_primary_10_1016_j_bbrc_2020_06_064 crossref_primary_10_1111_jnc_15122 crossref_primary_10_1038_srep20957 crossref_primary_10_1172_JCI154612 crossref_primary_10_1186_2040_2392_4_46 crossref_primary_10_3389_fgene_2018_00579 crossref_primary_10_2174_1389450124666221207090108 crossref_primary_10_2174_1871527320666210825101915 crossref_primary_10_1038_s41398_020_01037_4 crossref_primary_10_3389_fnagi_2020_00211 crossref_primary_10_3390_ijms18030571 crossref_primary_10_1007_s40495_021_00265_6 crossref_primary_10_1016_j_arr_2013_05_003
Cites_doi	10.1523/JNEUROSCI.4119-05.2005 10.1517/14728222.12.4.437 10.1126/science.270.5236.593 10.1111/j.1460-9568.1994.tb00613.x 10.1016/j.molbrainres.2005.06.014 10.1016/j.neures.2009.05.002 10.1002/j.1460-2075.1982.tb01207.x 10.1111/j.1471-4159.2008.05237.x 10.1111/j.1471-4159.2006.04216.x 10.1042/CS20050163 10.1073/pnas.191352298 10.1016/S0896-6273(01)00561-X 10.1038/nrn1806 10.1002/jnr.21139 10.1093/dnares/11.6.409 10.1046/j.1471-4159.2000.0750041.x 10.1038/nrn2132 10.1080/08977190410001723308 10.1523/JNEUROSCI.1786-08.2008 10.1126/science.7907431 10.1523/JNEUROSCI.23-07-02572.2003 10.1196/annals.1403.009 10.1126/science.1090842 10.1126/science.1086446 10.1016/0896-6273(93)90335-O 10.1074/jbc.M204784200 10.1016/S0896-6273(00)81011-9 10.1074/jbc.M302879200
ContentType	Journal Article
Copyright	2010 Elsevier Inc. Copyright (c) 2010 Elsevier Inc. All rights reserved.
Copyright_xml	– notice: 2010 Elsevier Inc. – notice: Copyright (c) 2010 Elsevier Inc. All rights reserved.
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1016/j.bbrc.2010.02.139
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE MEDLINE - Academic
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Anatomy & Physiology Chemistry Biology
EISSN	1090-2104
EndPage	177
ExternalDocumentID	20188708 10_1016_j_bbrc_2010_02_139 S0006291X10003736
Genre	Journal Article
GroupedDBID	--- --K --M -~X .~1 0R~ 1B1 1RT 1~. 1~5 23N 4.4 457 4G. 53G 5GY 5VS 6J9 7-5 71M 8P~ 9JM 9M8 AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAXUO ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACNCT ACRLP ADBBV ADEZE ADIYS ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AGHFR AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC CS3 D0L DM4 DOVZS EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FYGXN G-Q HZ~ IHE J1W K-O KOM L7B LG5 LX2 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 RIG RNS ROL RPZ SCC SDF SDG SDP SES SPCBC SSU SSZ T5K TWZ WH7 XPP XSW ZA5 ZMT ~02 ~G- .55 .GJ .HR 1CY 3O- AAHBH AAQXK AATTM AAXKI AAYJJ AAYWO AAYXX ABDPE ABEFU ABWVN ACKIV ACRPL ACVFH ADCNI ADFGL ADNMO AEIPS AEUPX AFJKZ AFPUW AGCQF AGQPQ AGRDE AGRNS AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP ASPBG AVWKF AZFZN BNPGV CAG CITATION COF FEDTE FGOYB G-2 GBLVA HLW HVGLF MVM OHT R2- SBG SEW SSH UQL WUQ X7M Y6R ZGI ZKB ~KM CGR CUY CVF ECM EFKBS EIF NPM 7X8
ID	FETCH-LOGICAL-c421t-d4cfbe4b4d62c84f73602e4db38b013e2b8b94db02618ba39fb9650d543e7fdb3
IEDL.DBID	AIKHN
ISSN	0006-291X 1090-2104
IngestDate	Fri Jul 11 06:15:55 EDT 2025 Mon Jul 21 05:59:11 EDT 2025 Thu Apr 24 22:56:38 EDT 2025 Tue Jul 01 04:24:04 EDT 2025 Fri Feb 23 02:34:27 EST 2024
IsPeerReviewed	true
IsScholarly	true
Issue	1
Keywords	DNA methylation NT-3 Histone acetylation BDNF
Language	English
License	https://www.elsevier.com/tdm/userlicense/1.0 Copyright (c) 2010 Elsevier Inc. All rights reserved.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c421t-d4cfbe4b4d62c84f73602e4db38b013e2b8b94db02618ba39fb9650d543e7fdb3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
PMID	20188708
PQID	733839035
PQPubID	23479
PageCount	5
ParticipantIDs	proquest_miscellaneous_733839035 pubmed_primary_20188708 crossref_citationtrail_10_1016_j_bbrc_2010_02_139 crossref_primary_10_1016_j_bbrc_2010_02_139 elsevier_sciencedirect_doi_10_1016_j_bbrc_2010_02_139
ProviderPackageCode	CITATION AAYXX
PublicationCentury	2000
PublicationDate	2010-03-26
PublicationDateYYYYMMDD	2010-03-26
PublicationDate_xml	– month: 03 year: 2010 text: 2010-03-26 day: 26
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	Biochemical and biophysical research communications
PublicationTitleAlternate	Biochem Biophys Res Commun
PublicationYear	2010
Publisher	Elsevier Inc
Publisher_xml	– name: Elsevier Inc
References	Martinowich, Hattori, Wu, Fouse, He, Hu, Fan, Sun (bib24) 2003; 302 Aid, Kazantseva, Piirsoo, Palm, Timmusk (bib10) 2007; 85 Fukuchi, Nii, Ishimaru, Minamino, Hara, Takasaki, Tabuchi, Tsuda (bib26) 2009; 65 Chen, Chang, Lin, Meissner, West, Griffith, Jaenisch, Greenberg (bib25) 2003; 302 Thoenen (bib2) 1995; 270 Dennis, Levitt (bib20) 2005; 140 Tao, West, Chen, Corfas, Greenberg (bib19) 2002; 33 Colvis, Pollock, Goodman, Impey, Dunn, Mandel, Champagne, Mayford, Korzus, Kumar, Renthal, Theobald, Nestler (bib27) 2005; 25 Shiraishi, Hayatsu (bib23) 2004; 11 Lubin, Roth, Sweatt (bib21) 2008; 28 Binder, Scharfman (bib4) 2004; 22 Lipsky, Marini (bib3) 2007; 1122 Evans, Barker (bib6) 2008; 12 Tabuchi, Sakaya, Kisukeda, Fushiki, Tsuda (bib17) 2002; 277 West, Chen, Dalva, Dolmetsch, Kornhauser, Shaywitz, Takasu, Tao, Greenberg (bib12) 2001; 98 Kendall, Yeo, Henttu, Tomlinson (bib14) 2000; 75 Leingärtner, Lindholm (bib13) 1994; 6 Ishimaru, Tabuchi, Hara, Hayashi, Sugimoto, Yasuhara, Shiota, Tsuda (bib15) 2007; 100 Chen, West, Tao, Corfas, Szentirmay, Sawadogo, Vinson, Greenberg (bib18) 2003; 23 Hu, Russek (bib8) 2008; 105 Shieh, Hu, Bobb, Timmusk, Ghosh (bib16) 1998; 20 Cattaneo, Zuccato, Tartari (bib7) 2005; 6 Wang, Wei, Loh (bib22) 2003; 278 Barde, Edgar, Thoenen (bib1) 1982; 1 Tsankova, Renthal, Kumar, Nestler (bib28) 2007; 8 Chao, Rajagopal, Lee (bib5) 2006; 110 Ghosh, Carnahan, Greenberg (bib11) 1994; 263 Timmusk, Palm, Metsis, Reintam, Paalme, Saarma, Persson (bib9) 1993; 10 Wang (10.1016/j.bbrc.2010.02.139_bib22) 2003; 278 Ishimaru (10.1016/j.bbrc.2010.02.139_bib15) 2007; 100 Shieh (10.1016/j.bbrc.2010.02.139_bib16) 1998; 20 Binder (10.1016/j.bbrc.2010.02.139_bib4) 2004; 22 Tao (10.1016/j.bbrc.2010.02.139_bib19) 2002; 33 Chen (10.1016/j.bbrc.2010.02.139_bib25) 2003; 302 Chao (10.1016/j.bbrc.2010.02.139_bib5) 2006; 110 Hu (10.1016/j.bbrc.2010.02.139_bib8) 2008; 105 Shiraishi (10.1016/j.bbrc.2010.02.139_bib23) 2004; 11 Colvis (10.1016/j.bbrc.2010.02.139_bib27) 2005; 25 Tsankova (10.1016/j.bbrc.2010.02.139_bib28) 2007; 8 Lipsky (10.1016/j.bbrc.2010.02.139_bib3) 2007; 1122 Barde (10.1016/j.bbrc.2010.02.139_bib1) 1982; 1 Dennis (10.1016/j.bbrc.2010.02.139_bib20) 2005; 140 Tabuchi (10.1016/j.bbrc.2010.02.139_bib17) 2002; 277 Timmusk (10.1016/j.bbrc.2010.02.139_bib9) 1993; 10 Thoenen (10.1016/j.bbrc.2010.02.139_bib2) 1995; 270 Cattaneo (10.1016/j.bbrc.2010.02.139_bib7) 2005; 6 Lubin (10.1016/j.bbrc.2010.02.139_bib21) 2008; 28 Chen (10.1016/j.bbrc.2010.02.139_bib18) 2003; 23 Martinowich (10.1016/j.bbrc.2010.02.139_bib24) 2003; 302 Evans (10.1016/j.bbrc.2010.02.139_bib6) 2008; 12 Kendall (10.1016/j.bbrc.2010.02.139_bib14) 2000; 75 Fukuchi (10.1016/j.bbrc.2010.02.139_bib26) 2009; 65 Leingärtner (10.1016/j.bbrc.2010.02.139_bib13) 1994; 6 West (10.1016/j.bbrc.2010.02.139_bib12) 2001; 98 Ghosh (10.1016/j.bbrc.2010.02.139_bib11) 1994; 263 Aid (10.1016/j.bbrc.2010.02.139_bib10) 2007; 85
References_xml	– volume: 28 start-page: 10576 year: 2008 end-page: 10586 ident: bib21 article-title: Epigenetic regulation of BDNF gene transcription in the consolidation of fear memory publication-title: J. Neurosci. – volume: 1 start-page: 549 year: 1982 end-page: 553 ident: bib1 article-title: Purification of a new neurotrophic factor from mammalian brain publication-title: EMBO J. – volume: 277 start-page: 35920 year: 2002 end-page: 35931 ident: bib17 article-title: Involvement of an upstream stimulatory factor as well as cAMP-responsive element-binding protein in the activation of brain-derived neurotrophic factor gene promoter I publication-title: J. Biol. Chem. – volume: 65 start-page: 35 year: 2009 end-page: 43 ident: bib26 article-title: Valproic acid induces up- or down-regulation of gene expression responsible for the neuronal excitation and inhibition in rat cortical neurons through its epigenetic actions publication-title: Neurosci. Res. – volume: 270 start-page: 593 year: 1995 end-page: 598 ident: bib2 article-title: Neurotrophins and neuronal plasticity publication-title: Science – volume: 8 start-page: 355 year: 2007 end-page: 367 ident: bib28 article-title: Epigenetic regulation in psychiatric disorders publication-title: Nat. Rev. Neurosci. – volume: 75 start-page: 41 year: 2000 end-page: 47 ident: bib14 article-title: Alternative splicing of the neurotrophin-3 gene gives rise to different transcripts in a number of human and rat tissues publication-title: J. Neurochem. – volume: 1122 start-page: 130 year: 2007 end-page: 143 ident: bib3 article-title: Brain-derived neurotrophic factor in neuronal survival and behavior-related plasticity publication-title: Ann. NY Acad. Sci. – volume: 302 start-page: 890 year: 2003 end-page: 893 ident: bib24 article-title: DNA methylation-related chromatin remodeling in activity-dependent BDNF gene regulation publication-title: Science – volume: 105 start-page: 1 year: 2008 end-page: 17 ident: bib8 article-title: BDNF and the diseased nervous system: a delicate balance between adaptive and pathological processes of gene regulation publication-title: J. Neurochem. – volume: 6 start-page: 919 year: 2005 end-page: 930 ident: bib7 article-title: Normal huntingtin function: an alternative approach to Huntington’s disease publication-title: Nat. Rev. Neurosci. – volume: 140 start-page: 1 year: 2005 end-page: 9 ident: bib20 article-title: Regional expression of brain derived neurotrophic factor (BDNF) is correlated with dynamic patterns of promoter methylation in the developing mouse forebrain publication-title: Brain Res. Mol. Brain Res. – volume: 85 start-page: 525 year: 2007 end-page: 535 ident: bib10 article-title: Mouse and rat BDNF gene structure and expression revisited publication-title: J. Neurosci. Res. – volume: 11 start-page: 409 year: 2004 end-page: 415 ident: bib23 article-title: High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation publication-title: DNA Res. – volume: 302 start-page: 885 year: 2003 end-page: 889 ident: bib25 article-title: Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2 publication-title: Science – volume: 6 start-page: 1149 year: 1994 end-page: 1159 ident: bib13 article-title: Two promoters direct transcription of the mouse NT-3 gene publication-title: Eur. J. Neurosci. – volume: 33 start-page: 383 year: 2002 end-page: 395 ident: bib19 article-title: A calcium-responsive transcription factor, CaRF, that regulates neuronal activity-dependent expression of BDNF publication-title: Neuron – volume: 25 start-page: 10379 year: 2005 end-page: 10389 ident: bib27 article-title: Epigenetic mechanisms and gene networks in the nervous system publication-title: J. Neurosci. – volume: 100 start-page: 520 year: 2007 end-page: 531 ident: bib15 article-title: Regulation of neurotrophin-3 gene transcription by Sp3 and Sp4 in neurons publication-title: J. Neurochem. – volume: 20 start-page: 727 year: 1998 end-page: 740 ident: bib16 article-title: Identification of a signaling pathway involved in calcium regulation of BDNF expression publication-title: Neuron – volume: 10 start-page: 475 year: 1993 end-page: 489 ident: bib9 article-title: Multiple promoters direct tissue-specific expression of the rat BDNF gene publication-title: Neuron – volume: 22 start-page: 123 year: 2004 end-page: 131 ident: bib4 article-title: Brain-derived neurotrophic factor publication-title: Growth Factors – volume: 23 start-page: 2572 year: 2003 end-page: 2581 ident: bib18 article-title: Upstream stimulatory factors are mediators of Ca publication-title: J. Neurosci. – volume: 12 start-page: 437 year: 2008 end-page: 447 ident: bib6 article-title: Neurotrophic factors as a therapeutic target for Parkinson’s disease publication-title: Expert Opin. Ther. Targets – volume: 110 start-page: 167 year: 2006 end-page: 173 ident: bib5 article-title: Neurotrophin signalling in health and disease publication-title: Clin. Sci. (Lond.) – volume: 98 start-page: 11024 year: 2001 end-page: 11031 ident: bib12 article-title: Calcium regulation of neuronal gene expression publication-title: Proc. Natl. Acad. Sci. USA – volume: 263 start-page: 1618 year: 1994 end-page: 1623 ident: bib11 article-title: Requirement for BDNF in activity-dependent survival of cortical neurons publication-title: Science – volume: 278 start-page: 40550 year: 2003 end-page: 40556 ident: bib22 article-title: Transcriptional regulation of mouse delta-opioid receptor gene by CpG methylation: involvement of Sp3 and a methyl-CpG-binding protein, MBD2, in transcriptional repression of mouse delta-opioid receptor gene in Neuro2A cells publication-title: J. Biol. Chem. – volume: 25 start-page: 10379 year: 2005 ident: 10.1016/j.bbrc.2010.02.139_bib27 article-title: Epigenetic mechanisms and gene networks in the nervous system publication-title: J. Neurosci. doi: 10.1523/JNEUROSCI.4119-05.2005 – volume: 12 start-page: 437 year: 2008 ident: 10.1016/j.bbrc.2010.02.139_bib6 article-title: Neurotrophic factors as a therapeutic target for Parkinson’s disease publication-title: Expert Opin. Ther. Targets doi: 10.1517/14728222.12.4.437 – volume: 270 start-page: 593 year: 1995 ident: 10.1016/j.bbrc.2010.02.139_bib2 article-title: Neurotrophins and neuronal plasticity publication-title: Science doi: 10.1126/science.270.5236.593 – volume: 6 start-page: 1149 year: 1994 ident: 10.1016/j.bbrc.2010.02.139_bib13 article-title: Two promoters direct transcription of the mouse NT-3 gene publication-title: Eur. J. Neurosci. doi: 10.1111/j.1460-9568.1994.tb00613.x – volume: 140 start-page: 1 year: 2005 ident: 10.1016/j.bbrc.2010.02.139_bib20 article-title: Regional expression of brain derived neurotrophic factor (BDNF) is correlated with dynamic patterns of promoter methylation in the developing mouse forebrain publication-title: Brain Res. Mol. Brain Res. doi: 10.1016/j.molbrainres.2005.06.014 – volume: 65 start-page: 35 year: 2009 ident: 10.1016/j.bbrc.2010.02.139_bib26 article-title: Valproic acid induces up- or down-regulation of gene expression responsible for the neuronal excitation and inhibition in rat cortical neurons through its epigenetic actions publication-title: Neurosci. Res. doi: 10.1016/j.neures.2009.05.002 – volume: 1 start-page: 549 year: 1982 ident: 10.1016/j.bbrc.2010.02.139_bib1 article-title: Purification of a new neurotrophic factor from mammalian brain publication-title: EMBO J. doi: 10.1002/j.1460-2075.1982.tb01207.x – volume: 105 start-page: 1 year: 2008 ident: 10.1016/j.bbrc.2010.02.139_bib8 article-title: BDNF and the diseased nervous system: a delicate balance between adaptive and pathological processes of gene regulation publication-title: J. Neurochem. doi: 10.1111/j.1471-4159.2008.05237.x – volume: 100 start-page: 520 year: 2007 ident: 10.1016/j.bbrc.2010.02.139_bib15 article-title: Regulation of neurotrophin-3 gene transcription by Sp3 and Sp4 in neurons publication-title: J. Neurochem. doi: 10.1111/j.1471-4159.2006.04216.x – volume: 110 start-page: 167 year: 2006 ident: 10.1016/j.bbrc.2010.02.139_bib5 article-title: Neurotrophin signalling in health and disease publication-title: Clin. Sci. (Lond.) doi: 10.1042/CS20050163 – volume: 98 start-page: 11024 year: 2001 ident: 10.1016/j.bbrc.2010.02.139_bib12 article-title: Calcium regulation of neuronal gene expression publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.191352298 – volume: 33 start-page: 383 year: 2002 ident: 10.1016/j.bbrc.2010.02.139_bib19 article-title: A calcium-responsive transcription factor, CaRF, that regulates neuronal activity-dependent expression of BDNF publication-title: Neuron doi: 10.1016/S0896-6273(01)00561-X – volume: 6 start-page: 919 year: 2005 ident: 10.1016/j.bbrc.2010.02.139_bib7 article-title: Normal huntingtin function: an alternative approach to Huntington’s disease publication-title: Nat. Rev. Neurosci. doi: 10.1038/nrn1806 – volume: 85 start-page: 525 year: 2007 ident: 10.1016/j.bbrc.2010.02.139_bib10 article-title: Mouse and rat BDNF gene structure and expression revisited publication-title: J. Neurosci. Res. doi: 10.1002/jnr.21139 – volume: 11 start-page: 409 year: 2004 ident: 10.1016/j.bbrc.2010.02.139_bib23 article-title: High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation publication-title: DNA Res. doi: 10.1093/dnares/11.6.409 – volume: 75 start-page: 41 year: 2000 ident: 10.1016/j.bbrc.2010.02.139_bib14 article-title: Alternative splicing of the neurotrophin-3 gene gives rise to different transcripts in a number of human and rat tissues publication-title: J. Neurochem. doi: 10.1046/j.1471-4159.2000.0750041.x – volume: 8 start-page: 355 year: 2007 ident: 10.1016/j.bbrc.2010.02.139_bib28 article-title: Epigenetic regulation in psychiatric disorders publication-title: Nat. Rev. Neurosci. doi: 10.1038/nrn2132 – volume: 22 start-page: 123 year: 2004 ident: 10.1016/j.bbrc.2010.02.139_bib4 article-title: Brain-derived neurotrophic factor publication-title: Growth Factors doi: 10.1080/08977190410001723308 – volume: 28 start-page: 10576 year: 2008 ident: 10.1016/j.bbrc.2010.02.139_bib21 article-title: Epigenetic regulation of BDNF gene transcription in the consolidation of fear memory publication-title: J. Neurosci. doi: 10.1523/JNEUROSCI.1786-08.2008 – volume: 263 start-page: 1618 year: 1994 ident: 10.1016/j.bbrc.2010.02.139_bib11 article-title: Requirement for BDNF in activity-dependent survival of cortical neurons publication-title: Science doi: 10.1126/science.7907431 – volume: 23 start-page: 2572 year: 2003 ident: 10.1016/j.bbrc.2010.02.139_bib18 article-title: Upstream stimulatory factors are mediators of Ca2+-responsive transcription in neurons publication-title: J. Neurosci. doi: 10.1523/JNEUROSCI.23-07-02572.2003 – volume: 1122 start-page: 130 year: 2007 ident: 10.1016/j.bbrc.2010.02.139_bib3 article-title: Brain-derived neurotrophic factor in neuronal survival and behavior-related plasticity publication-title: Ann. NY Acad. Sci. doi: 10.1196/annals.1403.009 – volume: 302 start-page: 890 year: 2003 ident: 10.1016/j.bbrc.2010.02.139_bib24 article-title: DNA methylation-related chromatin remodeling in activity-dependent BDNF gene regulation publication-title: Science doi: 10.1126/science.1090842 – volume: 302 start-page: 885 year: 2003 ident: 10.1016/j.bbrc.2010.02.139_bib25 article-title: Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2 publication-title: Science doi: 10.1126/science.1086446 – volume: 10 start-page: 475 year: 1993 ident: 10.1016/j.bbrc.2010.02.139_bib9 article-title: Multiple promoters direct tissue-specific expression of the rat BDNF gene publication-title: Neuron doi: 10.1016/0896-6273(93)90335-O – volume: 277 start-page: 35920 year: 2002 ident: 10.1016/j.bbrc.2010.02.139_bib17 article-title: Involvement of an upstream stimulatory factor as well as cAMP-responsive element-binding protein in the activation of brain-derived neurotrophic factor gene promoter I publication-title: J. Biol. Chem. doi: 10.1074/jbc.M204784200 – volume: 20 start-page: 727 year: 1998 ident: 10.1016/j.bbrc.2010.02.139_bib16 article-title: Identification of a signaling pathway involved in calcium regulation of BDNF expression publication-title: Neuron doi: 10.1016/S0896-6273(00)81011-9 – volume: 278 start-page: 40550 year: 2003 ident: 10.1016/j.bbrc.2010.02.139_bib22 article-title: Transcriptional regulation of mouse delta-opioid receptor gene by CpG methylation: involvement of Sp3 and a methyl-CpG-binding protein, MBD2, in transcriptional repression of mouse delta-opioid receptor gene in Neuro2A cells publication-title: J. Biol. Chem. doi: 10.1074/jbc.M302879200
SSID	ssj0011469
Score	2.1525052
Snippet	To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived...
SourceID	proquest pubmed crossref elsevier
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	173
SubjectTerms	Acetylation Animals Azacitidine - analogs & derivatives Azacitidine - pharmacology BDNF Brain-Derived Neurotrophic Factor - genetics Cell Line, Tumor Chromatin Immunoprecipitation CpG Islands DNA Methylation DNA Modification Methylases - antagonists & inhibitors Epigenesis, Genetic Exons - genetics Histone acetylation Histones - metabolism Hydroxamic Acids - pharmacology Mice Nerve Growth Factors - genetics Neurons - drug effects Neurons - metabolism NT-3 Promoter Regions, Genetic
Title	Differential epigenetic regulation of BDNF and NT-3 genes by trichostatin A and 5-aza-2′-deoxycytidine in Neuro-2a cells
URI	https://dx.doi.org/10.1016/j.bbrc.2010.02.139 https://www.ncbi.nlm.nih.gov/pubmed/20188708 https://www.proquest.com/docview/733839035
Volume	394
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9tAEF7yoLSX0iZ9uI-wh9JL2UT70Fp7dJwatwWfEvBt0eyuwCWRje1AnUPpb8pPyi_prLQyFJIcepPEDBLzjWZmd-dByCeouKi4L5guhWLKFMAQZs58X-YOtIoFjzHbYqLHF-r7NJ_ukGFXCxPTKpPtb216Y63Tk5MkzZPFbBZrfDMtDJ_ypomK1LtkX0ijUbX3B99-jCfbwwQ0BikK1iwypNqZNs0LYOlShpc45nFm-P3-6aH4s_FDoxfkeQog6aD9xpdkJ9QH5HBQ4-L5akM_0yals9krPyBPTrurp8NusNshuTlLQ1Hw576kYRH7ccZSRrps59IjUnRe0dOzyYiWtaeTcyZppFlR2NDY0T9WhiBdTQcNQc7Km5KJuz-3zIf5r43brGfoEgNFiqb5BxMljScEq1fkYvT1fDhmaQQDc0rwNfPKVRAUKK-FK1SFYs5EUB5k0WygCijA4G1cyRVQSlOBwZjP50qGfoVkr8lePa_DW0KNBpGDqQIYpyAXhXdV7kvuBA_SQ9kjvBO8dak_eRyTcWm7RLSfNoJlI1g2ExbB6pEvW55F253jUeq8w9P-o2MW3cejfLQD3yJUUV5lHebXK9uPC3yTybxH3rRKsf0M5Ef7nRXv_vOl78mzNlFBMqE_kL318jp8xPhnDUdk9_g3P0pa_hfFyAPu
linkProvider	Elsevier
linkToHtml	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LbxMxELZKESoXBC2P8PQBcUFu117vZn1MU6IAJadUys3a8UNKVTZRkkqkB8Rv4ifxS_B4vZGQoAduu6uxdjWfPTP2fjNDyFvwXHhuK1bWQjKpKmABZs5sPy8MlBITHpFtMSnHF_LTrJjtkWGXC4O0ymT7W5serXV6cpK0ebKczzHHNyuF4jMei6jk5R1yV4bli6vz-PuO54FZtykGLhmKp8yZluQFsDKJ3yWOOXYM_7t3-lf0Gb3Q6CF5kMJHOmi_8BHZc80hORo0Yev8dUvf0UjojCflh-TeaXd1MOzauh2Rm7PUEiUs7SvqlliNExMZ6artSh9wogtPT88mI1o3lk6mLKcos6awpVjPH_NCglxDB1GgYPVNzcSvHz-ZdYtvW7PdzINDdDRIxNIfTNQU_w-sH5OL0YfpcMxSAwZmpOAbZqXx4CRIWwpTSR-UnAknLeRVPD4VUIEKt7iPq6DOlQcVIj5byNz1fRB7QvabReOeEapKEAUo70AZCYWorPGFrbkR3OUW6h7hneK1SdXJsUnGle5oaJcawdIIls6EDmD1yPvdmGVbm-NW6aLDU_8xw3RwHreOox34OkCF-qobt7he6z5u71WWFz3ytJ0Uu88I44P1zqrn__nSN-RgPP1yrs8_Tj6_IPdbykLORPmS7G9W1-5ViIQ28DrO9N_loQSy
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Differential+epigenetic+regulation+of+BDNF+and+NT-3+genes+by+trichostatin+A+and+5-aza-2%27-deoxycytidine+in+Neuro-2a+cells&rft.jtitle=Biochemical+and+biophysical+research+communications&rft.au=Ishimaru%2C+Naoki&rft.au=Fukuchi%2C+Mamoru&rft.au=Hirai%2C+Akina&rft.au=Chiba%2C+Yusuke&rft.date=2010-03-26&rft.eissn=1090-2104&rft.volume=394&rft.issue=1&rft.spage=173&rft_id=info:doi/10.1016%2Fj.bbrc.2010.02.139&rft_id=info%3Apmid%2F20188708&rft.externalDocID=20188708
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-291X&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-291X&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-291X&client=summon