Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction

• Published in: Journal of clinical oncology Vol. 12; no. 3; p. 475
• Main Authors: Datta, Y H, Adams, P T, Drobyski, W R, Ethier, S P, Terry, V H, Roth, M S
• Format: Journal Article
• Language: English
• Published: United States 01.03.1994
• Subjects: Base Sequence; Biomarkers, Tumor - analysis; Bone Marrow - metabolism; Breast Neoplasms - diagnosis; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Female; Humans; Keratins - analysis; Keratins - genetics; Molecular Sequence Data; Neoplasm Metastasis - diagnosis; Polymerase Chain Reaction - methods; RNA, Messenger - analysis; RNA, Neoplasm - analysis; RNA-Directed DNA Polymerase; Sensitivity and Specificity

Detection of occult carcinoma in patients with breast cancer may aid the establishment of prognosis and development of new therapeutic approaches. To improve on existing methods of detection, we have developed a reverse-transcriptase polymerase chain reaction (RT-PCR) assay for keratin 19 (K19) tran...