Effect of lactobacillus on the gut microflora and barrier function of the rats with abdominal infection

AIM: TO investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection.METHODS: After the model of cecal ligation and perforation established, SD rats were divided into two groups:parenteral nu...

Full description

Saved in:
Bibliographic Details
Published inWorld journal of gastroenterology : WJG Vol. 11; no. 17; pp. 2591 - 2596
Main Authors Qin, Huan-Long, Shen, Tong-Yi, Gao, Zhi-Guang, Fan, Xiao-Bing, Hang, Xiao-Min, Jiang, Yan-Qun, Zhang, Hui-Zhen
Format Journal Article
LanguageEnglish
Published United States Baishideng Publishing Group Inc 07.05.2005
Subjects
Online AccessGet full text

Cover

Loading…
Abstract AIM: TO investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection.METHODS: After the model of cecal ligation and perforation established, SD rats were divided into two groups:parenteral nutrition (PN) group and PN+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR). The ultrastructure of epithelial light junctions and microvilli of the gut were observed by electron microscopy; ocduding expression was measured by indirect-immune fluorescence method;anaerobic bacterial growth by anaerobic culture and DNA fingerprint of bacterial colonies of the feces by PCR.RESULTS: The quantity of lactobacteria and bifydobacteria in probiotics group was higher than that of PN group. The profiles of DNA fingerprint expression in probiotics group were similar to that in the normal group, a new 16S rDNA sequence appeared in the profile in PN group. The occludin expression, the integrality of the gut epithelial tight junction and microvilli in probiotics group were improved as compared with PN group. The BTR and endotoxin in blood we rereduced more significantly in probiotics group as compared with PN group.CONCLUSION: The probiotics could improve the gut microflora disturbance, increase occludin expression,maintain the gut epithelial tight junction and decrease the bacterial translocations rate.
AbstractList To investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection. After the model of cecal ligation and perforation established, SD rats were divided into two groups: parenteral nutrition (PN) group and PN+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR). The ultra-structure of epithelial tight junctions and microvilli of the gut were observed by electron microscopy; occluding expression was measured by indirect-immune fluorescence method; anaerobic bacterial growth by anaerobic culture and DNA fingerprint of bacterial colonies of the feces by PCR. The quantity of lactobacteria and bifydobacteria in probiotics group was higher than that of PN group. The profiles of DNA fingerprint expression in probiotics group were similar to that in the normal group, a new 16S rDNA sequence appeared in the profile in PN group. The occludin expression, the integrality of the gut epithelial tight junction and microvilli in probiotics group were improved as compared with PN group. The BTR and endotoxin in blood were reduced more significantly in probiotics group as compared with PN group. The probiotics could improve the gut microflora disturbance, increase occludin expression, maintain the gut epithelial tight junction and decrease the bacterial translocations rate.
AIMTo investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection.METHODSAfter the model of cecal ligation and perforation established, SD rats were divided into two groups: parenteral nutrition (PN) group and PN+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR). The ultra-structure of epithelial tight junctions and microvilli of the gut were observed by electron microscopy; occluding expression was measured by indirect-immune fluorescence method; anaerobic bacterial growth by anaerobic culture and DNA fingerprint of bacterial colonies of the feces by PCR.RESULTSThe quantity of lactobacteria and bifydobacteria in probiotics group was higher than that of PN group. The profiles of DNA fingerprint expression in probiotics group were similar to that in the normal group, a new 16S rDNA sequence appeared in the profile in PN group. The occludin expression, the integrality of the gut epithelial tight junction and microvilli in probiotics group were improved as compared with PN group. The BTR and endotoxin in blood were reduced more significantly in probiotics group as compared with PN group.CONCLUSIONThe probiotics could improve the gut microflora disturbance, increase occludin expression, maintain the gut epithelial tight junction and decrease the bacterial translocations rate.
AIM: TO investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection.METHODS: After the model of cecal ligation and perforation established, SD rats were divided into two groups:parenteral nutrition (PN) group and PN+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR). The ultrastructure of epithelial light junctions and microvilli of the gut were observed by electron microscopy; ocduding expression was measured by indirect-immune fluorescence method;anaerobic bacterial growth by anaerobic culture and DNA fingerprint of bacterial colonies of the feces by PCR.RESULTS: The quantity of lactobacteria and bifydobacteria in probiotics group was higher than that of PN group. The profiles of DNA fingerprint expression in probiotics group were similar to that in the normal group, a new 16S rDNA sequence appeared in the profile in PN group. The occludin expression, the integrality of the gut epithelial tight junction and microvilli in probiotics group were improved as compared with PN group. The BTR and endotoxin in blood we rereduced more significantly in probiotics group as compared with PN group.CONCLUSION: The probiotics could improve the gut microflora disturbance, increase occludin expression,maintain the gut epithelial tight junction and decrease the bacterial translocations rate.
AIM: To investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection. METHODS: After the model of cecal ligation and perforation established, SD rats were divided into two groups: parenteral nutrition (PN) group and PN+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR). The ultra-structure of epithelial tight junctions and microvilli of the gut were observed by electron microscopy; occluding expression was measured by indirect-immune fluorescence method; anaerobic bacterial growth by anaerobic culture and DNA fingerprint of bacterial colonies of the feces by PCR. RESULTS: The quantity of lactobacteria and bifydobacteria in probiotics group was higher than that of PN group. The profiles of DNA fingerprint expression in probiotics group were similar to that in the normal group, a new 16S rDNA sequence appeared in the profile in PN group. The occludin expression, the integrality of the gut epithelial tight junction and microvilli in probiotics group were improved as compared with PN group. The BTR and endotoxin in blood were reduced more significantly in probiotics group as compared with PN group. CONCLUSION: The probiotics could improve the gut microflora disturbance, increase occludin expression, maintain the gut epithelial tight junction and decrease the bacterial translocations rate.
Author Huan-LongQin Tong-YiShen Zhi-GuangGao Xiao-BingFan Xiao-MinHang Yan-QunJiang Hui-ZhenZhang
AuthorAffiliation DepartmentofSurgery,ShanghaiJiaoTongUniversityAffiliatedSixthPeople'sHospital,Shanghai200233,China
Author_xml – sequence: 1
  givenname: Huan-Long
  surname: Qin
  fullname: Qin, Huan-Long
  email: sshospy@public.sta.net.cn
  organization: Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China. sshospy@public.sta.net.cn
– sequence: 2
  givenname: Tong-Yi
  surname: Shen
  fullname: Shen, Tong-Yi
– sequence: 3
  givenname: Zhi-Guang
  surname: Gao
  fullname: Gao, Zhi-Guang
– sequence: 4
  givenname: Xiao-Bing
  surname: Fan
  fullname: Fan, Xiao-Bing
– sequence: 5
  givenname: Xiao-Min
  surname: Hang
  fullname: Hang, Xiao-Min
– sequence: 6
  givenname: Yan-Qun
  surname: Jiang
  fullname: Jiang, Yan-Qun
– sequence: 7
  givenname: Hui-Zhen
  surname: Zhang
  fullname: Zhang, Hui-Zhen
BackLink https://www.ncbi.nlm.nih.gov/pubmed/15849817$$D View this record in MEDLINE/PubMed
BookMark eNpVkU1vFSEUhompsbfVvStDXLibKx8zMGxMTFM_kiZudE3OMDCXykALTBv_vdz0xo8VOeF530N4LtBZTNEi9JqSPZf9-P7xdtk_ULr3VO7ZoOgztGOMqo6NPTlDO0qI7BRn8hxdlHJLCON8YC_QOR3GXo1U7tBy7Zw1FSeHA5iaJjA-hK3gFHE9WLxsFa_e5ORCyoAhzniCnL3N2G3RVN-4lj2iGWrBj74eMExzWn2EgH08tjfoJXruIBT76nReoh-frr9ffeluvn3-evXxpjM9o7WbpkEROk_UcDcLoahwzsmZOT5Y0QZgRFrDJFE9E8yBIlI47rgdQTCQkl-iD0-9d9u02tnYWDMEfZf9CvmXTuD1_zfRH_SSHnTPySB71QrenQpyut9sqXr1xdgQINq0FS2klIMYxwaSJ7B9TinZuj9LKNFHO7rZ0c2Obnb00U6LvPn3cX8DJx0NeHvqPKS43Pu46Cbkp_PBNkgIKdjAfwNDGpxW
CitedBy_id crossref_primary_10_3920_BM2017_0172
crossref_primary_10_1111_j_1365_2036_2010_04492_x
crossref_primary_10_1016_j_tjnut_2023_01_026
crossref_primary_10_2165_00124363_200620010_00004
crossref_primary_10_3390_ani11082418
crossref_primary_10_4014_jmb_2209_09026
crossref_primary_10_3390_ijms22136729
crossref_primary_10_1128_IAI_01449_05
crossref_primary_10_3748_wjg_v12_i27_4352
crossref_primary_10_1152_ajpgi_00327_2009
crossref_primary_10_1007_s12602_021_09767_7
crossref_primary_10_1038_sj_ejcn_1602792
crossref_primary_10_1080_21688370_2014_1000707
crossref_primary_10_1016_j_psj_2023_103282
crossref_primary_10_1089_jmf_2010_0040
crossref_primary_10_1016_j_otc_2022_06_003
crossref_primary_10_1007_s11033_014_3701_9
crossref_primary_10_1111_jam_12686
crossref_primary_10_1016_j_eclnm_2009_08_004
crossref_primary_10_2478_aoas_2022_0057
crossref_primary_10_1089_sur_2015_072
crossref_primary_10_3390_microorganisms12050873
crossref_primary_10_1017_S0954422410000260
crossref_primary_10_1016_j_biopha_2020_109883
crossref_primary_10_1097_MEG_0b013e3283556e2b
crossref_primary_10_1111_j_1749_6632_2009_04059_x
crossref_primary_10_3389_fmicb_2023_1288487
crossref_primary_10_2174_0929867329666220415114343
crossref_primary_10_2147_IDR_S345789
crossref_primary_10_3389_fimmu_2023_1195382
crossref_primary_10_1371_journal_ppat_1000879
crossref_primary_10_3920_BM2010_0027
crossref_primary_10_1186_s13099_017_0178_9
crossref_primary_10_1007_s12602_024_10247_x
crossref_primary_10_1111_j_1574_6976_2006_00020_x
crossref_primary_10_1111_j_1574_695X_2007_00264_x
crossref_primary_10_1021_acsomega_3c07529
crossref_primary_10_4161_gmic_1_3_12484
crossref_primary_10_1079_PNS2005466
crossref_primary_10_1097_MOG_0b013e3282f0cffc
crossref_primary_10_1002_ijc_32942
crossref_primary_10_3390_molecules28020619
crossref_primary_10_3390_cells11162617
crossref_primary_10_3389_fmicb_2022_1042263
crossref_primary_10_1007_s40263_016_0370_3
crossref_primary_10_3390_foods13132058
crossref_primary_10_1179_146532806X107403
crossref_primary_10_3390_foods11111567
crossref_primary_10_1007_s13205_021_02796_7
crossref_primary_10_1111_liv_12280
crossref_primary_10_1097_MCO_0b013e32830a70ab
crossref_primary_10_1155_2013_237921
crossref_primary_10_1152_ajpgi_90534_2008
crossref_primary_10_3389_fnut_2024_1385159
crossref_primary_10_1007_s00112_006_1337_0
ContentType Journal Article
Copyright 2005 Baishideng Publishing Group Inc. All rights reserved. 2005
Copyright_xml – notice: 2005 Baishideng Publishing Group Inc. All rights reserved. 2005
DBID 2RA
92L
CQIGP
W91
~WA
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
DOI 10.3748/wjg.v11.i17.2591
DatabaseName 中文科技期刊数据库
中文科技期刊数据库-CALIS站点
中文科技期刊数据库-7.0平台
中文科技期刊数据库-医药卫生
中文科技期刊数据库- 镜像站点
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic


Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
EISSN 2219-2840
EndPage 2596
ExternalDocumentID 10_3748_wjg_v11_i17_2591
15849817
15667625
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
123
29R
2B.
2C~
2RA
2WC
36B
53G
5VR
8WL
92F
92I
92L
93N
93R
AAKDD
ACGFO
AENEX
AFUIB
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CDYEO
CHBEP
CIEJG
CQIGP
CS3
CW9
DIK
DU5
E3Z
EBS
EJD
EMB
F5P
FA0
FRP
GX1
HYE
M~E
OK1
P2P
RNS
RPM
SV3
TCJ
TGQ
TR2
W91
WFFXF
XSB
~WA
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
ID FETCH-LOGICAL-c421t-bb5901db1c3fd66916fff7d2f35e616fa207ec27094262fa9076f3f3e8a62a773
IEDL.DBID RPM
ISSN 1007-9327
IngestDate Fri Sep 01 02:36:01 EDT 2023
Fri Oct 25 09:48:44 EDT 2024
Thu Nov 21 20:42:32 EST 2024
Wed Oct 16 00:47:22 EDT 2024
Thu Nov 24 20:32:51 EST 2022
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed false
IsScholarly true
Issue 17
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c421t-bb5901db1c3fd66916fff7d2f35e616fa207ec27094262fa9076f3f3e8a62a773
Notes R515
14-1219/R
R57
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Correspondence to: Dr. Huan-Long Qin, Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China. sshospy@public.sta.net.cn
Telephone: +86-21-64942226 Fax: +86-21-64368920
Author contributions: All authors contributed equally to the work.
OpenAccessLink https://doi.org/10.3748/wjg.v11.i17.2591
PMID 15849817
PQID 67775688
PQPubID 23479
PageCount 6
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_4305749
proquest_miscellaneous_67775688
crossref_primary_10_3748_wjg_v11_i17_2591
pubmed_primary_15849817
chongqing_backfile_15667625
PublicationCentury 2000
PublicationDate 2005-05-07
PublicationDateYYYYMMDD 2005-05-07
PublicationDate_xml – month: 05
  year: 2005
  text: 2005-05-07
  day: 07
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle World journal of gastroenterology : WJG
PublicationTitleAlternate World Journal of Gastroenterology
PublicationYear 2005
Publisher Baishideng Publishing Group Inc
Publisher_xml – name: Baishideng Publishing Group Inc
SSID ssj0023352
Score 2.1233115
Snippet AIM: TO investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with...
To investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with...
AIMTo investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with...
AIM: To investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with...
SourceID pubmedcentral
proquest
crossref
pubmed
chongqing
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 2591
SubjectTerms Animals
Bacterial Translocation
Basic Research
Intestinal Mucosa - metabolism
Intestines - microbiology
Lactobacillus
Microscopy, Electron
Peritonitis - metabolism
Peritonitis - microbiology
Peritonitis - therapy
Probiotics - pharmacology
Rats
Rats, Sprague-Dawley
Tight Junctions - metabolism
Tight Junctions - microbiology
Tight Junctions - ultrastructure
乳酸菌
内脏系统
动物实验
屏障功能
腹部传染病
Title Effect of lactobacillus on the gut microflora and barrier function of the rats with abdominal infection
URI http://lib.cqvip.com/qk/84123X/200517/15667625.html
https://www.ncbi.nlm.nih.gov/pubmed/15849817
https://search.proquest.com/docview/67775688
https://pubmed.ncbi.nlm.nih.gov/PMC4305749
Volume 11
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwELXaXuCCQOUjFFofuHBItnEcT3JEFVWFVMSBSr1F48ReFnadtrsLf58ZJylduCHlEiWOIr9R3rx4_EaId6ruUBuj0tyBSbXvVIoa81RBjabGuuhij6XLz-biSn-6Lq_3RDnthYlF-61dZGG5ysLiW6ytvFm1s6lObPbl8oxtqkDXs32xT_Q7SfRRZfEmorjEeQopJScwrE2yy8rs1_d59jPPs0UOGaX9sUsMEXBdcbuyR_S5CfNbIoxdivon7_y7fPIBH50_FU_GRFJ-GF74mdhz4VDMBzNi2Xu55EY6FtvFcrldyz5IyvTkfLuRKy7B8yTTUWLopMU7blonmeAYJB7Lt1JkrCX_pZVouz62_pJT5VZ4Lq7OP349u0jHVgppq1W-Sa3lPaadzdvCd8ZQTui9h075onSGTlCdgmsVkNhTRnkkyWx84QtXoVEIULwQB6EP7pWQBQOqkcivKLWycUQNrtJOl1hWmIij-5kkKm5_sMFUwzKRvrtlIt5Pc9vcDH4aDekQhqcheEiz5w3B0zA8iTiZJr-hoOeVDAyu364bAwClqapEvByg-POsEc9EwA5I9zewnfbuFYqyaKs9RtXr_x55JB5HY1c-4I042Nxt3VtKWTb2OIbobwhK7Ew
link.rule.ids 230,314,727,780,784,885,27924,27925,53791,53793
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwELVKOZQLAvEVCtQHLhySrb-TI6qoFuhWHFqpt8hO7GXLrlO6u_D3mXGSwsINKZcodhTNG3nmxeM3hLzlVWul1jxn3uhchpbnVlqWc1NZXdlKtKnH0uxcTy_lpyt1tUfUeBYmFe03blHE5aqIi6-ptvJm1UzGOrHJl9kJylQZWU3ukftKmIqNJH3gWXiMKG1yHpsc0hPT706izsrk5_W8-MFYsWCmgMQ_9YmBEFyV2LDsABacOP8OIWM3SP2Tef5dQPlHRDp9RB4OqSR933_yY7Ln4xMy7-WIaRfoElvpONsslsvtmnaRQq5H59sNXWERXgCibqmNLXX2FtvWUQxxCBPOxaHgG2uK_2mpdW2Xmn_RsXYrPiWXpx8uTqb50EwhbyRnm9w5PGXaOtaI0GoNWWEIwbQ8COU13Fh-bHzDDdA9rnmwQJp1EEH40mpujRHPyH7son9BqEBIpYXwJ5TkLs2ojC-ll8qq0mbk8M6SEIybbygxVSNRhJVXZeTdaNv6plfUqIGJIDw1wAOsndUAT43wZORoNH4Nbo97GTb6bruutTFG6bLMyPMeit_vGvDMiNkB6W4ACmrvPgE_S8Lag1-9_O-ZR-RgejE7q88-nn8-JA-SzCte5hXZ39xu_WtIYDbuTXLXX5yQ76E
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwELWgSMAFFfEV2lIfuHBI0jiOnRyrwqp8tOqBSr1ZdmxvF3adpbsLf58ZJylduCHlEiWOIr9RZl48fo-Qt6yxmgvB0sJJkXJvWaq5LlImGy0a3ZQ2eiydnYvTS_7pqrq6Y_UVm_ZbM8vCfJGF2XXsrVwu2nzsE8svzk5QpkryJl9an98nD6oSgmwk6gPXwq1EcaHzSKZQosh-hRK1VvJf36bZz6LIZoXMoPiPXjGQhpsaTcsewUcnTH9A2thOVP9Un383Ud7JSpNd8mQoJ-lx_9pPyT0XnpFpL0lMO0_naKdjdDubzzcr2gUK9R6dbtZ0gY14Hsi6pjpYavQNWtdRTHMIFY7FWyE-VhT_1VJtbBcNwOjYvxWek8vJh68np-lgqJC2nBXr1BjcaWpN0ZbeCgGVofdeWubLygk40exIupZJoHxMMK-BOAtf-tLVWjAtZfmC7IQuuFeElggr15ACy4ozE0c00tXc8UpXtU7I3u1MQkJuv6PMlEKyCF_fKiHvxrlVy15VQwEbQXgUwAPMvVAAj0J4EnI4Tr6C0Mf1DB1ct1kpIaWsRF0n5GUPxZ9nDXgmRG6BdHsDimpvX4FYi-LaQ2y9_u-Rh-ThxfuJ-vLx_PMeeRyVXvGQ-2RnfbNxB1DDrM2bGK2_ARFD8LQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Effect+of+lactobacillus+on+the+gut+microflora+and+barrier+function+of+the+rats+with+abdominal+infection&rft.jtitle=World+journal+of+gastroenterology+%3A+WJG&rft.au=Qin%2C+Huan-Long&rft.date=2005-05-07&rft.issn=1007-9327&rft.volume=11&rft.issue=17&rft.spage=2591&rft_id=info:doi/10.3748%2Fwjg.v11.i17.2591&rft.externalDBID=n%2Fa&rft.externalDocID=10_3748_wjg_v11_i17_2591
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F84123X%2F84123X.jpg