Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression

• Published in: Journal of biotechnology Vol. 158; no. 1-2; pp. 59 - 67
• Main Authors: Castro-Martínez, Claudia, Luna-Suárez, Silvia, Paredes-López, Octavio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.03.2012
• Subjects: 11S acidic subunit; ACE inhibitory activity; agitation; air flow; amaranth grain; Amaranth seed; Amaranthus - genetics; amino acid composition; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Antihypertensive peptides; Bioreactors; culture media; dietary protein; environmental factors; Escherichia coli; Escherichia coli - genetics; functional foods; Gene Expression Regulation, Plant; globulins; hydrolysis; oxygen; peptides; Peptides - chemistry; peptidyl-dipeptidase A; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - isolation & purification; protein synthesis; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Seeds - genetics; temperature; 11S acidic subunit; ACE inhibitory activity; Amaranth seed; Antihypertensive peptides

► We inserted 4 VY and 1 IPP antihypertensive peptides in the amarantin acidic subunit. ► Modified protein was overexpressed in Escherichia coli in a fermentor finding the best factors. ► The temperature, agitation speed and oxygen had effect on the yield of the protein. ► Modified protein is ten ti...