Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression

• Published in: Journal of biotechnology Vol. 158; no. 1-2; pp. 59 - 67
• Main Authors: Castro-Martínez, Claudia, Luna-Suárez, Silvia, Paredes-López, Octavio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.03.2012
• Subjects: 11S acidic subunit; ACE inhibitory activity; agitation; air flow; amaranth grain; Amaranth seed; Amaranthus - genetics; amino acid composition; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Antihypertensive peptides; Bioreactors; culture media; dietary protein; environmental factors; Escherichia coli; Escherichia coli - genetics; functional foods; Gene Expression Regulation, Plant; globulins; hydrolysis; oxygen; peptides; Peptides - chemistry; peptidyl-dipeptidase A; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - isolation & purification; protein synthesis; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Seeds - genetics; temperature; 11S acidic subunit; ACE inhibitory activity; Amaranth seed; Antihypertensive peptides
• ISSN: 0168-1656; 1873-4863; 1873-4863
• DOI: 10.1016/j.jbiotec.2011.12.012

► We inserted 4 VY and 1 IPP antihypertensive peptides in the amarantin acidic subunit. ► Modified protein was overexpressed in Escherichia coli in a fermentor finding the best factors. ► The temperature, agitation speed and oxygen had effect on the yield of the protein. ► Modified protein is ten times more active than the non-modified sample. Amaranth seeds are considered as an excellent complementary source of food protein due to their balanced amino acid composition. Amarantin acidic subunit has the potential as a functional and nutraceutical protein, and it is structurally a good candidate for modification. The aim of this work was to improve its functionality, then the primary structure was modified into the third variable region of 11S globulins, by inserting antihypertensive peptides: four Val-Tyr in tandem and Arg-Ile-Pro-Pro in the C-terminal region. Modified protein was expressed in Escherichia coli Origami (DE3) and was purified. The culture conditions, including the culture media, temperature, agitation speed and air flow were tested in order to obtain an increased expression levels of the modified protein. A 23 factorial design was used for evaluate the effect of environmental conditions on modified protein production. The results indicated that the yield of modified protein could be increased by up 3-fold in bioreactor as compared with flask. In addition, the temperature, the agitation speed and the oxygen were significant factors on the expression of the antihypertensive protein. The maximum production was 99mg protein-L−1. The hydrolyzed protein showed a high inhibitory activity of the angiotensin converting enzyme (IC50=0.047mgmL−1).