Single-spore germination analyses reveal that calcium released during Clostridioides difficile germination functions in a feedforward loop

• Published in: mSphere Vol. 8; no. 4; p. e0000523
• Main Authors: Ribis, John W, Melo, Luana, Shrestha, Shailab, Giacalone, David, Rodriguez, Enrique E, Shen, Aimee, Rohlfing, Amy
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 24.08.2023
• Subjects: Amino acids; Amino Acids - metabolism; Antibiotics; automated image analysis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bile; Bile Acids and Salts - metabolism; Bile Acids and Salts - pharmacology; Calcium - metabolism; Calcium signalling; Clostridioides - metabolism; Clostridioides difficile; Clostridioides difficile - physiology; co-germinant; DPA; Enzymes; Image processing; Microscopy; Mutants; Mutation; Nosocomial infections; Optical density; Proteins; Small intestine; Spore germination; Spores, Bacterial - physiology; time-lapse microscopy; spore germination; Clostridioides difficile; automated image analysis; DPA; time-lapse microscopy; co-germinant

infections begin when its metabolically dormant spores germinate in response to sensing bile acid germinants alongside amino acid and divalent cation co-germinants in the small intestine. While bile acid germinants are essential for spore germination, it is currently unclear whether both co-germinan...