Pre-radiation enhances the cytotoxicity of docetaxel in head and neck squamous cell carcinoma cells

• Published in: Oncology reports Vol. 23; no. 5; pp. 1339 - 1343
• Main Authors: FURUSE, Satori, ADACHI, Makoto, IJICHI, Kei, OHTA, Shinsuke, TORIGOE, Soichiro, NAKAZAWA, Masatoshi, MIURA, Satoshi, MITSUDO, Kenji, TOHNAI, Iwai
• Format: Journal Article
• Language: English
• Published: Athens Spandidos 01.05.2010
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Biological and medical sciences; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Cell Cycle - drug effects; Cell Cycle - radiation effects; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Proliferation - radiation effects; Cell Survival - drug effects; Cell Survival - radiation effects; Chemotherapy, Adjuvant; Docetaxel; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - pathology; Humans; Medical sciences; Mutation; Otorhinolaryngology (head neck, general aspects and miscellaneous); Otorhinolaryngology. Stomatology; Radiotherapy, Adjuvant; Taxoids - pharmacology; Time Factors; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Tumors; Antineoplastic agent; Docetaxel; Cytotoxicity; Malignant tumor; Cancerology; radiation; Taxane derivatives; Cell cycle; ENT disease; Head and neck cancer; Head and neck squamous cell carcinoma; combination therapy; Combined treatment; Antimitotic; Cancer
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or_00000769

This study was designed to determine the effect of the treatment schedule on the interaction between docetaxel and irradiation. Human head and neck squamous cell carcinoma (HNSCC) cells with different p53 status, and HSC4 (p53 wild-type) and CAL27 (p53 mutant type) cells were treated with docetaxel and irradiation using three schedules: i) concurrent treatment, ii) docetaxel pretreatment and iii) pre-radiation. Docetaxel and radiation inhibited the proliferation of HSC4 and CAL27 cells in a dose-dependent manner. However, irradiation pretreatment was more effective than the other treatment regimens in all cells. Our data suggest that pre-radiation in HNSCC cells significantly enhances docetaxel cytotoxity by arresting S-phase, and this provides the most effective treatment sequence of docetaxel and radiation combination therapy. Therefore, radiation followed by docetaxel may be the most effective sequence for head and neck cancer therapy.