Skin-resident dendritic cells mediate postoperative pain via CCR4 on sensory neurons

Inflammatory pain, such as hypersensitivity resulting from surgical tissue injury, occurs as a result of interactions between the immune and nervous systems with the orchestrated recruitment and activation of tissue-resident and circulating immune cells to the site of injury. Our previous studies id...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 119; no. 4; p. 1
Main Authors Silva, Jaqueline Raymondi, Iftinca, Mircea, Fernandes Gomes, Francisco I, Segal, Julia P, Smith, Olivia M A, Bannerman, Courtney A, Silva Mendes, Atlante, Defaye, Manon, Robinson, Madeline E C, Gilron, Ian, Cunha, Thiago Mattar, Altier, Christophe, Ghasemlou, Nader
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 25.01.2022
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Inflammatory pain, such as hypersensitivity resulting from surgical tissue injury, occurs as a result of interactions between the immune and nervous systems with the orchestrated recruitment and activation of tissue-resident and circulating immune cells to the site of injury. Our previous studies identified a central role for Ly6C myeloid cells in the pathogenesis of postoperative pain. We now show that the chemokines CCL17 and CCL22, with their cognate receptor CCR4, are key mediators of this response. Both chemokines are up-regulated early after tissue injury by skin-resident dendritic and Langerhans cells to act on peripheral sensory neurons that express CCR4. CCL22, and to a lesser extent CCL17, elicit acute mechanical and thermal hypersensitivity when administered subcutaneously; this response abrogated by pharmacological blockade or genetic silencing of CCR4. Electrophysiological assessment of dissociated sensory neurons from naïve and postoperative mice showed that CCL22 was able to directly activate neurons and enhance their excitability after injury. These responses were blocked using C 021 and small interfering RNA (siRNA)-targeting CCR4. Finally, our data show that acute postoperative pain is significantly reduced in mice lacking CCR4, wild-type animals treated with CCR4 antagonist/siRNA, as well as transgenic mice depleted of dendritic cells. Together, these results suggest an essential role for the peripheral CCL17/22:CCR4 axis in the genesis of inflammatory pain via direct communication between skin-resident dendritic cells and sensory neurons, opening therapeutic avenues for its control.
AbstractList Interactions between the nervous and immune systems control the generation and maintenance of inflammatory pain. However, the immune cells and mediators controlling this response remain poorly characterized. We identified the cytokines CCL22 and CCL17 as secreted mediators that act directly on sensory neurons to mediate postoperative pain via their shared receptor, CCR4. We also show that skin-resident dendritic cells are key contributors to the inflammatory pain response. Blocking the interaction between these dendritic cell–derived ligands and their receptor can abrogate the pain response, highlighting CCR4 antagonists as potentially effective therapies for postoperative pain. Our findings identify functions for these tissue-resident myeloid cells and uncover mechanisms underlying pain pathophysiology. Inflammatory pain, such as hypersensitivity resulting from surgical tissue injury, occurs as a result of interactions between the immune and nervous systems with the orchestrated recruitment and activation of tissue-resident and circulating immune cells to the site of injury. Our previous studies identified a central role for Ly6C low myeloid cells in the pathogenesis of postoperative pain. We now show that the chemokines CCL17 and CCL22, with their cognate receptor CCR4, are key mediators of this response. Both chemokines are up-regulated early after tissue injury by skin-resident dendritic and Langerhans cells to act on peripheral sensory neurons that express CCR4. CCL22, and to a lesser extent CCL17, elicit acute mechanical and thermal hypersensitivity when administered subcutaneously; this response abrogated by pharmacological blockade or genetic silencing of CCR4. Electrophysiological assessment of dissociated sensory neurons from naïve and postoperative mice showed that CCL22 was able to directly activate neurons and enhance their excitability after injury. These responses were blocked using C 021 and small interfering RNA (siRNA)-targeting CCR4. Finally, our data show that acute postoperative pain is significantly reduced in mice lacking CCR4, wild-type animals treated with CCR4 antagonist/siRNA, as well as transgenic mice depleted of dendritic cells. Together, these results suggest an essential role for the peripheral CCL17/22:CCR4 axis in the genesis of inflammatory pain via direct communication between skin-resident dendritic cells and sensory neurons, opening therapeutic avenues for its control.
Inflammatory pain, such as hypersensitivity resulting from surgical tissue injury, occurs as a result of interactions between the immune and nervous systems with the orchestrated recruitment and activation of tissue-resident and circulating immune cells to the site of injury. Our previous studies identified a central role for Ly6C myeloid cells in the pathogenesis of postoperative pain. We now show that the chemokines CCL17 and CCL22, with their cognate receptor CCR4, are key mediators of this response. Both chemokines are up-regulated early after tissue injury by skin-resident dendritic and Langerhans cells to act on peripheral sensory neurons that express CCR4. CCL22, and to a lesser extent CCL17, elicit acute mechanical and thermal hypersensitivity when administered subcutaneously; this response abrogated by pharmacological blockade or genetic silencing of CCR4. Electrophysiological assessment of dissociated sensory neurons from naïve and postoperative mice showed that CCL22 was able to directly activate neurons and enhance their excitability after injury. These responses were blocked using C 021 and small interfering RNA (siRNA)-targeting CCR4. Finally, our data show that acute postoperative pain is significantly reduced in mice lacking CCR4, wild-type animals treated with CCR4 antagonist/siRNA, as well as transgenic mice depleted of dendritic cells. Together, these results suggest an essential role for the peripheral CCL17/22:CCR4 axis in the genesis of inflammatory pain via direct communication between skin-resident dendritic cells and sensory neurons, opening therapeutic avenues for its control.
Inflammatory pain, such as hypersensitivity resulting from surgical tissue injury, occurs as a result of interactions between the immune and nervous systems with the orchestrated recruitment and activation of tissue-resident and circulating immune cells to the site of injury. Our previous studies identified a central role for Ly6Clow myeloid cells in the pathogenesis of postoperative pain. We now show that the chemokines CCL17 and CCL22, with their cognate receptor CCR4, are key mediators of this response. Both chemokines are up-regulated early after tissue injury by skin-resident dendritic and Langerhans cells to act on peripheral sensory neurons that express CCR4. CCL22, and to a lesser extent CCL17, elicit acute mechanical and thermal hypersensitivity when administered subcutaneously; this response abrogated by pharmacological blockade or genetic silencing of CCR4. Electrophysiological assessment of dissociated sensory neurons from naïve and postoperative mice showed that CCL22 was able to directly activate neurons and enhance their excitability after injury. These responses were blocked using C 021 and small interfering RNA (siRNA)-targeting CCR4. Finally, our data show that acute postoperative pain is significantly reduced in mice lacking CCR4, wild-type animals treated with CCR4 antagonist/siRNA, as well as transgenic mice depleted of dendritic cells. Together, these results suggest an essential role for the peripheral CCL17/22:CCR4 axis in the genesis of inflammatory pain via direct communication between skin-resident dendritic cells and sensory neurons, opening therapeutic avenues for its control.
Author Silva, Jaqueline Raymondi
Silva Mendes, Atlante
Segal, Julia P
Defaye, Manon
Robinson, Madeline E C
Cunha, Thiago Mattar
Fernandes Gomes, Francisco I
Gilron, Ian
Altier, Christophe
Iftinca, Mircea
Ghasemlou, Nader
Smith, Olivia M A
Bannerman, Courtney A
Author_xml – sequence: 1
  givenname: Jaqueline Raymondi
  orcidid: 0000-0001-8961-3572
  surname: Silva
  fullname: Silva, Jaqueline Raymondi
  organization: Department of Anesthesiology and Perioperative Medicine, Queen's University, Kingston, ON K7L 3N6, Canada
– sequence: 2
  givenname: Mircea
  surname: Iftinca
  fullname: Iftinca, Mircea
  organization: Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
– sequence: 3
  givenname: Francisco I
  surname: Fernandes Gomes
  fullname: Fernandes Gomes, Francisco I
  organization: Center for Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, São Paulo 1409-900, Brazil
– sequence: 4
  givenname: Julia P
  surname: Segal
  fullname: Segal, Julia P
  organization: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada
– sequence: 5
  givenname: Olivia M A
  surname: Smith
  fullname: Smith, Olivia M A
  organization: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada
– sequence: 6
  givenname: Courtney A
  surname: Bannerman
  fullname: Bannerman, Courtney A
  organization: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada
– sequence: 7
  givenname: Atlante
  surname: Silva Mendes
  fullname: Silva Mendes, Atlante
  organization: Center for Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, São Paulo 1409-900, Brazil
– sequence: 8
  givenname: Manon
  surname: Defaye
  fullname: Defaye, Manon
  organization: Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
– sequence: 9
  givenname: Madeline E C
  orcidid: 0000-0002-4914-4940
  surname: Robinson
  fullname: Robinson, Madeline E C
  organization: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada
– sequence: 10
  givenname: Ian
  orcidid: 0000-0002-5293-8792
  surname: Gilron
  fullname: Gilron, Ian
  organization: School of Policy Studies, Queen's University, Kingston, ON K7L 3N6, Canada
– sequence: 11
  givenname: Thiago Mattar
  surname: Cunha
  fullname: Cunha, Thiago Mattar
  organization: Center for Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, São Paulo 1409-900, Brazil
– sequence: 12
  givenname: Christophe
  orcidid: 0000-0003-2209-6536
  surname: Altier
  fullname: Altier, Christophe
  organization: Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
– sequence: 13
  givenname: Nader
  orcidid: 0000-0002-1696-7342
  surname: Ghasemlou
  fullname: Ghasemlou, Nader
  email: nader.ghasemlou@queensu.ca
  organization: Centre for Neuroscience Studies, Queen's University, Kingston, ON K7L 3N6, Canada
BackLink https://www.ncbi.nlm.nih.gov/pubmed/35046040$$D View this record in MEDLINE/PubMed
BookMark eNpdkctrGzEQxkVwqe2059yKIJdcNhk9Vo9LIZg0CQQKbXoW2l1tosSWttKuwf99ZZxH28sMw_zmYz6-JZqFGBxCJwTOCUh2MQSbzykhijJFiD5CCwKaVIJrmKEFAJWV4pTP0TLnJwDQtYKPaM5q4AI4LND9z2cfquSy71wYcSld8qNvcevW64w3rvN2dHiIeYyDS3b02zJZH_DWW7xa_eA4BpxdyDHtcHBTiiF_Qh96u87u80s_Rr--Xd2vbqq779e3q8u7quWUjJVUrGuErEH3TiopCYO6dTUF2zOtasY1b7qm6QTvO9oSwSzvpXW0aynphVLsGH096A5TUz5ti4Nk12ZIfmPTzkTrzb-b4B_NQ9waJTVXmheBsxeBFH9PLo9m4_PeuQ0uTtlQQYmotYS6oKf_oU9xSqHY21NcKMEZKdTFgWpTzDm5_u0ZAmafmNknZt4TKxdf_vbwxr9GxP4AR2CUjw
CitedBy_id crossref_primary_10_3390_ijms25073788
crossref_primary_10_1016_j_intimp_2023_110078
crossref_primary_10_1084_jem_20221687
crossref_primary_10_3390_ijms232415638
crossref_primary_10_3389_fphar_2023_1297931
crossref_primary_10_1172_JCI153805
crossref_primary_10_1186_s12974_022_02615_7
crossref_primary_10_3390_antib13020032
crossref_primary_10_1111_imcb_12644
crossref_primary_10_1097_j_pain_0000000000003106
crossref_primary_10_1111_jnc_15724
crossref_primary_10_1093_humrep_dead099
crossref_primary_10_3389_fpain_2023_1154597
Cites_doi 10.1172/jci.insight.99249
10.1111/j.1398-9995.2009.02095.x
10.1152/jn.2001.85.2.644
10.1016/j.immuni.2005.10.008
10.1097/PR9.0000000000000588
10.1523/JNEUROSCI.1650-20.2020
10.1038/nrn2533
10.1189/jlb.2MR0815-392R
10.1200/JCO.2013.52.0924
10.1186/1744-8069-8-18
10.3389/fimmu.2020.01241
10.1152/jn.00065.2011
10.1038/nature13199
10.1371/journal.pone.0074019
10.1016/S0140-6736(06)68700-X
10.1038/nrrheum.2010.175
10.1523/JNEUROSCI.21-14-05027.2001
10.1097/j.pain.0000000000001866
10.1038/s41467-021-21725-z
10.1016/j.bbi.2006.10.013
10.1093/brain/awz063
10.1016/j.it.2016.10.001
10.1038/nrd4334
10.1016/S0304-3959(99)00262-6
10.4049/jimmunol.176.7.4431
10.7554/eLife.04660
10.1038/srep16713
10.1016/0304-3959(95)01441-1
10.1213/01.ANE.0000158471.41575.F0
10.1517/13543770802641346
10.1016/j.pain.2004.08.029
10.1038/nn.3881
10.1186/2050-6511-14-14
10.1038/s41577-019-0147-2
10.1038/ncomms13581
10.1523/JNEUROSCI.2233-16.2017
10.1007/978-1-60761-421-0_28
10.1002/eji.200737485
10.4049/jimmunol.1001802
10.1038/ni1008-1091
10.1016/j.neuroscience.2004.08.035
10.4049/jimmunol.1300232
10.1002/jnr.23831
10.1172/JCI87828
10.1186/s13075-018-1560-9
10.1111/j.0022-202X.2004.23607.x
10.1096/fj.202100319RR
10.1038/s41598-020-72285-z
10.1615/CritRevImmunol.v25.i2.10
10.1523/JNEUROSCI.23-06-02069.2003
10.1016/j.immuni.2015.08.016
10.1038/jid.2009.429
10.1189/jlb.0907654
10.3389/fimmu.2019.00473
10.1016/j.pain.2014.08.025
10.1016/S0306-4522(02)00065-9
10.1016/j.ejphar.2014.02.007
10.1002/eji.200838659
10.1517/17460441.2014.897324
10.1016/j.immuni.2019.02.023
10.1097/ALN.0b013e3182655f9f
10.1186/1744-8069-4-63
10.1016/S0304-3959(01)00433-X
10.1073/pnas.1501372112
10.1111/1346-8138.12440
10.4049/jimmunol.164.8.4048
10.1097/j.pain.0000000000001217
10.1016/j.cyto.2018.11.030
10.1016/j.jdermsci.2006.06.002
10.1038/nri3683
10.1002/eji.200324429
10.1084/jem.187.1.129
10.1084/jem.194.6.847
10.1111/j.1399-3038.2011.01225.x
10.1016/S0079-6123(00)29002-X
10.4049/jimmunol.176.7.4331
10.1111/j.1460-9568.2004.03514.x
ContentType Journal Article
Copyright Copyright © 2022 the Author(s). Published by PNAS.
Copyright National Academy of Sciences Jan 25, 2022
Copyright © 2022 the Author(s). Published by PNAS. 2022
Copyright_xml – notice: Copyright © 2022 the Author(s). Published by PNAS.
– notice: Copyright National Academy of Sciences Jan 25, 2022
– notice: Copyright © 2022 the Author(s). Published by PNAS. 2022
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7QG
7QL
7QP
7QR
7SN
7SS
7T5
7TK
7TM
7TO
7U9
8FD
C1K
FR3
H94
M7N
P64
RC3
7X8
5PM
DOI 10.1073/pnas.2118238119
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Animal Behavior Abstracts
Bacteriology Abstracts (Microbiology B)
Calcium & Calcified Tissue Abstracts
Chemoreception Abstracts
Ecology Abstracts
Entomology Abstracts (Full archive)
Immunology Abstracts
Neurosciences Abstracts
Nucleic Acids Abstracts
Oncogenes and Growth Factors Abstracts
Virology and AIDS Abstracts
Technology Research Database
Environmental Sciences and Pollution Management
Engineering Research Database
AIDS and Cancer Research Abstracts
Algology Mycology and Protozoology Abstracts (Microbiology C)
Biotechnology and BioEngineering Abstracts
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Virology and AIDS Abstracts
Oncogenes and Growth Factors Abstracts
Technology Research Database
Nucleic Acids Abstracts
Ecology Abstracts
Neurosciences Abstracts
Biotechnology and BioEngineering Abstracts
Environmental Sciences and Pollution Management
Entomology Abstracts
Genetics Abstracts
Animal Behavior Abstracts
Bacteriology Abstracts (Microbiology B)
Algology Mycology and Protozoology Abstracts (Microbiology C)
AIDS and Cancer Research Abstracts
Chemoreception Abstracts
Immunology Abstracts
Engineering Research Database
Calcium & Calcified Tissue Abstracts
MEDLINE - Academic
DatabaseTitleList CrossRef
MEDLINE

Virology and AIDS Abstracts
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Sciences (General)
EISSN 1091-6490
ExternalDocumentID 10_1073_pnas_2118238119
35046040
Genre Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: CIHR
  grantid: PJT173288
– fundername: CIHR
  grantid: PJT148980
– fundername: CIHR
  grantid: PJT156418
– fundername: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  grantid: 2013/08216-2-CRID
– fundername: Gouvernement du Canada | Canadian Institutes of Health Research (CIHR)
  grantid: PJT173288
– fundername: Canada Research Chairs (Chaires de recherche du Canada)
  grantid: Inflammatory Pain
– fundername: Queen''''s University (Queen''''s University, Canada)
  grantid: Research Leaders'''' Fund Postdoctoral Fellowship
– fundername: Alberta Children''''s Hospital Foundation
  grantid: Fellowship
– fundername: Gouvernement du Canada | Canadian Institutes of Health Research (CIHR)
  grantid: PJT148980
– fundername: Gouvernement du Canada | Canadian Institutes of Health Research (CIHR)
  grantid: PJT156418
GroupedDBID ---
-DZ
-~X
.55
0R~
123
29P
2FS
2WC
4.4
53G
5RE
5VS
85S
AACGO
AAFWJ
AANCE
ABOCM
ABPLY
ABPPZ
ABTLG
ABZEH
ACGOD
ACIWK
ACNCT
ACPRK
AENEX
AFFNX
AFOSN
AFRAH
ALMA_UNASSIGNED_HOLDINGS
BKOMP
CGR
CS3
CUY
CVF
D0L
DIK
DU5
E3Z
EBS
ECM
EIF
F5P
FRP
GX1
H13
HH5
HYE
JLS
JSG
JST
KQ8
L7B
LU7
N9A
NPM
N~3
O9-
OK1
PNE
PQQKQ
R.V
RHF
RHI
RNA
RNS
RPM
RXW
SJN
TAE
TN5
UKR
VQA
W8F
WH7
WOQ
WOW
X7M
XSW
Y6R
YBH
YKV
YSK
ZCA
~02
~KM
AAYXX
CITATION
7QG
7QL
7QP
7QR
7SN
7SS
7T5
7TK
7TM
7TO
7U9
8FD
C1K
FR3
H94
M7N
P64
RC3
7X8
5PM
ID FETCH-LOGICAL-c421t-783db67509fe78771305ce520af39853494bdbbd64fd2c163a4f7ae2dc21f6883
IEDL.DBID RPM
ISSN 0027-8424
IngestDate Tue Sep 17 21:17:27 EDT 2024
Sat Aug 17 00:41:36 EDT 2024
Thu Oct 10 19:09:03 EDT 2024
Fri Dec 06 02:30:56 EST 2024
Sat Sep 28 08:17:35 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords pain
cytokines
myeloid cells
electrophysiology
inflammation
Language English
License Copyright © 2022 the Author(s). Published by PNAS.
This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c421t-783db67509fe78771305ce520af39853494bdbbd64fd2c163a4f7ae2dc21f6883
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by Allan Basbaum, Department of Anatomy, University of California, San Francisco, CA; received October 15, 2021; accepted December 1, 2021
Author contributions: J.R.S., M.I., T.M.C., C.A., and N.G. designed research; J.R.S., M.I., F.I.F.G., J.P.S., O.M.A.S., C.A.B., A.S.M., M.D., M.E.C.R., and N.G. performed research; J.R.S., M.I., F.I.F.G., J.P.S., O.M.A.S., C.A.B., A.S.M., M.D., M.E.C.R., I.G., T.M.C., C.A., and N.G. analyzed data; and J.R.S., M.I., I.G., T.M.C., C.A., and N.G. wrote the paper.
ORCID 0000-0001-8961-3572
0000-0002-5293-8792
0000-0003-2209-6536
0000-0002-1696-7342
0000-0002-4914-4940
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794894/
PMID 35046040
PQID 2624686431
PQPubID 42026
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_8794894
proquest_miscellaneous_2621659705
proquest_journals_2624686431
crossref_primary_10_1073_pnas_2118238119
pubmed_primary_35046040
PublicationCentury 2000
PublicationDate 2022-01-25
PublicationDateYYYYMMDD 2022-01-25
PublicationDate_xml – month: 01
  year: 2022
  text: 2022-01-25
  day: 25
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Washington
PublicationTitle Proceedings of the National Academy of Sciences - PNAS
PublicationTitleAlternate Proc Natl Acad Sci U S A
PublicationYear 2022
Publisher National Academy of Sciences
Publisher_xml – name: National Academy of Sciences
References e_1_3_4_3_2
e_1_3_4_1_2
e_1_3_4_61_2
e_1_3_4_9_2
e_1_3_4_63_2
e_1_3_4_7_2
e_1_3_4_40_2
e_1_3_4_5_2
e_1_3_4_23_2
e_1_3_4_44_2
e_1_3_4_69_2
e_1_3_4_21_2
e_1_3_4_42_2
e_1_3_4_27_2
e_1_3_4_48_2
e_1_3_4_65_2
e_1_3_4_25_2
e_1_3_4_46_2
e_1_3_4_67_2
e_1_3_4_29_2
e_1_3_4_72_2
e_1_3_4_74_2
e_1_3_4_30_2
e_1_3_4_51_2
e_1_3_4_70_2
e_1_3_4_11_2
e_1_3_4_34_2
e_1_3_4_57_2
e_1_3_4_55_2
e_1_3_4_32_2
e_1_3_4_59_2
e_1_3_4_53_2
e_1_3_4_15_2
e_1_3_4_38_2
e_1_3_4_76_2
e_1_3_4_13_2
e_1_3_4_36_2
e_1_3_4_19_2
e_1_3_4_17_2
e_1_3_4_2_2
e_1_3_4_60_2
e_1_3_4_62_2
e_1_3_4_8_2
e_1_3_4_41_2
e_1_3_4_6_2
e_1_3_4_4_2
e_1_3_4_22_2
e_1_3_4_45_2
e_1_3_4_68_2
e_1_3_4_20_2
e_1_3_4_43_2
e_1_3_4_26_2
e_1_3_4_49_2
e_1_3_4_64_2
e_1_3_4_24_2
e_1_3_4_47_2
e_1_3_4_66_2
e_1_3_4_28_2
e_1_3_4_71_2
e_1_3_4_73_2
e_1_3_4_52_2
e_1_3_4_50_2
e_1_3_4_12_2
e_1_3_4_33_2
e_1_3_4_58_2
e_1_3_4_54_2
e_1_3_4_10_2
e_1_3_4_31_2
e_1_3_4_75_2
e_1_3_4_16_2
e_1_3_4_37_2
e_1_3_4_77_2
e_1_3_4_14_2
e_1_3_4_35_2
e_1_3_4_56_2
e_1_3_4_18_2
e_1_3_4_39_2
References_xml – ident: e_1_3_4_19_2
  doi: 10.1172/jci.insight.99249
– ident: e_1_3_4_40_2
  doi: 10.1111/j.1398-9995.2009.02095.x
– ident: e_1_3_4_64_2
  doi: 10.1152/jn.2001.85.2.644
– ident: e_1_3_4_69_2
  doi: 10.1016/j.immuni.2005.10.008
– ident: e_1_3_4_2_2
  doi: 10.1097/PR9.0000000000000588
– ident: e_1_3_4_12_2
  doi: 10.1523/JNEUROSCI.1650-20.2020
– ident: e_1_3_4_7_2
  doi: 10.1038/nrn2533
– ident: e_1_3_4_46_2
  doi: 10.1189/jlb.2MR0815-392R
– ident: e_1_3_4_75_2
  doi: 10.1200/JCO.2013.52.0924
– ident: e_1_3_4_29_2
  doi: 10.1186/1744-8069-8-18
– ident: e_1_3_4_22_2
  doi: 10.3389/fimmu.2020.01241
– ident: e_1_3_4_61_2
  doi: 10.1152/jn.00065.2011
– ident: e_1_3_4_71_2
  doi: 10.1038/nature13199
– ident: e_1_3_4_49_2
  doi: 10.1371/journal.pone.0074019
– ident: e_1_3_4_1_2
  doi: 10.1016/S0140-6736(06)68700-X
– ident: e_1_3_4_33_2
  doi: 10.1038/nrrheum.2010.175
– ident: e_1_3_4_42_2
  doi: 10.1523/JNEUROSCI.21-14-05027.2001
– ident: e_1_3_4_57_2
  doi: 10.1097/j.pain.0000000000001866
– ident: e_1_3_4_56_2
  doi: 10.1038/s41467-021-21725-z
– ident: e_1_3_4_5_2
  doi: 10.1016/j.bbi.2006.10.013
– ident: e_1_3_4_58_2
  doi: 10.1093/brain/awz063
– ident: e_1_3_4_9_2
  doi: 10.1016/j.it.2016.10.001
– ident: e_1_3_4_10_2
  doi: 10.1038/nrd4334
– ident: e_1_3_4_43_2
  doi: 10.1016/S0304-3959(99)00262-6
– ident: e_1_3_4_52_2
  doi: 10.4049/jimmunol.176.7.4431
– ident: e_1_3_4_26_2
  doi: 10.7554/eLife.04660
– ident: e_1_3_4_60_2
  doi: 10.1038/srep16713
– ident: e_1_3_4_76_2
  doi: 10.1016/0304-3959(95)01441-1
– ident: e_1_3_4_77_2
  doi: 10.1213/01.ANE.0000158471.41575.F0
– ident: e_1_3_4_72_2
  doi: 10.1517/13543770802641346
– ident: e_1_3_4_31_2
  doi: 10.1016/j.pain.2004.08.029
– ident: e_1_3_4_53_2
  doi: 10.1038/nn.3881
– ident: e_1_3_4_74_2
  doi: 10.1186/2050-6511-14-14
– ident: e_1_3_4_11_2
  doi: 10.1038/s41577-019-0147-2
– ident: e_1_3_4_17_2
  doi: 10.1038/ncomms13581
– ident: e_1_3_4_34_2
  doi: 10.1523/JNEUROSCI.2233-16.2017
– ident: e_1_3_4_67_2
  doi: 10.1007/978-1-60761-421-0_28
– ident: e_1_3_4_3_2
  doi: 10.1002/eji.200737485
– ident: e_1_3_4_70_2
  doi: 10.4049/jimmunol.1001802
– ident: e_1_3_4_24_2
  doi: 10.1038/ni1008-1091
– ident: e_1_3_4_35_2
  doi: 10.1016/j.neuroscience.2004.08.035
– ident: e_1_3_4_28_2
  doi: 10.4049/jimmunol.1300232
– ident: e_1_3_4_37_2
  doi: 10.1002/jnr.23831
– ident: e_1_3_4_18_2
  doi: 10.1172/JCI87828
– ident: e_1_3_4_20_2
  doi: 10.1186/s13075-018-1560-9
– ident: e_1_3_4_15_2
  doi: 10.1111/j.0022-202X.2004.23607.x
– ident: e_1_3_4_36_2
  doi: 10.1096/fj.202100319RR
– ident: e_1_3_4_54_2
  doi: 10.1038/s41598-020-72285-z
– ident: e_1_3_4_38_2
  doi: 10.1615/CritRevImmunol.v25.i2.10
– ident: e_1_3_4_62_2
  doi: 10.1523/JNEUROSCI.23-06-02069.2003
– ident: e_1_3_4_16_2
  doi: 10.1016/j.immuni.2015.08.016
– ident: e_1_3_4_68_2
  doi: 10.1038/jid.2009.429
– ident: e_1_3_4_4_2
  doi: 10.1189/jlb.0907654
– ident: e_1_3_4_25_2
  doi: 10.3389/fimmu.2019.00473
– ident: e_1_3_4_59_2
  doi: 10.1016/j.pain.2014.08.025
– ident: e_1_3_4_6_2
  doi: 10.1016/S0306-4522(02)00065-9
– ident: e_1_3_4_65_2
  doi: 10.1016/j.ejphar.2014.02.007
– ident: e_1_3_4_30_2
  doi: 10.1002/eji.200838659
– ident: e_1_3_4_73_2
  doi: 10.1517/17460441.2014.897324
– ident: e_1_3_4_14_2
  doi: 10.1016/j.immuni.2019.02.023
– ident: e_1_3_4_32_2
  doi: 10.1097/ALN.0b013e3182655f9f
– ident: e_1_3_4_44_2
  doi: 10.1186/1744-8069-4-63
– ident: e_1_3_4_45_2
  doi: 10.1016/S0304-3959(01)00433-X
– ident: e_1_3_4_13_2
  doi: 10.1073/pnas.1501372112
– ident: e_1_3_4_41_2
  doi: 10.1111/1346-8138.12440
– ident: e_1_3_4_51_2
  doi: 10.4049/jimmunol.164.8.4048
– ident: e_1_3_4_55_2
  doi: 10.1097/j.pain.0000000000001217
– ident: e_1_3_4_21_2
  doi: 10.1016/j.cyto.2018.11.030
– ident: e_1_3_4_23_2
  doi: 10.1016/j.jdermsci.2006.06.002
– ident: e_1_3_4_66_2
  doi: 10.1038/nri3683
– ident: e_1_3_4_27_2
  doi: 10.1002/eji.200324429
– ident: e_1_3_4_47_2
  doi: 10.1084/jem.187.1.129
– ident: e_1_3_4_48_2
  doi: 10.1084/jem.194.6.847
– ident: e_1_3_4_39_2
  doi: 10.1111/j.1399-3038.2011.01225.x
– ident: e_1_3_4_63_2
  doi: 10.1016/S0079-6123(00)29002-X
– ident: e_1_3_4_50_2
  doi: 10.4049/jimmunol.176.7.4331
– ident: e_1_3_4_8_2
  doi: 10.1111/j.1460-9568.2004.03514.x
SSID ssj0009580
Score 2.518816
Snippet Inflammatory pain, such as hypersensitivity resulting from surgical tissue injury, occurs as a result of interactions between the immune and nervous systems...
Interactions between the nervous and immune systems control the generation and maintenance of inflammatory pain. However, the immune cells and mediators...
SourceID pubmedcentral
proquest
crossref
pubmed
SourceType Open Access Repository
Aggregation Database
Index Database
StartPage 1
SubjectTerms Action Potentials
Animals
Biological Sciences
Biomarkers
CCL17 protein
CCL22 protein
Cell interactions
Chemokine CCL17 - genetics
Chemokine CCL17 - metabolism
Chemokine CCL22 - genetics
Chemokine CCL22 - metabolism
Chemokines
Dendritic cells
Dendritic structure
Disease Models, Animal
Disease Susceptibility
Excitability
Gene Expression Profiling
Hypersensitivity
Immune system
Inflammation
Injuries
Langerhans cells
Langerhans Cells - immunology
Langerhans Cells - metabolism
Mice
Myeloid cells
Neurons
Pain
Pain, Postoperative - diagnosis
Pain, Postoperative - etiology
Pain, Postoperative - metabolism
Pathogenesis
Receptors, CCR4 - metabolism
Sensory neurons
Sensory Receptor Cells - metabolism
Signal Transduction
siRNA
Skin
Tissues
Transgenic mice
Title Skin-resident dendritic cells mediate postoperative pain via CCR4 on sensory neurons
URI https://www.ncbi.nlm.nih.gov/pubmed/35046040
https://www.proquest.com/docview/2624686431
https://search.proquest.com/docview/2621659705
https://pubmed.ncbi.nlm.nih.gov/PMC8794894
Volume 119
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8QwEB50T17Et_VFBA966G6bJmn3KIuyCIqIgreSpCkuaLbYKvjvnaTt-rp56aFJH8xMMt8kM18ATlLO8b5GDaD7DJlMy1AqDFaY1BE3MhZCugLn6xsxfWBXj_xxCXhfC-OT9rWaDe3zy9DOnnxuZfWiR32e2Oj2epKhEWVjNlqGZXS_fYi-YNrN2roTitMvo6zn80mTUWVlPaQOUqOfih1haMLdxqBb-fjulf5Azd8Zk99c0OUarHbYkZy3_7gOS8ZuwHo3Omty2lFIn23CvTtTK8RI2h0Z2hC8FP5IA-LW6Wviy0UaQyrHqlGZlvybVHJmyftMksnkjpG5JTWGuPPXD-I5L229BQ-XF_eTadidnxBqRuMmTLOkUMJBgtLguMRwNOLacBrJMhmjm2ZjpgqlCsHKgmoEZpKVqTS00DQuRZYl2zCwc2t2gWiEMULpkhVcM5w_M1rEQpeRpgrfy0wAp7388qqlycj99naa5E7q-ZfUAzjo5Zt34wWbBWUiQ3QUB3C8aEZLd2KR1szffJ9YYPwT8QB2WnUsvtXrMYD0h6IWHRyL9s8WNC7Ppt0Z096_n9yHFepqIqI4pPwABs3rmzlEpNKoI-cn-JG3z0-jGemr
link.rule.ids 230,314,727,780,784,885,27924,27925,53791,53793
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB5RemgvFfSZAsWVONBDdmPHdrJHtAItj0WoWiRuke046krFG5FQiX_P2EkWaG-95BA7D82MPd_YM58BDjIh8L5BDaD7jLnKqlhpDFa4MomwikqpfIHz_FLOrvnZjbjZADHUwoSkfaOXI_f7duSWv0JuZX1rxkOe2PhqPs3RiPIJH7-C1yLNJnQI0tdcu3lXecJwAuaMD4w-WTqunWpGzINq9FTUU4amwm8N-rWP537pH7D5d87kMyd0sgXvevRIjrq_3IYN697Ddj8-G3LYk0j_-AALf6pWjLG0PzS0JXgpw6EGxK_UNyQUjLSW1J5Xo7Yd_Tep1dKRP0tFptOfnKwcaTDIXd09kMB66ZqPcH1yvJjO4v4EhdhwRts4y9NSSw8KKosjEwPSRBgrWKKqdIKOmk-4LrUuJa9KZhCaKV5lyrLSMFrJPE8_waZbOfsFiEEgI7WpeCkMxxk0ZyWVpkoM0_hebiM4HORX1B1RRhE2uLO08FIvnqQewe4g36IfMdgsGZc54iMawfd1M9q6F4tydnUf-lCJEVAiIvjcqWP9rUGPEWQvFLXu4Hm0X7ageQU-7d6cvv73k_vwZraYXxQXp5fnO_CW-QqJhMZM7MJme3dv9xC3tPpbsNJHlLzsGg
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BkRCXivIMlGIkDuWQTeLYTvaIlq7Ko1WFWqm3yE-xUuuNmhSJf8_YSZYt3LjkEDsPjceeb-yZbwDeV5zjfY0jgOYzZbJyqVTorDCpc25lIYQMCc4np-L4gn255Jdbpb5i0L5Wq5m_up751Y8YW9le62yKE8vOThY1KlE9Z1lrXHYfHvASlWxy1Dd8u_WQfUJxEWaUTaw-VZm1XnYzGoA1Wqsi0IaWPBwPhv2Pbdv0D-D8O25yyxAtH8PuiCDJx-FP9-Ce9U9gb5yjHTkciaQ_PIXzUFkrRX86FA7tCV5MLGxAwm59R2LSSG9JG7g1WjtQgJNWrjz5uZJksfjOyNqTDh3d9c0vEpkvffcMLpZH54vjdKyikGpGiz6t6tIoEYCBszg70SnNubac5tKVczTWbM6UUcoI5gzVCM8kc5W01GhaOFHX5XPY8WtvXwLRCGaE0o4ZrhmuojU1hdAu11The5lN4HCSX9MOZBlNPOSuyiZIvfkj9QT2J_k246zBZkGZqBEjFQm82zSjvgexSG_Xt7FPIdALynkCL4bh2HxrGscEqjsDtekQuLTvtqCKRU7tUaVe_feTb-Hh2adl8-3z6dfX8IiGJIm8SCnfh53-5ta-QejSq4OopL8BAw7tLQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Skin-resident+dendritic+cells+mediate+postoperative+pain+via+CCR4+on+sensory+neurons&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Raymondi+Silva%2C+Jaqueline&rft.au=Iftinca%2C+Mircea&rft.au=Fernandes+Gomes%2C+Francisco+I.&rft.au=Segal%2C+Julia+P.&rft.date=2022-01-25&rft.issn=0027-8424&rft.eissn=1091-6490&rft.volume=119&rft.issue=4&rft_id=info:doi/10.1073%2Fpnas.2118238119&rft.externalDBID=n%2Fa&rft.externalDocID=10_1073_pnas_2118238119
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0027-8424&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0027-8424&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0027-8424&client=summon