Retrieval of the mrp2 Gene Encoded Conjugate Export Pump from the Canalicular Membrane Contributes to Cholestasis Induced by tert-Butyl Hydroperoxide and Chloro-Dinitrobenzene

• Published in: Biological chemistry Vol. 381; no. 5-6; pp. 487 - 495
• Main Authors: Schmitt, M., Kubitz, R., Wettstein, M., Dahl, S. vom, Häussinger, D.
• Format: Journal Article
• Language: English
• Published: Germany Walter de Gruyter 01.05.2000
• Subjects: Animals; Bile Canaliculi - metabolism; Cholestasis - chemically induced; Cholestasis - genetics; Dinitrochlorobenzene - toxicity; Glutathione - metabolism; Liver - metabolism; Liver - ultrastructure; Male; Microscopy, Confocal; Mitochondrial Proteins; Rats; Rats, Wistar; Ribosomal Proteins - genetics; Saccharomyces cerevisiae Proteins; tert-Butylhydroperoxide - toxicity

Oxidative stress is known to induce cholestasis, but the underlying mechanisms are poorly understood. In this study we have characterized the short-term effects of tert-butyl hydroperoxide (t-BOOH)- and 1-chloro-2,4-dinitrobenzene (CDNB) on the mrp2 gene encoded canalicular export pump (Mrp2). The e...