Nitration of tryptophan in ribosomal proteins is a novel post-translational modification of differentiated and naïve PC12 cells

• Published in: Nitric oxide Vol. 25; no. 2; pp. 176 - 182
• Main Authors: Kawasaki, Hiroaki, Shigenaga, Ayako, Uda, Munehiro, Baba, Takeshi, Ogawa, Hideoki, Takamori, Kenji, Yamakura, Fumiyuki
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2011
• Subjects: 6-Nitrotryptophan; Animals; Blotting, Western; Cell Differentiation; Electrophoresis, Polyacrylamide Gel; Mass Spectrometry - methods; Nerve Growth Factor - pharmacology; NG-Nitroarginine Methyl Ester - pharmacology; NGF; Nitration; Nitric Oxide - metabolism; Nitric Oxide Synthase - antagonists & inhibitors; Nitric Oxide Synthase - metabolism; Nitric Oxide Synthase Type I; PC12 cell; PC12 Cells; Protein Processing, Post-Translational; Proteomics; Rats; Ribosomal protein; Ribosomal Proteins - metabolism; Tryptophan - analogs & derivatives; Tryptophan - metabolism; Nitration; L-NAME; RNS; nNOS; Proteomics; PC12 cell; LC–ESI–MS–MS; NGF; Ribosomal protein; RP; 6-Nitrotryptophan; D-MEM

Neuron growth factor (NGF) signaling in PC12 cell, which is derived from pheochromocytoma of rat adrenal medulla, induces expression of neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) production. Subsequently, NO causes differentiation of PC12 cell to neuronal cell with morphological cha...