Effect of Y-27632 on release of cytokines from peripheral T cells in asthmatic patients and normal subjects

• Published in: International immunopharmacology Vol. 4; no. 4; pp. 557 - 561
• Main Authors: Aihara, Masayuki, Dobashi, Kunio, Iizuka, Kunihiko, Nakazawa, Tsugio, Mori, Masatomo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2004; Elsevier
• Subjects: Adult; Amides - administration & dosage; Amides - pharmacology; Antineoplastic agents; Asthma - immunology; Asthma - metabolism; Biological and medical sciences; Bronchial asthma; Cells, Cultured; Concanavalin A; Concanavalin A - pharmacology; Cytokines - biosynthesis; Dose-Response Relationship, Drug; Female; Human T cells; Humans; IFN-gamma; IL-4; Interferon-gamma - metabolism; Interleukin-2 - metabolism; Interleukin-4 - metabolism; Interleukin-5 - metabolism; Intracellular Signaling Peptides and Proteins; Male; Medical sciences; Pharmacology. Drug treatments; Protein-Serine-Threonine Kinases - antagonists & inhibitors; Pyridines - administration & dosage; Pyridines - pharmacology; rho-Associated Kinases; T-Lymphocytes - drug effects; T-Lymphocytes - metabolism; Th1 Cells - metabolism; Th2 Cells - metabolism; Tumors; Y-27632; IL-4; Bronchial asthma; Y-27632; IFN-gamma; Concanavalin A; Human T cells; Human; Lung disease; Respiratory disease; Cytokine; Respiratory system; Asthma; Respiratory tract; Interleukin 4; T-Lymphocyte; Bronchus disease; Interferon; Obstructive pulmonary disease
• DOI: 10.1016/j.intimp.2003.12.014
• ISSN: 1567-5769

Previously we have reported that Y-27632, a selective inhibitor of Rho-associated protein kinases (ROCKs) for RhoA, suppressed concanavalin A (Con A)-induced secretion of cytokines from peripheral T cells of normal persons. Recent studies suggested its usefulness for clinical management of bronchial asthma. We examined effect of Y-27632 on release of cytokines from T cells of asthmatic patients. Peripheral T cells of six asthmatic subjects and six normal persons were stimulated with Con A for 24 h in the presence of Y-27632. The concentrations of IL-2, interferon (IFN)-gamma, IL-4, and IL-5 in supernatant were measured. Y-27632 down-regulated secretion of IL-2 and IFN-gamma and weakly decreased secretion of IL-4 and IL-5 from Con A-activated T cells in the asthmatics. The reduced secretion of IFN-gamma and IL-4 in the asthmatics was inferior to that in the normal subjects. Our data suggested that the involvement of RhoA/ROCK pathway in TCR-mediated secretion of IFN-gamma and IL-4 in patients with bronchial asthma differed from that in healthy persons.