Cloning and characterization of genomic DNA sequences of four self-incompatibility alleles in sweet cherry (Prunus avium L.)

• Published in: Theoretical and applied genetics Vol. 108; no. 2; pp. 299 - 305
• Main Authors: WÜNSCH, A, HORMAZA, J. I
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 2004; Berlin Springer Nature B.V
• Subjects: 5' Untranslated Regions - genetics; Alleles; Allelomorphism; Amino Acid Sequence; Amino acids; Analysis; Base Sequence; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Cloning; Cloning, Molecular; Cultivars; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Complementary; DNA, Plant - genetics; Ethylenediaminetetraacetic acid; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetic research; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Molecular Sequence Data; Nucleotide sequencing; Prunus - enzymology; Prunus - genetics; Prunus avium; Pteridophyta, spermatophyta; Ribonuclease; Ribonucleases - chemistry; Ribonucleases - genetics; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Species Specificity; Vegetals; Japan; Spain; Incompatibility; Cherry; Enzyme; Genomics; Rosaceae; Esterases; Self-incompatibility; Characterization; Allele; S-allele; Dicotyledones; DNA; Prunus avium; Angiospermae; Prunus; Hydrolases; Genetics; Spermatophyta; Agriculture; Pancreatic ribonuclease; RNase

Gametophytic self-incompatibility (GSI) in sweet cherry is determined by a locus S with multiple alleles. In the style, the S-locus codifies for an allele-specific ribonuclease ( S-RNase) that is involved in the rejection of pollen that carries the same S allele. In this work we report the cloning a...