Conformation and dynamic interactions of the multipartite genome in Agrobacterium tumefaciens

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 6; p. 1
• Main Authors: Ren, Zhongqing, Liao, Qin, Karaboja, Xheni, Barton, Ian S, Schantz, Eli G, Mejia-Santana, Adrian, Fuqua, Clay, Wang, Xindan
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 08.02.2022
• Subjects: Agrobacterium tumefaciens; Agrobacterium tumefaciens - genetics; Bacterial Proteins - genetics; Biological Sciences; Cell cycle; Cell Cycle - genetics; Chromosomes; Chromosomes, Bacterial; Clustering; Conformation; Fluorescence; Fluorescence microscopy; Genome, Bacterial; Genomes; Maintenance; Origins; Plasmids; Replication origins; Replicon; Agrobacterium tumefaciens; ChIP-seq; SMC; ParB; Hi-C

Bacterial species from diverse phyla contain multiple replicons, yet how these multipartite genomes are organized and segregated during the cell cycle remains poorly understood. has a 2.8-Mb circular chromosome (Ch1), a 2.1-Mb linear chromosome (Ch2), and two large plasmids (pAt and pTi). We used th...