Gene Array-Based Identification of Changes That Contribute to Ethanol Tolerance in Ethanologenic Escherichia coli: Comparison of KO11 (Parent) to LY01 (Resistant Mutant)

• Published in: Biotechnology progress Vol. 19; no. 2; pp. 612 - 623
• Main Authors: Gonzalez, Ramon, Tao, Han, Purvis, J. E., York, S. W., Shanmugam, K. T., Ingram, L. O.
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 01.03.2003; American Institute of Chemical Engineers
• Subjects: Biological and medical sciences; Directed Molecular Evolution - methods; Drug Resistance, Microbial - genetics; Escherichia coli - classification; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - metabolism; Ethanol - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Gene Expression Regulation, Bacterial - physiology; Genetic Enhancement - methods; Oligonucleotide Array Sequence Analysis - methods

Escherichia coli KO11 (parent) and LY01 (mutant) have been engineered for the production of ethanol. Gene arrays were used to identify expression changes that occurred in the mutant, LY01, during directed evolution to improve ethanol tolerance (defined as extent of growth in the presence of added et...