Color-related chlorophyll and carotenoid concentrations of Chinese kale can be altered through CRISPR/Cas9 targeted editing of the carotenoid isomerase gene BoaCRTISO

• Published in: Horticulture research Vol. 7; no. 1; p. 161
• Main Authors: Sun, Bo, Jiang, Min, Zheng, Hao, Jian, Yue, Huang, Wen-Li, Yuan, Qiao, Zheng, Ai-Hong, Chen, Qing, Zhang, Yun-Ting, Lin, Yuan-Xiu, Wang, Yan, Wang, Xiao-Rong, Wang, Qiao-Mei, Zhang, Fen, Tang, Hao-Ru
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 2020; Oxford University Press
• Subjects: 631/449/2491/2046; 631/449/2667; 631/449/447/2311; Agriculture; Biomedical and Life Sciences; Biosynthesis; Brassica; Brassica alboglabra; Carotenoids; Chlorophyll; Color; CRISPR; Ecology; Life Sciences; Mutants; Mutation; Mutation rates; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; Quality control; Vegetables; Molecular engineering in plants; Secondary metabolism; Transgenic plants

The carotenoid isomerase gene ( BoaCRTISO ) of Chinese kale was targeted and edited using the CRISPR/Cas9 system in the present study. The results showed a high mutation rate (81.25%), and 13 crtiso mutants were obtained. Only two types of mutations, insertions and replacements, were found. Both the...