Report on stage III Pig-a mutation assays using N-ethyl-N-nitrosourea - comparison with other in vivo genotoxicity endpoints

• Published in: Environmental and molecular mutagenesis Vol. 52; no. 9; pp. 721 - 730
• Main Authors: Cammerer, Zoryana, Bhalli, Javed A., Cao, Xuefei, Coffing, Stephanie L., Dickinson, Donna, Dobo, Krista L., Dobrovolsky, Vasily N., Engel, Maria, Fiedler, Ronald D., Gunther, William C., Heflich, Robert H., Pearce, Mason G., Shaddock, Joseph G., Shutsky, Thomas, Thiffeault, Catherine J., Schuler, Maik
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.12.2011; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Calibration; CD59; CD59 Antigens - genetics; Colon - drug effects; Colon - ultrastructure; comet assay; Comet Assay - methods; Comet Assay - standards; Data Interpretation, Statistical; Diethylnitrosamine - toxicity; Dose-Response Relationship, Drug; Endpoint Determination; Erythrocytes - drug effects; Erythrocytes - ultrastructure; Flow Cytometry; Fundamental and applied biological sciences. Psychology; gene mutation; Genetics of eukaryotes. Biological and molecular evolution; Hprt; Liver - drug effects; Liver - ultrastructure; Male; Medical sciences; Membrane Proteins - genetics; micronucleus assay; Micronucleus Tests - methods; Micronucleus Tests - standards; Mutagenicity Tests - methods; Mutagenicity Tests - standards; Mutagens - toxicity; Mutation; Organ Specificity; Rats; Rats, Inbred F344; Rats, Sprague-Dawley; Reference Standards; Reproducibility of Results; reticulocytes; Reticulocytes - drug effects; Reticulocytes - ultrastructure; Species Specificity; Spleen - drug effects; Spleen - ultrastructure; Time Factors; Toxicology; Flow cytometry; Rat; Toxicity; Nitrosoureas; Glycosyltransferases; Genotoxicity; Hprt; Hypoxanthine phosphoribosyltransferase; Comet assay; micronucleus assay; Gene; Micronucleus; CD59; rats; Ungulata; Enzyme; Report; Mutagenicity testing; Transferases; gene mutation; Rodentia; reticulocytes; Pig; In vivo; Vertebrata; Mammalia; Reticulocyte; Artiodactyla; Mutation; Pentosyltransferases

N‐Ethyl‐N‐nitrosourea (ENU) was evaluated as part of the Stage III trial for the rat Pig‐a gene mutation assay. Groups of six‐ to eight‐week‐old male Sprague Dawley (SD) or Fischer 344 (F344) rats were given 28 daily doses of the phosphate buffered saline vehicle, or 2.5, 5, or 10 mg/kg ENU, and eva...