An implication of novel methodology to study pancreatic acinar mitochondria under in situ conditions
Mitochondria maintain numerous energy‐consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreati...
Saved in:
| Published in | Cell biochemistry and function Vol. 31; no. 2; pp. 115 - 121 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Blackwell Publishing Ltd
01.03.2013
Wiley Subscription Services, Inc |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Mitochondria maintain numerous energy‐consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ. Here we propose a novel experimental protocol suitable for in situ analysis of pancreatic mitochondria metabolic states. Isolated rat pancreatic acini were permeabilized with low doses of digitonin. Different metabolic states of mitochondria were examined in KCl‐ and sucrose‐based solutions using Clark oxygen electrode. Respiration of digitonin‐treated, unlike of intact, acini was substantially intensified by succinate or mixture of pyruvate plus malate. Substrate‐stimulated respiration rate did not depend on solution composition. In sucrose‐based solution, oligomycin inhibited State 3 respiration at succinate oxidation by 65.4% and at pyruvate plus malate oxidation by 60.2%, whereas in KCl‐based solution, by 32.0% and 36.1%, respectively. Apparent respiratory control indices were considerably higher in sucrose‐based solution. Rotenone or thenoyltrifluoroacetone severely inhibited respiration, stimulated by pyruvate plus malate or succinate, respectively. This revealed low levels of non‐mitochondrial oxygen consumption of permeabilized acinar cells. These results suggest a stronger coupling between respiration and oxidative phosphorylation in sucrose‐based solution. Copyright © 2012 John Wiley & Sons, Ltd. |
|---|---|
| AbstractList | Mitochondria maintain numerous energy-consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ. Here we propose a novel experimental protocol suitable for in situ analysis of pancreatic mitochondria metabolic states. Isolated rat pancreatic acini were permeabilized with low doses of digitonin. Different metabolic states of mitochondria were examined in KCl- and sucrose-based solutions using Clark oxygen electrode. Respiration of digitonin-treated, unlike of intact, acini was substantially intensified by succinate or mixture of pyruvate plus malate. Substrate-stimulated respiration rate did not depend on solution composition. In sucrose-based solution, oligomycin inhibited State 3 respiration at succinate oxidation by 65.4% and at pyruvate plus malate oxidation by 60.2%, whereas in KCl-based solution, by 32.0% and 36.1%, respectively. Apparent respiratory control indices were considerably higher in sucrose-based solution. Rotenone or thenoyltrifluoroacetone severely inhibited respiration, stimulated by pyruvate plus malate or succinate, respectively. This revealed low levels of non-mitochondrial oxygen consumption of permeabilized acinar cells. These results suggest a stronger coupling between respiration and oxidative phosphorylation in sucrose-based solution. Mitochondria maintain numerous energy‐consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ. Here we propose a novel experimental protocol suitable for in situ analysis of pancreatic mitochondria metabolic states. Isolated rat pancreatic acini were permeabilized with low doses of digitonin. Different metabolic states of mitochondria were examined in KCl‐ and sucrose‐based solutions using Clark oxygen electrode. Respiration of digitonin‐treated, unlike of intact, acini was substantially intensified by succinate or mixture of pyruvate plus malate. Substrate‐stimulated respiration rate did not depend on solution composition. In sucrose‐based solution, oligomycin inhibited State 3 respiration at succinate oxidation by 65.4% and at pyruvate plus malate oxidation by 60.2%, whereas in KCl‐based solution, by 32.0% and 36.1%, respectively. Apparent respiratory control indices were considerably higher in sucrose‐based solution. Rotenone or thenoyltrifluoroacetone severely inhibited respiration, stimulated by pyruvate plus malate or succinate, respectively. This revealed low levels of non‐mitochondrial oxygen consumption of permeabilized acinar cells. These results suggest a stronger coupling between respiration and oxidative phosphorylation in sucrose‐based solution. Copyright © 2012 John Wiley & Sons, Ltd. Mitochondria maintain numerous energy-consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ. Here we propose a novel experimental protocol suitable for in situ analysis of pancreatic mitochondria metabolic states. Isolated rat pancreatic acini were permeabilized with low doses of digitonin. Different metabolic states of mitochondria were examined in KCl- and sucrose-based solutions using Clark oxygen electrode. Respiration of digitonin-treated, unlike of intact, acini was substantially intensified by succinate or mixture of pyruvate plus malate. Substrate-stimulated respiration rate did not depend on solution composition. In sucrose-based solution, oligomycin inhibited State 3 respiration at succinate oxidation by 65.4% and at pyruvate plus malate oxidation by 60.2%, whereas in KCl-based solution, by 32.0% and 36.1%, respectively. Apparent respiratory control indices were considerably higher in sucrose-based solution. Rotenone or thenoyltrifluoroacetone severely inhibited respiration, stimulated by pyruvate plus malate or succinate, respectively. This revealed low levels of non-mitochondrial oxygen consumption of permeabilized acinar cells. These results suggest a stronger coupling between respiration and oxidative phosphorylation in sucrose-based solution. Copyright © 2012 John Wiley & Sons, Ltd. [PUBLICATION ABSTRACT] Mitochondria maintain numerous energy‐consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ . Here we propose a novel experimental protocol suitable for in situ analysis of pancreatic mitochondria metabolic states. Isolated rat pancreatic acini were permeabilized with low doses of digitonin. Different metabolic states of mitochondria were examined in KCl‐ and sucrose‐based solutions using Clark oxygen electrode. Respiration of digitonin‐treated, unlike of intact, acini was substantially intensified by succinate or mixture of pyruvate plus malate. Substrate‐stimulated respiration rate did not depend on solution composition. In sucrose‐based solution, oligomycin inhibited State 3 respiration at succinate oxidation by 65.4% and at pyruvate plus malate oxidation by 60.2%, whereas in KCl‐based solution, by 32.0% and 36.1%, respectively. Apparent respiratory control indices were considerably higher in sucrose‐based solution. Rotenone or thenoyltrifluoroacetone severely inhibited respiration, stimulated by pyruvate plus malate or succinate, respectively. This revealed low levels of non‐mitochondrial oxygen consumption of permeabilized acinar cells. These results suggest a stronger coupling between respiration and oxidative phosphorylation in sucrose‐based solution. Copyright © 2012 John Wiley & Sons, Ltd. |
| Author | Manko, Volodymyr V. Klevets, Myron Yu Manko, Bohdan O. |
| Author_xml | – sequence: 1 givenname: Bohdan O. surname: Manko fullname: Manko, Bohdan O. email: mankobo@gmail.com organization: Department of Human and Animal Physiology, Ivan Franko National University of Lviv, Lviv, Ukraine – sequence: 2 givenname: Myron Yu surname: Klevets fullname: Klevets, Myron Yu organization: Department of Human and Animal Physiology, Ivan Franko National University of Lviv, Lviv, Ukraine – sequence: 3 givenname: Volodymyr V. surname: Manko fullname: Manko, Volodymyr V. organization: Department of Human and Animal Physiology, Ivan Franko National University of Lviv, Lviv, Ukraine |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/22886484$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1kMFO3DAQQC1EVRZaiS9AlnrhErA9ju0c6dKFSrS9tOrRcmwHDIm9tZO2-_fNimUPSD2NNHp6M3rH6DCm6BE6peSCEsIubdtdMCX4AVpQ0jQVUZwfogVhAirBFT9Cx6U8EkIaAeQtOmJMqe1-gdxVxGFY98GaMaSIU4dj-u17PPjxIbnUp_sNHhMu4-Q2eG2izX4mLTY2RJPxEMZkH1J0ORg8ReczDhGXME7YztuwlZZ36E1n-uLf7-YJ-rH69H15W919u_m8vLqrLGeUV7R2nQFHJSMOCLOsa2oGIKUUABScc7Jl3EDtOSEgnGkNq2vZtJy3oJiFE3T-7F3n9GvyZdRDKNb3vYk-TUVToFw1DJSa0Q-v0Mc05Th_pykXNRMNn0_uhTanUrLv9DqHweSNpkRvy-u5vN6Wn9GznXBqB-_24EvqGaiegT-h95v_ivTy42on3PGhjP7vnjf5SQsJstY_v95oQb4QuL6VegX_AKc3nHA |
| CitedBy_id | crossref_primary_10_15407_ubj95_05_051 crossref_primary_10_15407_fz59_05_061 crossref_primary_10_1111_apha_12119 crossref_primary_10_15407_ubj91_03_034 crossref_primary_10_15407_ubj85_04_048 crossref_primary_10_1097_MPA_0000000000001864 crossref_primary_10_30970_sbi_1703_735 crossref_primary_10_1002_elsc_201400175 |
| Cites_doi | 10.1016/0005-2736(71)90123-4 10.1016/0005-2736(83)90001-9 10.1152/ajpendo.1978.235.5.E517 10.1073/pnas.77.6.3430 10.1042/CBI20110017 10.1016/S1567-7249(01)00025-3 10.1053/j.gastro.2010.01.037 10.1016/j.bbamem.2010.01.005 10.1016/S0021-9258(19)57191-5 10.1042/BJ20110162 10.1093/oxfordjournals.jbchem.a134941 10.1016/0006-291X(84)90216-X 10.1136/gut.2007.147207 10.1097/00024382-199503000-00005 10.2337/diabetes.50.2.361 10.1074/jbc.M412694200 10.1113/jphysiol.1981.sp013995 10.1042/bj1920951 10.1038/nprot.2008.61 10.1161/01.CIR.0000058462.23347.93 10.1007/s004240050167 10.1016/j.febslet.2010.01.035 10.2527/2001.7951329x 10.1085/jgp.106.6.1225 10.1016/S0021-9258(19)85914-8 10.1023/A:1006988625831 10.1177/153537020122600606 10.1042/bj1880207 10.1152/physrev.00004.2005 10.1016/0304-4165(91)90168-G 10.1074/jbc.M107794200 10.1042/bj1070807 10.1073/pnas.0904818106 10.4319/lo.1966.11.2.0264 |
| ContentType | Journal Article |
| Copyright | Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd. |
| Copyright_xml | – notice: Copyright © 2012 John Wiley & Sons, Ltd. – notice: Copyright © 2013 John Wiley & Sons, Ltd. |
| DBID | BSCLL CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QP 7QR 7TK 7TM 7U7 8FD C1K FR3 P64 RC3 7X8 |
| DOI | 10.1002/cbf.2864 |
| DatabaseName | Istex Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Toxicology Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Genetics Abstracts Technology Research Database Toxicology Abstracts Nucleic Acids Abstracts Chemoreception Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Genetics Abstracts MEDLINE CrossRef |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry Biology |
| EISSN | 1099-0844 |
| EndPage | 121 |
| ExternalDocumentID | 3147488281 10_1002_cbf_2864 22886484 CBF2864 ark_67375_WNG_60M03DH7_F |
| Genre | article Journal Article |
| GroupedDBID | --- .3N .GA .Y3 05W 0R~ 10A 1L6 1OB 1OC 1ZS 29B 31~ 33P 3SF 3WU 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52R 52S 52T 52U 52V 52W 52X 53G 5GY 5VS 66C 6J9 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A01 A03 AAESR AAEVG AAHHS AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABEML ABIJN ABPVW ABQWH ABXGK ACAHQ ACBWZ ACCFJ ACCZN ACGFO ACGFS ACGOF ACIWK ACMXC ACPOU ACPRK ACSCC ACXBN ACXQS ADBBV ADBTR ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFPM AFGKR AFPWT AFRAH AFZJQ AHBTC AIACR AIAGR AITYG AIURR AIWBW AJBDE ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AVWKF AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BLYAC BMXJE BROTX BRXPI BSCLL BY8 C45 CS3 D-6 D-7 D-E D-F DCZOG DPXWK DR1 DR2 DRFUL DRMAN DRSTM DU5 EBD EBS EJD EMOBN F00 F01 F04 F5P FEDTE FUBAC G-S G.N GNP GODZA H.X HF~ HGLYW HHY HHZ HVGLF HZ~ IX1 J0M JPC KBYEO KQQ LATKE LAW LC2 LC3 LEEKS LH4 LH6 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM N04 N05 N9A NDZJH NF~ O66 O9- OIG OVD P2P P2W P2X P2Z P4B P4D PALCI Q.N Q11 QB0 QRW R.K RBB RIWAO RJQFR ROL RWI RX1 RYL SAMSI SUPJJ SV3 TEORI UB1 V2E V8K W8V W99 WBKPD WH7 WIB WIH WIJ WIK WJL WNSPC WOHZO WQJ WRC WSB WXI WXSBR WYISQ XG1 XPP XV2 ZZTAW ~IA ~WT CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QP 7QR 7TK 7TM 7U7 8FD C1K FR3 P64 RC3 7X8 |
| ID | FETCH-LOGICAL-c4214-15dfa3d1720d302c2f9523377763313ddd7b24a35e40036daba25579b44b382c3 |
| IEDL.DBID | DR2 |
| ISSN | 0263-6484 |
| IngestDate | Sat Aug 17 00:24:18 EDT 2024 Thu Oct 10 15:37:42 EDT 2024 Fri Aug 23 01:49:20 EDT 2024 Sat Sep 28 07:51:52 EDT 2024 Sat Aug 24 01:11:01 EDT 2024 Wed Oct 30 09:49:12 EDT 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Language | English |
| License | Copyright © 2012 John Wiley & Sons, Ltd. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4214-15dfa3d1720d302c2f9523377763313ddd7b24a35e40036daba25579b44b382c3 |
| Notes | istex:7E41F1AB5FE19C61117966363D21421B2BC12C25 ArticleID:CBF2864 ark:/67375/WNG-60M03DH7-F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cbf.2864 |
| PMID | 22886484 |
| PQID | 1465269431 |
| PQPubID | 2029981 |
| PageCount | 7 |
| ParticipantIDs | proquest_miscellaneous_1314892388 proquest_journals_1465269431 crossref_primary_10_1002_cbf_2864 pubmed_primary_22886484 wiley_primary_10_1002_cbf_2864_CBF2864 istex_primary_ark_67375_WNG_60M03DH7_F |
| PublicationCentury | 2000 |
| PublicationDate | 2013-03 March 2013 2013-Mar 2013-03-00 20130301 |
| PublicationDateYYYYMMDD | 2013-03-01 |
| PublicationDate_xml | – month: 03 year: 2013 text: 2013-03 |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: Bognor Regis |
| PublicationTitle | Cell biochemistry and function |
| PublicationTitleAlternate | Cell Biochem Funct |
| PublicationYear | 2013 |
| Publisher | Blackwell Publishing Ltd Wiley Subscription Services, Inc |
| Publisher_xml | – name: Blackwell Publishing Ltd – name: Wiley Subscription Services, Inc |
| References | Villalobo A, Lehninger AL. Inhibition of oxidative phosphorylation in ascites tumor mitochondria and cells by intramitochondrial Ca2+. J Biol Chem 1980; 255: 2457-2464. Streb H, Schulz I. Regulation of cytosolic free Ca2+ concentration in acinar cells of rat pancreas. Am J Physiol 1983; 245: G347-G357. Williams JA, Korc M, Dormer RL. Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am J Physiol 1978; 235: 517-524. Voronina SG, Barrow SL, Simpson AW, et al. Dynamic changes in cytosolic and mitochondrial ATP levels in pancreatic acinar cells. Gastroenterology 2010; 138: 1976-1987. Krause E, Gobel A, Schulz I. Cell side-specific sensitivities of intracellular Ca2+ stores for inositol 1,4,5-trisphosphate, cyclic ADP-ribose, and nicotinic acid adenine dinucleotide phosphate in permeabilized pancreatic acinar cells from mouse. J Biol Chem 2002; 277: 11696-11702. Sasaki S, Nakagaki I, Kondo H, Hori S. Changes in element concentrations induced by agonist in pig pancreatic acinar cells. Pflugers Arch 1996; 432: 538-545. Soboll S, Akerboom TP, Schwenke WD, Haase R, Sies H. Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non-aqueous fractionation procedure. Biochem J 1980; 192: 951-954. Kondrashova MN, Fedotcheva NI, Saakyan IR, et al. Preservation of native properties of mitochondria in rat liver homogenate. Mitochondrion 2001; 1: 249-267. Brocks DG, Siess EA, Wieland OH. Validity of the digitonin method for metabolite compartmentation in isolated hepatocytes. Biochem J 1980; 188: 207-212. Colbeau A, Nachbaur J, Vignais PM. Enzymic characterization and lipid composition of rat liver subcellular membranes. Biochim Biophys Acta 1971; 249: 462-492. Saks VA, Belikova YO, Kuznetsov AV. In vivo regulation of mitochondrial respiration in cardiomyocytes: specific restrictions for intracellular diffusion of ADP. Biochim Biophys Acta 1991; 1074: 302-311. Babsky A, Doliba NIC, Doliba NAT, Savchenko A, Wehrli S, Osbakken M. Na+ effects on mitochondrial respiration and oxidative phosphorylation in diabetic hearts. Exp Biol Med 2001; 226: 543-551. Nishikawa M, Nojima S, Akiyama T, Sankawa U, Inoue K. Interaction of digitonin and its analogs with membrane cholesterol. J Biochem 1984; 96: 1231-1239. [ Shostakovska IV, Doliba MM. Cholinergic regulation of energy exchange in mitochondria of secretory organs. Visnyk of Lviv university 1989; 19: 3-19] - in Russian. Akar JG, Everett TH, Ho R, et al. Intracellular chloride accumulation and subcellular elemental distribution during atrial fibrillation. Circulation 2003; 107: 1810-1815. Wilson JS, Korsten MA, Lieber CS. The isolation and properties of mitochondria from rat pancreas. Biochem Biophys Res Commun 1984; 121: 545-551. Rizzuto R, Pozzan T. Microdomains of intracellular Ca2+: molecular determinants and functional consequences. Physiol Rev 2006; 86: 369-408. Brand MD, Nicholls DG. Assessing mitochondrial dysfunction in cells. Biochem J 2011; 435: 297-312. Carpenter JH. New measurements of oxygen solubility in pure and natural water. Limnol Oceanogr 1966; 11: 264-277. Lucas M, Galvan A, Solano P, Goberna R. Compartmentation of calcium in digitonin-disrupted guinea pig pancreatic acinar cells. Biochim Biophys Acta 1983; 731: 129-136. Schulz HU, Pross M, Meyer F, Matthias R, Halangk W. Acinar cell respiration in experimental acute pancreatitis. Shock 1995; 3: 184-188. Tomaskova Z, Ondrias K. Mitochondrial chloride channels--What are they for? FEBS Lett 2010; 584: 2085-2092. Preissler M, Williams JA. Pancreatic acinar cell function: measurement of intracellular ions and pH and their relation to secretion. J Physiol 1981; 321: 437-448. Schild L, Matthias R, Stanarius A, Wolf G, Augustin W, Halangk W. Induction of permeability transition in pancreatic mitochondria by cerulein in rats. Mol Cell Biochem 1999; 195: 191-197. Howell JA, Matthews AD, Swanson KC, Harmon DL, Matthews JC. Molecular identification of high-affinity glutamate transporters in sheep and cattle forestomach, intestine, liver, kidney, and pancreas. J Anim Sci 2001; 79: 1329-1336. Horbay RO, Manko BO, Manko VV, Lootsik MD, Stoika RS. Respiration characteristics of mitochondria in parental and giant transformed cells of the murine Nemeth-Kellner lymphoma. Cell Biol Int 2012; 36: 71-77. Chance B, Williams GR. Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 1955; 217: 409-427. Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS. Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat Protoc 2008; 3: 965-976. Dolman NJ, Gerasimenko JV, Gerasimenko OV, Voronina SG, Petersen OH, Tepikin AV. Stable Golgi-mitochondria complexes and formation of Golgi Ca(2+) gradients in pancreatic acinar cells. J Biol Chem 2005; 280: 15794-15799. Zhao H, Muallem S. Na+, K+, and Cl- transport in resting pancreatic acinar cells. J Gen Physiol 1995; 106: 1225-1242. Zhao C, Wilson MC, Schuit F, Halestrap AP, Rutter GA. Expression and distribution of lactate/monocarboxylate transporter isoforms in pancreatic islets and the exocrine pancreas. Diabetes 2001; 50: 361-366. Corcelli A, Saponetti MS, Zaccagnino P, et al. Mitochondria isolated in nearly isotonic KCl buffer: focus on cardiolipin and organelle morphology. Biochim Biophys Acta 2010; 1798: 681-687. Fiskum G, Craig SW, Decker GL, Lehninger AL. The cytoskeleton of digitonin-treated rat hepatocytes. Proc Natl Acad Sci USA 1980; 77: 3430-3434. Odinokova IV, Sung KF, Mareninova OA, et al. Mechanisms regulating cytochrome c release in pancreatic mitochondria. Gut 2009; 58: 431-442. Gerasimenko JV, Lur G, Sherwood MW, et al. Pancreatic protease activation by alcohol metabolite depends on Ca2+ release via acid store IP3 receptors. Proc Natl Acad Sci USA 2009; 106: 10758-10763. Hems R, Stubbs M, Krebs HA. Restricted permeability of rat liver for glutamate and succinate. Biochem J 1968; 107: 807-815. 2011; 435 1983; 731 2001; 50 1968; 107 1984; 121 1991; 1074 1981; 321 1955; 217 2002; 277 1971; 249 2010; 584 1980; 192 2008; 3 1978; 235 2012; 36 1995; 3 1966; 11 1980; 255 2010; 1798 2001; 226 2009; 58 2005; 280 1984; 96 1983; 245 2006; 86 2003; 107 2010; 138 1980; 77 1995; 106 1999; 195 2001; 1 1996; 432 2001; 79 1989; 19 1980; 188 2009; 106 e_1_2_7_6_1 e_1_2_7_5_1 e_1_2_7_4_1 e_1_2_7_3_1 e_1_2_7_9_1 e_1_2_7_7_1 e_1_2_7_19_1 e_1_2_7_18_1 e_1_2_7_17_1 e_1_2_7_2_1 e_1_2_7_15_1 Streb H (e_1_2_7_16_1) 1983; 245 e_1_2_7_14_1 e_1_2_7_13_1 e_1_2_7_12_1 e_1_2_7_11_1 e_1_2_7_26_1 e_1_2_7_27_1 e_1_2_7_28_1 e_1_2_7_29_1 e_1_2_7_30_1 e_1_2_7_25_1 e_1_2_7_31_1 e_1_2_7_24_1 e_1_2_7_32_1 e_1_2_7_23_1 e_1_2_7_33_1 e_1_2_7_22_1 e_1_2_7_34_1 e_1_2_7_21_1 e_1_2_7_35_1 e_1_2_7_20_1 e_1_2_7_36_1 e_1_2_7_37_1 Shostakovska IV (e_1_2_7_8_1) 1989; 19 Villalobo A (e_1_2_7_10_1) 1980; 255 |
| References_xml | – volume: 255 start-page: 2457 year: 1980 end-page: 2464 article-title: Inhibition of oxidative phosphorylation in ascites tumor mitochondria and cells by intramitochondrial Ca publication-title: J Biol Chem – volume: 1798 start-page: 681 year: 2010 end-page: 687 article-title: Mitochondria isolated in nearly isotonic KCl buffer: focus on cardiolipin and organelle morphology publication-title: Biochim Biophys Acta – volume: 11 start-page: 264 year: 1966 end-page: 277 article-title: New measurements of oxygen solubility in pure and natural water publication-title: Limnol Oceanogr – volume: 19 start-page: 3 year: 1989 end-page: 19 article-title: Cholinergic regulation of energy exchange in mitochondria of secretory organs publication-title: Visnyk of Lviv university – volume: 50 start-page: 361 year: 2001 end-page: 366 article-title: Expression and distribution of lactate/monocarboxylate transporter isoforms in pancreatic islets and the exocrine pancreas publication-title: Diabetes – volume: 195 start-page: 191 year: 1999 end-page: 197 article-title: Induction of permeability transition in pancreatic mitochondria by cerulein in rats publication-title: Mol Cell Biochem – volume: 3 start-page: 965 year: 2008 end-page: 976 article-title: Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells publication-title: Nat Protoc – volume: 245 start-page: G347 year: 1983 end-page: G357 article-title: Regulation of cytosolic free Ca concentration in acinar cells of rat pancreas publication-title: Am J Physiol – volume: 86 start-page: 369 year: 2006 end-page: 408 article-title: Microdomains of intracellular Ca : molecular determinants and functional consequences publication-title: Physiol Rev – volume: 321 start-page: 437 year: 1981 end-page: 448 article-title: Pancreatic acinar cell function: measurement of intracellular ions and pH and their relation to secretion publication-title: J Physiol – volume: 77 start-page: 3430 year: 1980 end-page: 3434 article-title: The cytoskeleton of digitonin‐treated rat hepatocytes publication-title: Proc Natl Acad Sci USA – volume: 107 start-page: 1810 year: 2003 end-page: 1815 article-title: Intracellular chloride accumulation and subcellular elemental distribution during atrial fibrillation publication-title: Circulation – volume: 435 start-page: 297 year: 2011 end-page: 312 article-title: Assessing mitochondrial dysfunction in cells publication-title: Biochem J – volume: 235 start-page: 517 year: 1978 end-page: 524 article-title: Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini publication-title: Am J Physiol – volume: 96 start-page: 1231 year: 1984 end-page: 1239 article-title: Interaction of digitonin and its analogs with membrane cholesterol publication-title: J Biochem – volume: 188 start-page: 207 year: 1980 end-page: 212 article-title: Validity of the digitonin method for metabolite compartmentation in isolated hepatocytes publication-title: Biochem J – volume: 280 start-page: 15794 year: 2005 end-page: 15799 article-title: Stable Golgi‐mitochondria complexes and formation of Golgi Ca( ) gradients in pancreatic acinar cells publication-title: J Biol Chem – volume: 584 start-page: 2085 year: 2010 end-page: 2092 article-title: Mitochondrial chloride channels‐‐What are they for? publication-title: FEBS Lett – volume: 58 start-page: 431 year: 2009 end-page: 442 article-title: Mechanisms regulating cytochrome c release in pancreatic mitochondria publication-title: Gut – volume: 277 start-page: 11696 year: 2002 end-page: 11702 article-title: Cell side‐specific sensitivities of intracellular Ca stores for inositol 1,4,5‐trisphosphate, cyclic ADP‐ribose, and nicotinic acid adenine dinucleotide phosphate in permeabilized pancreatic acinar cells from mouse publication-title: J Biol Chem – volume: 1074 start-page: 302 year: 1991 end-page: 311 article-title: In vivo regulation of mitochondrial respiration in cardiomyocytes: specific restrictions for intracellular diffusion of ADP publication-title: Biochim Biophys Acta – volume: 192 start-page: 951 year: 1980 end-page: 954 article-title: Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non‐aqueous fractionation procedure publication-title: Biochem J – volume: 731 start-page: 129 year: 1983 end-page: 136 article-title: Compartmentation of calcium in digitonin‐disrupted guinea pig pancreatic acinar cells publication-title: Biochim Biophys Acta – volume: 226 start-page: 543 year: 2001 end-page: 551 article-title: Na effects on mitochondrial respiration and oxidative phosphorylation in diabetic hearts publication-title: Exp Biol Med – volume: 249 start-page: 462 year: 1971 end-page: 492 article-title: Enzymic characterization and lipid composition of rat liver subcellular membranes publication-title: Biochim Biophys Acta – volume: 79 start-page: 1329 year: 2001 end-page: 1336 article-title: Molecular identification of high‐affinity glutamate transporters in sheep and cattle forestomach, intestine, liver, kidney, and pancreas publication-title: J Anim Sci – volume: 106 start-page: 10758 year: 2009 end-page: 10763 article-title: Pancreatic protease activation by alcohol metabolite depends on Ca release via acid store IP receptors publication-title: Proc Natl Acad Sci USA – volume: 107 start-page: 807 year: 1968 end-page: 815 article-title: Restricted permeability of rat liver for glutamate and succinate publication-title: Biochem J – volume: 36 start-page: 71 year: 2012 end-page: 77 article-title: Respiration characteristics of mitochondria in parental and giant transformed cells of the murine Nemeth‐Kellner lymphoma publication-title: Cell Biol Int – volume: 217 start-page: 409 year: 1955 end-page: 427 article-title: Respiratory enzymes in oxidative phosphorylation. III. The steady state publication-title: J Biol Chem – volume: 138 start-page: 1976 year: 2010 end-page: 1987 article-title: Dynamic changes in cytosolic and mitochondrial ATP levels in pancreatic acinar cells publication-title: Gastroenterology – volume: 1 start-page: 249 year: 2001 end-page: 267 article-title: Preservation of native properties of mitochondria in rat liver homogenate publication-title: Mitochondrion – volume: 432 start-page: 538 year: 1996 end-page: 545 article-title: Changes in element concentrations induced by agonist in pig pancreatic acinar cells publication-title: Pflugers Arch – volume: 3 start-page: 184 year: 1995 end-page: 188 article-title: Acinar cell respiration in experimental acute pancreatitis publication-title: Shock – volume: 121 start-page: 545 year: 1984 end-page: 551 article-title: The isolation and properties of mitochondria from rat pancreas publication-title: Biochem Biophys Res Commun – volume: 106 start-page: 1225 year: 1995 end-page: 1242 article-title: Na , K , and Cl transport in resting pancreatic acinar cells publication-title: J Gen Physiol – ident: e_1_2_7_31_1 doi: 10.1016/0005-2736(71)90123-4 – ident: e_1_2_7_15_1 doi: 10.1016/0005-2736(83)90001-9 – ident: e_1_2_7_22_1 doi: 10.1152/ajpendo.1978.235.5.E517 – ident: e_1_2_7_32_1 doi: 10.1073/pnas.77.6.3430 – ident: e_1_2_7_26_1 doi: 10.1042/CBI20110017 – ident: e_1_2_7_34_1 doi: 10.1016/S1567-7249(01)00025-3 – ident: e_1_2_7_3_1 doi: 10.1053/j.gastro.2010.01.037 – ident: e_1_2_7_33_1 doi: 10.1016/j.bbamem.2010.01.005 – ident: e_1_2_7_4_1 doi: 10.1016/S0021-9258(19)57191-5 – ident: e_1_2_7_5_1 doi: 10.1042/BJ20110162 – ident: e_1_2_7_30_1 doi: 10.1093/oxfordjournals.jbchem.a134941 – ident: e_1_2_7_6_1 doi: 10.1016/0006-291X(84)90216-X – ident: e_1_2_7_9_1 doi: 10.1136/gut.2007.147207 – ident: e_1_2_7_2_1 doi: 10.1097/00024382-199503000-00005 – ident: e_1_2_7_28_1 doi: 10.2337/diabetes.50.2.361 – volume: 19 start-page: 3 year: 1989 ident: e_1_2_7_8_1 article-title: Cholinergic regulation of energy exchange in mitochondria of secretory organs publication-title: Visnyk of Lviv university contributor: fullname: Shostakovska IV – ident: e_1_2_7_13_1 doi: 10.1074/jbc.M412694200 – ident: e_1_2_7_21_1 doi: 10.1113/jphysiol.1981.sp013995 – ident: e_1_2_7_25_1 doi: 10.1042/bj1920951 – ident: e_1_2_7_12_1 doi: 10.1038/nprot.2008.61 – ident: e_1_2_7_37_1 doi: 10.1161/01.CIR.0000058462.23347.93 – ident: e_1_2_7_19_1 doi: 10.1007/s004240050167 – ident: e_1_2_7_36_1 doi: 10.1016/j.febslet.2010.01.035 – ident: e_1_2_7_27_1 doi: 10.2527/2001.7951329x – ident: e_1_2_7_20_1 doi: 10.1085/jgp.106.6.1225 – volume: 255 start-page: 2457 year: 1980 ident: e_1_2_7_10_1 article-title: Inhibition of oxidative phosphorylation in ascites tumor mitochondria and cells by intramitochondrial Ca2+ publication-title: J Biol Chem doi: 10.1016/S0021-9258(19)85914-8 contributor: fullname: Villalobo A – ident: e_1_2_7_7_1 doi: 10.1023/A:1006988625831 – volume: 245 start-page: G347 year: 1983 ident: e_1_2_7_16_1 article-title: Regulation of cytosolic free Ca2+ concentration in acinar cells of rat pancreas publication-title: Am J Physiol contributor: fullname: Streb H – ident: e_1_2_7_35_1 doi: 10.1177/153537020122600606 – ident: e_1_2_7_24_1 doi: 10.1042/bj1880207 – ident: e_1_2_7_14_1 doi: 10.1152/physrev.00004.2005 – ident: e_1_2_7_11_1 doi: 10.1016/0304-4165(91)90168-G – ident: e_1_2_7_17_1 doi: 10.1074/jbc.M107794200 – ident: e_1_2_7_29_1 doi: 10.1042/bj1070807 – ident: e_1_2_7_18_1 doi: 10.1073/pnas.0904818106 – ident: e_1_2_7_23_1 doi: 10.4319/lo.1966.11.2.0264 |
| SSID | ssj0009630 |
| Score | 2.0563562 |
| Snippet | Mitochondria maintain numerous energy‐consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation... Mitochondria maintain numerous energy-consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation... |
| SourceID | proquest crossref pubmed wiley istex |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 115 |
| SubjectTerms | Acinar Cells - cytology Acinar Cells - metabolism Animals Biochemistry - methods Cell Membrane Permeability Cell Respiration KCl-based solution Kinetics Male mitochondria Mitochondria - metabolism Oxygen Consumption Pancreas - cytology Pancreas - metabolism pancreatic acinar cells Rats respiratory states in situ sucrose-based solution |
| Title | An implication of novel methodology to study pancreatic acinar mitochondria under in situ conditions |
| URI | https://api.istex.fr/ark:/67375/WNG-60M03DH7-F/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcbf.2864 https://www.ncbi.nlm.nih.gov/pubmed/22886484 https://www.proquest.com/docview/1465269431 https://search.proquest.com/docview/1314892388 |
| Volume | 31 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3di9QwEB_kRPTFj_Nr9ZQI4lv32iRtco_nnusi3D2Ihwc-hKRJYTltZbcr6l_vTNLucqIgPvWhCUlnJpPfpJPfALy0OfcElDNdhJBJ72XmFB3DuSAwHAmqiUX7Ts-qxbl8d1FeDFmVdBcm8UNsD9xoZUR_TQvcuvXhjjS0ds2U64qoQAuhKJvr5P2OOQrtajheEVkltRx5Z3N-OHa8shNdJ6F-_xPMvIpa47YzvwOfxgmnbJPL6aZ30_rnb1yO__dFd-H2gEbZcTKfe3AttPtwI9Wn_LEPN2djObj74I9bttyln7OuYW33LXxmqQZ17MH6jkW-WoY-JsHRmtmarvyyL-g60NW2Hi2e0c21FVu2bL3sNwxDcp8yxx7A-fzNh9kiG0o0ZLXkhcyK0jdWeERBuRc5r3lzhJGtUArdliiE9145Lq0ogyTmG2-dxRhGHTkpndC8Fg9hr-3a8BiYkA3PQ-URsAX03kGjuRQVzyvutC2bYgIvRnWZr4mJwyTOZW5QcoYkN4FXUY_bBnZ1SZlrqjQfz96aKj_NxclCmfkEDkZFm2HRrikKonrrCKlwrO1rFDP9Q7Ft6DbYRmD8iKBY6wk8SgayHYxzrcnWcBZRzX-dppm9ntPzyb82fAq3eCzDQblvB7DXrzbhGYKh3j2PZv8LJ1UDIA |
| link.rule.ids | 315,783,787,1378,27938,27939,46308,46732 |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Nb9MwFH8am9C48DEYdAwwEuKWLrGdxNNOo6MUWHtAm9gBybJjR6q2JahL0eCv33tx02oIJMQph9iy4_fh33Oefw_gjYm5I6AcqcT7SDonI5vTMZz1AsMRn5dt0b7xJBudyk9n6dkaHHR3YQI_xPLAjSyj9ddk4HQgvbdiDS1s2ecqk3dgA61dUPmCoy8r7ijUrMUBi4gyqWTHPBvzva7nrb1og5b1-k9A8zZubTee4QP41k055Juc9-eN7Re_fmNz_M9vegj3F4CUHQYNegRrvtqCu6FE5c8t2Bx0FeEegzus2HSVgc7qklX1D3_BQhnqtgdratZS1jJ0MwGRFswUdOuXXaL3QG9bOVR6RpfXZmxasatpM2cYlbuQPPYETofvTwajaFGlISokT2SUpK40wiEQip2IecHLfQxuRZ6j5xKJcM7llksjUi-J_MYZazCMyfetlFYoXohtWK_qyj8DJmTJY585xGweReoVakyS8TjjVpm0THrwupOX_h7IOHSgXeYaV07TyvXgbSvIZQMzO6fktTzVXycfdBaPY3E0yvWwB7udpPXCbq8oEKKS64iqcKzla1xm-o1iKl_PsY3AEBJxsVI9eBo0ZDkY50qRsuEsWjn_dZp68G5Iz51_bfgKNkcn42N9_HHy-Tnc421VDkqF24X1Zjb3LxAbNfZlawM3T10HOg |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BKx4XHuW1UMBIiFu2ju0k7rHsEpZHVwhRUakHy44daVVIqm0WAb-esZ3sqggkxCmH2LIzMx5_44y_AXiuKbMeKCcydS4R1orEFP4YzjiO4Ygr6lC073Cez47E2-PsuM-q9HdhIj_E-sDNr4zgr_0CP7P13oY0tDL1mMlcXIZtkXPq07mmHzfUUWhY_fkKT3IhxUA8S9ne0PPCVrTtpfr9TzjzImwN-055E06GGcd0k9PxqjPj6udvZI7_90m34EYPR8lBtJ_bcMk1O3AlFqj8sQPXJkM9uDtgDxqy2OSfk7YmTfvNfSGxCHXoQbqWBMJagk4m4tGK6Mrf-SVf0Xegr20smjzxV9eWZNGQ80W3IhiT25g6dheOylefJrOkr9GQVIKlIkkzW2tuEQZRyymrWL2PoS0vCvRbPOXW2sIwoXnmhKe-sdpoDGKKfSOE4ZJV_B5sNW3jHgDhombU5RYRm0P37STaS5ozmjMjdVanI3g2qEudRSoOFUmXmULJKS-5EbwIelw30MtTn7pWZOrz_LXK6SHl01mhyhHsDopW_ao992GQL7iOmArHWr9GMfufKLpx7QrbcAwgERVLOYL70UDWgzEmpbc1nEVQ81-nqSYvS_98-K8Nn8LVD9NSvX8zf_cIrrNQksPnwe3CVrdcuccIjDrzJKyAXzTABek |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=An+implication+of+novel+methodology+to+study+pancreatic+acinar+mitochondria+under+in+situ+conditions&rft.jtitle=Cell+biochemistry+and+function&rft.au=Manko%2C+Bohdan+O&rft.au=Klevets%2C+Myron+Yu&rft.au=Manko%2C+Volodymyr+V&rft.date=2013-03-01&rft.pub=Wiley+Subscription+Services%2C+Inc&rft.issn=0263-6484&rft.eissn=1099-0844&rft.volume=31&rft.issue=2&rft.spage=115&rft_id=info:doi/10.1002%2Fcbf.2864&rft.externalDBID=NO_FULL_TEXT&rft.externalDocID=3147488281 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0263-6484&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0263-6484&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0263-6484&client=summon |