Nested quantitative PCR approach for urinary cell‐free EZH2 mRNA and its potential clinical application in bladder cancer

• Published in: International journal of cancer Vol. 139; no. 8; pp. 1830 - 1838
• Main Authors: Zhang, Xin, Zhang, Yanli, Liu, Xinfeng, Liu, Tong, Li, Peilong, Du, Lutao, Yang, Yongmei, Wang, Lili, Wang, Chuanxin
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 15.10.2016
• Subjects: Aged; Aged, 80 and over; Biomarkers; Bladder cancer; Cancer; Case-Control Studies; cell‐free; Enhancer of Zeste Homolog 2 Protein - genetics; EZH2 mRNA; Female; Humans; Lymphatic Metastasis; Male; Medical prognosis; Medical research; Middle Aged; Neoplasm Staging; Polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - urine; Urinary Bladder Neoplasms - enzymology; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - pathology; Urinary Bladder Neoplasms - urine; Urine; cell-free; urine; EZH2 mRNA; bladder cancer

EZH2 is overexpressed in bladder cancer (BC) and plays important roles in tumor development and progression. Recent studies show cell free (cf) RNAs released from cancer cells can reflect tissues changes and are stable and detectable in urine. Although conventional quantitative real‐time PCR (qPCR)...