Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins

• Published in: Frontiers in chemistry Vol. 11; p. 1328081
• Main Authors: Krueger, Taylor D, Henderson, J Nathan, Breen, Isabella L, Zhu, Liangdong, Wachter, Rebekka M, Mills, Jeremy H, Fang, Chong
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 07.12.2023
• Subjects: Chemistry; femtosecond stimulated Raman; photoconvertible fluorescent proteins; photoswitchable fluorescent proteins; structural dynamics; ultrafast spectroscopy; X-ray crystallography; X-ray crystallography; photoswitchable fluorescent proteins; structural dynamics; ultrafast spectroscopy; photoconvertible fluorescent proteins; biophysical chemistry and biomolecules; femtosecond stimulated Raman

Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes for sophisticated and advanced bioimaging techniques. Among them, an interplay between photoswitching and photoconversion has only been observed in a limited subset of Kaede-like FPs that show potential for disco...