Granulocytic differentiation of HL-60 promyelocytic leukemia cells is associated with increased expression of Cul5

• Published in: In vitro cellular & developmental biology. Animal Vol. 45; no. 5-6; pp. 264 - 274
• Main Authors: Baxter, Shaneen S, Carlson, Lauren A, Mayer, Alejandro M. S, Hall, Mary L, Fay, Michael J
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.05.2009; Springer Science + Business Media; Springer-Verlag
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Blotting, Western; Cell Biology; Cell Count; Cell Culture; Cell Differentiation - drug effects; Cell Growth/Differentiation/Apoptosis; Cell lines; Cell Membrane - drug effects; Cell Membrane - metabolism; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cell Survival - drug effects; Cullin Proteins - metabolism; Developmental Biology; Elongin; Gene Expression Regulation, Leukemic - drug effects; Genes; Granulocytes - cytology; Granulocytes - drug effects; Hematopoietic stem cells; HL-60 Cells; Humans; Isotypes; Leukemia, Promyelocytic, Acute - genetics; Leukemia, Promyelocytic, Acute - pathology; Life Sciences; Messenger RNA; Myeloid cells; Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem Cells; Superoxides - metabolism; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; Surface antigens; Tetradecanoylphorbol Acetate - pharmacology; Transcription Factors - genetics; Transcription Factors - metabolism; Tretinoin - pharmacology; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitins; Granulocytic differentiation; All; retinoic acid; Elongin B/C; HL-60; ROC2; Cul5; ASB-2

The human HL-60 promyelocytic leukemia cell line has been widely used as a model for studying granulocytic differentiation. All-trans retinoic acid (ATRA) treatment of HL-60 cells promotes granulocytic differentiation and is effective as differentiation therapy for patients with acute promyelocytic...