NMR of paramagnetic metalloproteins in solution: Ubi venire, quo vadis?

• Published in: Journal of inorganic biochemistry Vol. 234; p. 111871
• Main Authors: Trindade, Inês B., Coelho, Anaísa, Cantini, Francesca, Piccioli, Mario, Louro, Ricardo O.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2022
• Subjects: Electronic structure; Metal homeostasis and trafficking; Metalloproteins; NMR spectroscopy; Paramagnetic; Structural biology; Electronic structure; Metal homeostasis and trafficking; NMR spectroscopy; Metalloproteins; Structural biology; Paramagnetic

Metalloproteins represent a substantial fraction of the proteome where they have an outsized contribution to enzymology. This stems from the reactivity of transition metals found in the active sites of numerous classes of enzymes that undergo redox and/or spin-state transitions. Notwithstanding, NMR...