Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells
We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (>13,000 sites/cell) than intrinsic human IL-18 recep...
Saved in:
| Published in | Journal of immunological methods Vol. 217; no. 1; pp. 97 - 102 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Amsterdam
Elsevier B.V
01.08.1998
Elsevier |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (>13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (<2,500 sites/cell). And the cells responded to MuIL-18 as well as to HuIL-18 in a dose-dependent manner, and produced large amounts of interferon-γ (IFN-γ). We could estimate the amount of murine IL-18 based on the amounts of IFN-γ produced by these cells. The stoichiometry was observed up to 150 ng/ml of MuIL-18. By using these cells, a large amount of MuIL-18 (448±89.2 ng/ml) was detected in sera of
Propionibacterium acnes (
P. acnes)/lipopolysaccharide (LPS)-treated endotoxic mice (the same conditions in which IL-18 was first identified). These cells provide us with a useful tool for determining the bioactivity of MuIL-18. |
|---|---|
| AbstractList | We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (>13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (<2,500 sites/cell). And the cells responded to MuIL-18 as well as to HuIL-18 in a dose-dependent manner, and produced large amounts of interferon-γ (IFN-γ). We could estimate the amount of murine IL-18 based on the amounts of IFN-γ produced by these cells. The stoichiometry was observed up to 150 ng/ml of MuIL-18. By using these cells, a large amount of MuIL-18 (448±89.2 ng/ml) was detected in sera of
Propionibacterium acnes (
P. acnes)/lipopolysaccharide (LPS)-treated endotoxic mice (the same conditions in which IL-18 was first identified). These cells provide us with a useful tool for determining the bioactivity of MuIL-18. We genetically engineered human myelomonocytic KG-I cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (> 13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (< 2,500 sites/cell). And the cells responded to MuIL-18 as well as to HuIL-18 in a dose-dependent manner, and produced large amounts of interferon-gamma (IFN-gamma). We could estimate the amount of murine IL-18 based on the amounts of IFN-gamma produced by these cells. The stoichiometry was observed up to 150 ng/ml of MuIL-18. By using these cells, a large amount of MuIL-18 (448 +/- 89.2 ng/ml) was detected in sera of Propionibacterium acnes (P. acnes)/lipopolysaccharide (LPS)-treated endotoxic mice (the same conditions in which IL-18 was first identified). These cells provide us with a useful tool for determining the bioactivity of MuIL-18. We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (>13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (<2,500 sites/cell). And the cells responded to MuIL-18 as well as to HuIL-18 in a dose-dependent manner, and produced large amounts of interferon- gamma (IFN- gamma ). We could estimate the amount of murine IL-18 based on the amounts of IFN- gamma produced by these cells. The stoichiometry was observed up to 150 ng/ml of MuIL-18. By using these cells, a large amount of MuIL-18 (448 plus or minus 89.2 ng/ml) was detected in sera of Propionibacterium acnes (P. acnes) /lipopolysaccharide (LPS)-treated endotoxic mice (the same conditions in which IL-18 was first identified). These cells provide us with a useful tool for determining the bioactivity of MuIL-18. |
| Author | Yamauchi, Hiroshi Mori, Tsutomu Kurimoto, Masashi Ushio, Shimpei Taniguchi, Mutsuko Nagaoka, Katsue Okura, Takanori Ohta, Tsunetaka Nukada, Yoshiyuki Ikegami, Hakuo |
| Author_xml | – sequence: 1 givenname: Mutsuko surname: Taniguchi fullname: Taniguchi, Mutsuko – sequence: 2 givenname: Katsue surname: Nagaoka fullname: Nagaoka, Katsue – sequence: 3 givenname: Shimpei surname: Ushio fullname: Ushio, Shimpei – sequence: 4 givenname: Yoshiyuki surname: Nukada fullname: Nukada, Yoshiyuki – sequence: 5 givenname: Takanori surname: Okura fullname: Okura, Takanori – sequence: 6 givenname: Tsutomu surname: Mori fullname: Mori, Tsutomu – sequence: 7 givenname: Hiroshi surname: Yamauchi fullname: Yamauchi, Hiroshi – sequence: 8 givenname: Tsunetaka surname: Ohta fullname: Ohta, Tsunetaka – sequence: 9 givenname: Hakuo surname: Ikegami fullname: Ikegami, Hakuo – sequence: 10 givenname: Masashi surname: Kurimoto fullname: Kurimoto, Masashi |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2416488$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/9776579$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkc1u1DAUhS1UVKaFR6jkBUKwCLU9iX9WqCqlrVrBAlhbtnPNGBJ7aieV8i48LElnNLuK1V2c7957dM4JOoopAkJnlHykhPLz74QwVlHRqPdKfiCEKFmxF2hFpWCVUKQ5QqsD8gqdlPJ7hijh5BgdKyF4I9QK_b0qg7FdKJse4oCTx8MGsIOuK3gs4McO-5RxP-YQAYc4QO5g_BNiRSW2IZlSzITttEg5taML8dczdAYH22E-5j5_vVjEhDdjbyLuJ-hSn2Jy0xAcvruu6M7Ba_TSm67Am_08RT-_XP24vKnuv13fXl7cV65mZKiANIRK5UndSm6JamRjW-pYS2wjqVqb2jvhJeOq9pwJbxhR3nJuBfPCtnx9it7t7m5zehihDLoPZXFgIqSxaMqb9ZrwegabHehyKiWD19scepMnTYleWtFPreglcq2kfmpFs3nvbP9gtD20h619DbP-dq-b4kzns4kulAPGasprKWfs0w6DOYzHAFkXFyA6aMMc7qDbFP5j5B_kBaz7 |
| CODEN | JIMMBG |
| CitedBy_id | crossref_primary_10_1073_pnas_96_20_11537 crossref_primary_10_1097_00001432_200106000_00004 crossref_primary_10_1016_j_jim_2007_07_002 crossref_primary_10_1002_0471142735_im0626s44 crossref_primary_10_1038_sj_leu_2401789 crossref_primary_10_1248_bpb_29_896 |
| Cites_doi | 10.1006/bbrc.1997.7099 10.4049/jimmunol.157.9.3967 10.1093/nar/18.17.5322 10.1006/cimm.1996.0272 10.1006/bbrc.1997.6665 10.1074/jbc.271.8.3967 10.1006/clin.1997.4475 10.1074/jbc.272.41.25737 10.1007/s002620050345 10.1002/eji.1830260736 10.4049/jimmunol.156.11.4274 10.1016/S0022-1759(97)00164-6 10.4049/jimmunol.158.4.1541 10.1006/bbrc.1998.8236 10.1038/378088a0 |
| ContentType | Journal Article |
| Copyright | 1998 Elsevier Science B.V. 1998 INIST-CNRS |
| Copyright_xml | – notice: 1998 Elsevier Science B.V. – notice: 1998 INIST-CNRS |
| DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QO 7T5 8FD FR3 H94 P64 |
| DOI | 10.1016/S0022-1759(98)00098-2 |
| DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Biotechnology Research Abstracts Immunology Abstracts Technology Research Database Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef AIDS and Cancer Research Abstracts Immunology Abstracts Engineering Research Database Biotechnology Research Abstracts Technology Research Database Biotechnology and BioEngineering Abstracts |
| DatabaseTitleList | MEDLINE AIDS and Cancer Research Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1872-7905 |
| EndPage | 102 |
| ExternalDocumentID | 10_1016_S0022_1759_98_00098_2 9776579 2416488 S0022175998000982 |
| Genre | Journal Article |
| GroupedDBID | --- --K --M .55 .GJ .~1 0R~ 1B1 1RT 1~. 1~5 29K 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 8P~ 9JM AAAJQ AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXUO ABEFU ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHHHB AHPSJ AI. AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CJTIS CNWQP CS3 D-I DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HMG HVGLF HZ~ IH2 IHE J1W K-O KOM L7B LUGTX M41 MO0 N9A O-L O9- OAUVE OHT OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SDF SDG SDP SES SEW SIN SPCBC SSI SSU SSZ T5K UAP VH1 WUQ X7M XFK XPP Y6R ZGI ZMT ~G- 08R AAUGY ABPIF ABPTK IQODW AAHBH AAXKI AKRWK CGR CUY CVF ECM EIF NPM AAYXX AFJKZ CITATION 7QO 7T5 8FD FR3 H94 P64 |
| ID | FETCH-LOGICAL-c420t-e050189f04d86b09585bd1c2d0b58193a4fc7f82694f627fa209fb66b72f7bd63 |
| IEDL.DBID | .~1 |
| ISSN | 0022-1759 |
| IngestDate | Fri Oct 25 05:34:26 EDT 2024 Thu Sep 26 18:09:42 EDT 2024 Sat Sep 28 08:37:54 EDT 2024 Sun Oct 29 17:07:08 EDT 2023 Fri Feb 23 02:33:50 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | IL-18R, interleukin-18 receptor Bioassay Murine IL-18 cDNA, complementary DNA Cytokine IFNγ, interferon-γ Recombinant DNA IL-18, interleukin-18 Human cells Human Cell line Transfection Established cell line Bone marrow Biosynthesis Gamma interferon Biological receptor Genetic transfer |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c420t-e050189f04d86b09585bd1c2d0b58193a4fc7f82694f627fa209fb66b72f7bd63 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| PMID | 9776579 |
| PQID | 16533064 |
| PQPubID | 23462 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_16533064 crossref_primary_10_1016_S0022_1759_98_00098_2 pubmed_primary_9776579 pascalfrancis_primary_2416488 elsevier_sciencedirect_doi_10_1016_S0022_1759_98_00098_2 |
| PublicationCentury | 1900 |
| PublicationDate | 1998-08-01 |
| PublicationDateYYYYMMDD | 1998-08-01 |
| PublicationDate_xml | – month: 08 year: 1998 text: 1998-08-01 day: 01 |
| PublicationDecade | 1990 |
| PublicationPlace | Amsterdam |
| PublicationPlace_xml | – name: Amsterdam – name: Netherlands |
| PublicationTitle | Journal of immunological methods |
| PublicationTitleAlternate | J Immunol Methods |
| PublicationYear | 1998 |
| Publisher | Elsevier B.V Elsevier |
| Publisher_xml | – name: Elsevier B.V – name: Elsevier |
| References | Kojima, Takeuchi, Ohta, Nishida, Arai, Ikeda, Ikegami, Kurimoto (BIB5) 1998; 244 Dao, Ohashi, Kayano, Kurimoto, Okamura (BIB1) 1996; 173 Matsumoto, Tsuji-Takayama, Aizawa, Koide, Ohta, Kurimoto (BIB6) 1997; 234 Tsutsui, Nakanishi, Matsui, Higashino, Okamura, Miyazawa, Kaneda (BIB16) 1996; 157 Torigoe, Ushio, Okura, Kobayashi, Taniai, Kunikata, Murakami, Sanou, Kojima, Fujii, Ohta, Ikeda, Ikegami, Kurimoto (BIB14) 1997; 272 Mizushima, Nagata (BIB10) 1990; 18 Tsuji-Takayama, Matsumoto, Koide, Takeuchi, Ikeda, Ohta, Kurimoto (BIB15) 1997; 237 Micallef, Yoshida, Kawai, Hanaya, Kohno, Arai, Tanimoto, Torigoe, Fujii, Ikeda, Kurimoto (BIB8) 1997; 43 Micallef, Tanimoto, Kohno, Ikeda, Kurimoto (BIB9) 1997; 57 Kohno, Kataoka, Ohtsuki, Suemoto, Okamoto, Usui, Ikeda, Kurimoto (BIB4) 1997; 158 Ohtsuki, Micallef, Kohno, Tanimoto, Ikeda, Kurimoto (BIB11) 1997; 17 Ushio, Namba, Okura, Hattori, Nukada, Akita, Tanabe, Konishi, Micallef, Fujii, Torigoe, Tanimoto, Fukuda, Ikeda, Okamura, Kurimoto (BIB17) 1996; 156 Parnet, Garka, Bonner, Dower, Sims (BIB13) 1996; 271 Micallef, Ohtsuki, Kohno, Tanabe, Ushio, Namba, Torigoe, Fujii, Ikeda, Fukuda, Kurimoto (BIB7) 1996; 26 Konishi, Tanabe, Taniguchi, Yamauchi, Tanimoto, Ikeda, Orita, Kurimoto (BIB2) 1997; 209 Kohno, Kurimoto (BIB3) 1998; 86 Okamura, Tsutsui, Komatsu, Yutsudo, Hakura, Tanimoto, Torigoe, Okura, Nukada, Hattori, Akita, Namba, Tanabe, Konishi, Fukuda, Kurimoto (BIB12) 1995; 378 Kojima (10.1016/S0022-1759(98)00098-2_BIB5) 1998; 244 Kohno (10.1016/S0022-1759(98)00098-2_BIB3) 1998; 86 Tsuji-Takayama (10.1016/S0022-1759(98)00098-2_BIB15) 1997; 237 Micallef (10.1016/S0022-1759(98)00098-2_BIB8) 1997; 43 Konishi (10.1016/S0022-1759(98)00098-2_BIB2) 1997; 209 Micallef (10.1016/S0022-1759(98)00098-2_BIB7) 1996; 26 Mizushima (10.1016/S0022-1759(98)00098-2_BIB10) 1990; 18 Parnet (10.1016/S0022-1759(98)00098-2_BIB13) 1996; 271 Torigoe (10.1016/S0022-1759(98)00098-2_BIB14) 1997; 272 Dao (10.1016/S0022-1759(98)00098-2_BIB1) 1996; 173 Tsutsui (10.1016/S0022-1759(98)00098-2_BIB16) 1996; 157 Ohtsuki (10.1016/S0022-1759(98)00098-2_BIB11) 1997; 17 Kohno (10.1016/S0022-1759(98)00098-2_BIB4) 1997; 158 Okamura (10.1016/S0022-1759(98)00098-2_BIB12) 1995; 378 Ushio (10.1016/S0022-1759(98)00098-2_BIB17) 1996; 156 Matsumoto (10.1016/S0022-1759(98)00098-2_BIB6) 1997; 234 Micallef (10.1016/S0022-1759(98)00098-2_BIB9) 1997; 57 |
| References_xml | – volume: 173 start-page: 230 year: 1996 ident: BIB1 article-title: Interferon-γ-inducing factor, a novel cytokine, enhances Fas ligand-mediated cytotoxicity of murine T helper 1 cells publication-title: Cell. Immunol. contributor: fullname: Okamura – volume: 209 start-page: 187 year: 1997 ident: BIB2 article-title: A simple and sensitive bioassay for the detection of human interleukin-18/interferon-γ-inducing factor using human myelomonocytic KG-1 cells publication-title: J. Immunol. Meth. contributor: fullname: Kurimoto – volume: 18 start-page: 5322 year: 1990 ident: BIB10 article-title: pEF-BOS, a powerful mammalian expression vector publication-title: Nucleic Acids Res. contributor: fullname: Nagata – volume: 237 start-page: 126 year: 1997 ident: BIB15 article-title: Interleukin-18 induces activation and association of p56lck and MAPK in a murine TH1 clone publication-title: Biochem. Biophys. Res. Commun. contributor: fullname: Kurimoto – volume: 17 start-page: 3253 year: 1997 ident: BIB11 article-title: Interleukin 18 enhances Fas ligand expression and induces apoptosis in Fas-expressing human myelomonocytic KG-1 cells publication-title: Anticancer Res. contributor: fullname: Kurimoto – volume: 43 start-page: 361 year: 1997 ident: BIB8 article-title: In vivo antitumor effects of murine interferon-γ-inducing factor/interleukin-18 in mice bearing syngeneic Meth A malignant ascites publication-title: Cancer Immunol. Immunother. contributor: fullname: Kurimoto – volume: 156 start-page: 4274 year: 1996 ident: BIB17 article-title: Cloning of the cDNA for human IFN-γ-inducing factor, expression in publication-title: J. Immunol. contributor: fullname: Kurimoto – volume: 271 start-page: 3967 year: 1996 ident: BIB13 article-title: IL-1 Rrp is a novel receptor-like molecule similar to the type I interleukin-1 receptor and its homologues T1/ST2 and IL-1R Acp publication-title: J. Biol. Chem. contributor: fullname: Sims – volume: 272 start-page: 25737 year: 1997 ident: BIB14 article-title: Purification and characterization of the human interleukin-18 receptor publication-title: J. Biol. Chem. contributor: fullname: Kurimoto – volume: 378 start-page: 88 year: 1995 ident: BIB12 article-title: Cloning of a new cytokine that induces IFN-γ production by T cells publication-title: Nature contributor: fullname: Kurimoto – volume: 57 start-page: 4557 year: 1997 ident: BIB9 article-title: Interleukin 18 induces the sequential activation of natural killer cells and cytotoxic T lymphocytes to protect syngeneic mice from transplantation with Meth A sarcoma publication-title: Cancer Res. contributor: fullname: Kurimoto – volume: 157 start-page: 3967 year: 1996 ident: BIB16 article-title: IFN-γ-inducing factor up-regulates Fas ligand-mediated cytotoxic activity of murine natural killer cell clones publication-title: J. Immunol. contributor: fullname: Kaneda – volume: 158 start-page: 1541 year: 1997 ident: BIB4 article-title: IFN-γ-inducing factor (IGIF) is a costimulatory factor on the activation of Th1 but not Th2 cells and exerts its effect independently of IL-12 publication-title: J. Immunol. contributor: fullname: Kurimoto – volume: 234 start-page: 454 year: 1997 ident: BIB6 article-title: Interleukin-18 activates NF-κB in murine T helper type 1 cells publication-title: Biochem. Biophys. Res. Commun. contributor: fullname: Kurimoto – volume: 244 start-page: 183 year: 1998 ident: BIB5 article-title: Interleukin-18 activates the IRAK-TRAF6 pathway in mouse EL-4 cells publication-title: Biochem. Biophys. Res. Commun. contributor: fullname: Kurimoto – volume: 86 start-page: 11 year: 1998 ident: BIB3 article-title: Interleukin 18, a cytokine which resembles IL-1 structurally and IL-12 functionally but exerts its effect independently of both publication-title: Clin. Immunol. Immunopathol. contributor: fullname: Kurimoto – volume: 26 start-page: 1647 year: 1996 ident: BIB7 article-title: Interferon-γ-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-γ production publication-title: Eur. J. Immunol. contributor: fullname: Kurimoto – volume: 237 start-page: 126 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB15 article-title: Interleukin-18 induces activation and association of p56lck and MAPK in a murine TH1 clone publication-title: Biochem. Biophys. Res. Commun. doi: 10.1006/bbrc.1997.7099 contributor: fullname: Tsuji-Takayama – volume: 157 start-page: 3967 year: 1996 ident: 10.1016/S0022-1759(98)00098-2_BIB16 article-title: IFN-γ-inducing factor up-regulates Fas ligand-mediated cytotoxic activity of murine natural killer cell clones publication-title: J. Immunol. doi: 10.4049/jimmunol.157.9.3967 contributor: fullname: Tsutsui – volume: 18 start-page: 5322 year: 1990 ident: 10.1016/S0022-1759(98)00098-2_BIB10 article-title: pEF-BOS, a powerful mammalian expression vector publication-title: Nucleic Acids Res. doi: 10.1093/nar/18.17.5322 contributor: fullname: Mizushima – volume: 173 start-page: 230 year: 1996 ident: 10.1016/S0022-1759(98)00098-2_BIB1 article-title: Interferon-γ-inducing factor, a novel cytokine, enhances Fas ligand-mediated cytotoxicity of murine T helper 1 cells publication-title: Cell. Immunol. doi: 10.1006/cimm.1996.0272 contributor: fullname: Dao – volume: 57 start-page: 4557 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB9 article-title: Interleukin 18 induces the sequential activation of natural killer cells and cytotoxic T lymphocytes to protect syngeneic mice from transplantation with Meth A sarcoma publication-title: Cancer Res. contributor: fullname: Micallef – volume: 234 start-page: 454 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB6 article-title: Interleukin-18 activates NF-κB in murine T helper type 1 cells publication-title: Biochem. Biophys. Res. Commun. doi: 10.1006/bbrc.1997.6665 contributor: fullname: Matsumoto – volume: 271 start-page: 3967 year: 1996 ident: 10.1016/S0022-1759(98)00098-2_BIB13 article-title: IL-1 Rrp is a novel receptor-like molecule similar to the type I interleukin-1 receptor and its homologues T1/ST2 and IL-1R Acp publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.8.3967 contributor: fullname: Parnet – volume: 86 start-page: 11 year: 1998 ident: 10.1016/S0022-1759(98)00098-2_BIB3 article-title: Interleukin 18, a cytokine which resembles IL-1 structurally and IL-12 functionally but exerts its effect independently of both publication-title: Clin. Immunol. Immunopathol. doi: 10.1006/clin.1997.4475 contributor: fullname: Kohno – volume: 17 start-page: 3253 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB11 article-title: Interleukin 18 enhances Fas ligand expression and induces apoptosis in Fas-expressing human myelomonocytic KG-1 cells publication-title: Anticancer Res. contributor: fullname: Ohtsuki – volume: 272 start-page: 25737 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB14 article-title: Purification and characterization of the human interleukin-18 receptor publication-title: J. Biol. Chem. doi: 10.1074/jbc.272.41.25737 contributor: fullname: Torigoe – volume: 43 start-page: 361 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB8 article-title: In vivo antitumor effects of murine interferon-γ-inducing factor/interleukin-18 in mice bearing syngeneic Meth A malignant ascites publication-title: Cancer Immunol. Immunother. doi: 10.1007/s002620050345 contributor: fullname: Micallef – volume: 26 start-page: 1647 year: 1996 ident: 10.1016/S0022-1759(98)00098-2_BIB7 article-title: Interferon-γ-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-γ production publication-title: Eur. J. Immunol. doi: 10.1002/eji.1830260736 contributor: fullname: Micallef – volume: 156 start-page: 4274 year: 1996 ident: 10.1016/S0022-1759(98)00098-2_BIB17 article-title: Cloning of the cDNA for human IFN-γ-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein publication-title: J. Immunol. doi: 10.4049/jimmunol.156.11.4274 contributor: fullname: Ushio – volume: 209 start-page: 187 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB2 article-title: A simple and sensitive bioassay for the detection of human interleukin-18/interferon-γ-inducing factor using human myelomonocytic KG-1 cells publication-title: J. Immunol. Meth. doi: 10.1016/S0022-1759(97)00164-6 contributor: fullname: Konishi – volume: 158 start-page: 1541 year: 1997 ident: 10.1016/S0022-1759(98)00098-2_BIB4 article-title: IFN-γ-inducing factor (IGIF) is a costimulatory factor on the activation of Th1 but not Th2 cells and exerts its effect independently of IL-12 publication-title: J. Immunol. doi: 10.4049/jimmunol.158.4.1541 contributor: fullname: Kohno – volume: 244 start-page: 183 year: 1998 ident: 10.1016/S0022-1759(98)00098-2_BIB5 article-title: Interleukin-18 activates the IRAK-TRAF6 pathway in mouse EL-4 cells publication-title: Biochem. Biophys. Res. Commun. doi: 10.1006/bbrc.1998.8236 contributor: fullname: Kojima – volume: 378 start-page: 88 year: 1995 ident: 10.1016/S0022-1759(98)00098-2_BIB12 article-title: Cloning of a new cytokine that induces IFN-γ production by T cells publication-title: Nature doi: 10.1038/378088a0 contributor: fullname: Okamura |
| SSID | ssj0001060 |
| Score | 1.6380445 |
| Snippet | We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which... We genetically engineered human myelomonocytic KG-I cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which... |
| SourceID | proquest crossref pubmed pascalfrancis elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 97 |
| SubjectTerms | Animal cells Animals Bioassay Biological and medical sciences Biological Assay Cell cultures. Hybridization. Fusion Cytokine DNA, Complementary - genetics Endotoxemia - blood Endotoxemia - chemically induced Endotoxemia - immunology Fundamental and applied biological sciences. Psychology Genetic Vectors Human cells Humans Interferon-gamma - biosynthesis Interleukin-18 - analysis Interleukin-18 - metabolism Interleukin-18 Receptor alpha Subunit Lipopolysaccharides - toxicity Mice Mice, Inbred C57BL Molecular and cellular biology Monocytes - metabolism Murine IL-18 Propionibacterium acnes Protein Binding Receptors, Interleukin - genetics Receptors, Interleukin - metabolism Receptors, Interleukin-18 Recombinant DNA Recombinant Fusion Proteins - metabolism Signal Transduction Tumor Cells, Cultured |
| Title | Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells |
| URI | https://dx.doi.org/10.1016/S0022-1759(98)00098-2 https://www.ncbi.nlm.nih.gov/pubmed/9776579 https://search.proquest.com/docview/16533064 |
| Volume | 217 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB4hUKtKVdXSom4L1Ice2oMhcRLHOa4odFvEnorEzYqztrRiSVbs5pBLf0l_bGfshBWHFRLX-JGRZzLzjTMPgK_Klg5dWURumSh4WomEG-EMt6kq0piiAhzlDl9N5eQ6_X2T3ezA2ZALQ2GVve4POt1r6_7JaX-ap8v5nHJ8BeLpDP0FAgqK9DAV20KZPvm7CfNAlycaKobT7E0WT9jBP_xWqO9-Ey622afXy3KFp-ZCu4vteNTbpYu38KYHlGwcaH4HO7behxehxWS3Dy-v-p_n7-HfOSJBf-lEN4KscQzBH6Ob-xVrV9a1C4YIlt3R_btlVEfifmHb23nNY8XMvEGYXXbMdDTk68Si1dsyG4_ULtGbZ9WP6ZgGG-abAbK7zi4alPym6pBedvmTx4GCD3B9cf7nbML75gy8SkW05jaiUoCFi9KZkgaBmsrMLK7ELDIZooykTF2VO0WJsk6K3JUiKpyR0uTC5WYmkwPYrZvafgSWVElWVDiYG0fl28oMtY6VsTSobKpSjuBkYIlehhocehOchjzUxENdKO15qMUI1MA4_UiYNNqJp5YePWL0wwsR6UhUdiP4MjBe44dIJ1TWtmlXOpYUqCvTERwEeXhYihBbZnnx6flUfYZXIReSAg8PYXd939ojBENrc-yl_Rj2xr8uJ9P_FpoEkw |
| link.rule.ids | 315,783,787,4511,24130,27938,27939,45599,45693 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1BT9swFLYQiA1pmjY2RLcxfNhhO3gkTuLYx4rBCqU9gcTNil1bqihJRZtD_st-7N6LkyIO1aRd7dix3nv5_PnlvWdCvklXeDjKAnPLuGKp5Qkz3BvmUqnSGKMCPOYOT6ZidJde32f3O-S8z4XBsMoO-wOmt2jdtZx10jxbzueY48uBT2dwXkCiIAGH94ANKDD2veHVeDTdADKceqK-aDgOeE7kCZO0jd-V_NHOw_i2LerNsliB4Hy48WI7JW23pst35G3HKekwLPs92XHlIdkPt0w2h-TVpPt__oH8uQAy2Pqd0ClIK0-B_1F03q9ovXK-XlAgsfQRXfCOYimJp4WrH-YliyU18wqYdtFQ02BXWyoWNr4tT4NU3RIO9NT-mg6xs6LtfYD0sXGLCoy_sg2sl45_szis4CO5u7y4PR-x7n4GZlMerZmLsBqg8lE6k8IAV5OZmcWWzyKTAdFIitTb3EvMlfWC577gkfJGCJNzn5uZSI7IblmV7pjQxCaZstCZG48V3IoMgMeJWBjAG1uIAfnZq0QvQxkO_RyfBjrUqEOtpG51qPmAyF5x-oU9adgq_jX05IWiNy8EsiMA7wbktFe8hm8RJVSUrqpXOhYYqyvSATkK9rAZCixbZLn69P-rOiWvR7eTG31zNR1_JgchNRLjEL-Q3fVT7U6AG63N1872_wIpowdQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Establishment+of+the+cells+useful+for+murine+interleukin-18+bioassay+by+introducing+murine+interleukin-18+receptor+cDNA+into+human+myelomonocytic+KG-1+cells&rft.jtitle=Journal+of+immunological+methods&rft.au=Taniguchi%2C+Mutsuko&rft.au=Nagaoka%2C+Katsue&rft.au=Ushio%2C+Shimpei&rft.au=Nukada%2C+Yoshiyuki&rft.date=1998-08-01&rft.pub=Elsevier+B.V&rft.issn=0022-1759&rft.eissn=1872-7905&rft.volume=217&rft.issue=1&rft.spage=97&rft.epage=102&rft_id=info:doi/10.1016%2FS0022-1759%2898%2900098-2&rft.externalDocID=S0022175998000982 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-1759&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-1759&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-1759&client=summon |