Interleukin-2 inhibits HIV-1 replication in human macrophages by modulating expression of CD4 and CC-chemokine receptor-5

• Published in: AIDS (London) Vol. 12; no. 8; pp. F59 - F64
• Main Authors: KUTZA, J, HAYES, M. R, CLOUSE, K. A
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 28.05.1998
• Subjects: AIDS/HIV; Biological and medical sciences; CD4 Antigens - biosynthesis; Cells, Cultured; Chemokines - biosynthesis; Cytokines - biosynthesis; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; HIV Reverse Transcriptase - antagonists & inhibitors; HIV-1 - physiology; Human viral diseases; Humans; Infectious diseases; Interleukin-2 - pharmacology; Macrophage Colony-Stimulating Factor - biosynthesis; Macrophages - metabolism; Macrophages - virology; Medical sciences; Monocytes - metabolism; Monocytes - virology; Receptors, CCR5 - biosynthesis; Recombinant Proteins - pharmacology; Viral diseases; Viral hepatitis; Virus Replication; Human; Immunopathology; HIV-1 virus; Cytokine; Retroviridae; AIDS; Immune deficiency; Lentivirus; In vitro; Biological activity; Infection; Virus; Chemokine; Immunostimulant agent; Interleukin 2; Viral disease; Immunotherapy; Production; Human immunodeficiency virus; Infected cell; Macrophage colony stimulating factor; Macrophage
• ISSN: 0269-9370; 1473-5571
• DOI: 10.1097/00002030-199808000-00001

To determine the effect of recombinant human interleukin (IL)-2 on HIV-1 replication and macrophage colony stimulating factor (M-CSF) production by HIV-1-infected monocyte-derived macrophages (MDM). Therapeutic use of IL-2 increases the number and function of CD4+ T cells. IL-2 also increases M-CSF production and M-CSF receptor expression by human monocytes, but the subsequent effects on HIV-1 replication in MDM have yet to be determined. MDM from HIV-1-seronegative donors were cultured in the presence and absence of IL-2 and infected with HIV-1. Harvested supernatants were monitored for reverse transcriptase activity and M-CSF production. Reverse transcriptase activity was significantly lower when MDM cultures were treated with IL-2 for 10 days prior to infection with HIV-1. IL-2 did not stimulate production of inhibitory chemokines or cytokines, but FACS analysis revealed that expression of CD4, the primary HIV-1 receptor, and CC-chemokine receptor-5, a coreceptor used by macrophage-tropic viruses, are down modulated after treatment with IL-2. IL-2 may not only be of benefit in restoring immune function in AIDS patients, but may also help to prevent the infection of healthy macrophages by decreasing their expression of HIV-1 receptors.