Decreased endothelial cell glutathione and increased sensitivity to oxidative stress in an in vitro blood–brain barrier model system

• Published in: Brain research Vol. 802; no. 1; pp. 232 - 240
• Main Authors: Hurst, Roger D, Heales, Simon J.R, Dobbie, Michael S, Barker, Jane E, Clark, John B
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 17.08.1998; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Blood-Brain Barrier - physiology; Blood–brain barrier; Buthionine Sulfoximine - pharmacology; Cell Death - physiology; Cells, Cultured; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Drug Resistance; Electric Impedance; Endothelial cell; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Endothelium, Vascular - physiology; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Glutathione; Glutathione - metabolism; Humans; L-Lactate Dehydrogenase - metabolism; Nitric oxide; Nitric Oxide - pharmacology; Oxidative stress; Oxidative Stress - physiology; Rats; Rats, Wistar; Transendothelial resistance; Vertebrates: nervous system and sense organs; Oxidative stress; GSH, reduced glutathione; Endothelial cell; TNF-α, tumour necrosis factor-α; TEER, transendothelial electrical resistance; LDH, lactate dehydrogenase; l-BSO, l-buthionine sulfoximine; PBS, phosphate buffered saline; BBB, blood–brain barrier; Blood–brain barrier; NO, nitric oxide; Nitric oxide; Transendothelial resistance; Glutathione; Sensitivity; Central nervous system; Models; Blood brain barrier; In vitro; Brain (vertebrata)

Using a cell culture model of the blood–brain barrier (BBB) we have evaluated the role of endothelial cell glutathione in protecting barrier integrity against nitric oxide (NO)-induced oxidative stress. The co-culture of human umbilical vein endothelial cells (ECV304) with rat (C6) glioma cells, or...