Effect of nitric oxide synthase inhibitors in acute lung injury due to blunt lung trauma in rats

• Published in: Türk göğüs kalp damar cerrahisi dergisi Vol. 27; no. 1; pp. 63 - 72
• Main Authors: Akgül, Aslı Gül, Şahin, Deniz, Temel, Uğur, Eliçora, Aykut, Dillioğlugil, Meltem, Maral Kır, Hale, Özsoy, Özgür Doğa, Yıldız, Kürşat, Özer, Cüneyt, Topçu, Salih
• Format: Journal Article
• Language: English
• Published: Turkey Bayçınar Medical Publishing 01.01.2019
• Subjects: Original; trauma; Acute lung injury; oxidative stress
• DOI: 10.5606/TGKDC.DERGISI.2019.15936
• ISSN: 1301-5680

This study aims to investigate the effects of blunt lung trauma performed in experimental rat model on lung tissue and blood as well as proinflammatory cytokines, oxidant-antioxidant enzymes and histopathological parameters after Ngamma-nitro-L-arginine methyl ester and N-iminoethyl-L-ornithine administration. The study included 50 adult male Wistar albino rats (weighing 350 to 400 g). Rats were randomly allocated into four groups. Except in the control, moderate-level pulmonary contusion was created in all other groups. Intraperitoneal saline solution was performed in groups 1 and 2, 25 mg.kg-1 Ngamma-nitro-L-arginine methyl ester in group 3, and 20 mg.kg-1 N-iminoethyl-L-ornithine in group 4. Blood and lung tissues were studied biochemically and histopathologically. Best outcomes were recorded statistically significantly in groups with administration of Ngamma-nitro-L-arginine methyl ester and N-iminoethyl-L-ornithine when malondialdehyde response, mucous and histopathological values were examined. Significant improvement was detected in superoxide dismutase values in the group with administration of competitive nitric oxide synthase inhibitor Ngamma-nitro-L-arginine methyl ester. Nitric oxide values were substantially decreased in N-iminoethyl-L-ornithine group, while no significance was detected. Free oxygen radicals and lipid peroxidation played a role in pulmonary contusion after blunt lung trauma. According to biochemical and histopathological outcomes, effects of inflammation were decreased and protective effects were formed with administration of both Ngammanitro- L-arginine methyl ester and N-iminoethyl-L-ornithine.