Electrophoretic and chromatographic techniques for the differential diagnosis of a haemoglobin abnormality: Hb E heterozygosity

• Published in: Journal of chromatography Vol. 330; no. 2; p. 299
• Main Authors: Bosisio, A B, Rochette, J, Wajcman, H, Gianazza, E, Righetti, P G
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.1985
• Subjects: Chromatography, High Pressure Liquid; Hemoglobin A2 - analysis; Hemoglobin E - genetics; Hemoglobinopathies - diagnosis; Hemoglobins, Abnormal - genetics; Heterozygote; Humans; Hydrogen-Ion Concentration; Isoelectric Focusing; Thalassemia - blood

A method is described for separating haemoglobin (Hb) E (beta 26 Gly---Lys) from Hb A2 (a normal minor Hb component in adult blood). The technique allows the distinction between subjects carrying beta-thalassaemia trait and patients who are simultaneously alpha-thalassaemic and heterozygous for Hb E...