Mono- and bilayer smectic liquid crystal ordering in dense solutions of "gapped" DNA duplexes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 118; no. 12
• Main Authors: Gyawali, Prabesh, Saha, Rony, Smith, Gregory P, Salamonczyk, Miroslaw, Kharel, Prakash, Basu, Soumitra, Li, Ruipeng, Fukuto, Masafumi, Gleeson, James T, Clark, Noel A, Jákli, Antal, Balci, Hamza, Sprunt, Samuel
• Format: Journal Article
• Language: English
• Published: United States Proceedings of the National Academy of Sciences 23.03.2021; National Academy of Sciences
• Subjects: gapped DNA; MATERIALS SCIENCE; phase transitions; Physical Sciences; SAXS; smectic liquid crystals; smectic liquid crystal; phase transitions; “gapped” DNA; SAXS
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2019996118

Although its mesomorphic properties have been studied for many years, only recently has the molecule of life begun to reveal the true range of its rich liquid crystalline behavior. End-to-end interactions between concentrated, ultrashort DNA duplexes-driving the self-assembly of aggregates that organize into liquid crystal phases-and the incorporation of flexible single-stranded "gaps" in otherwise fully paired duplexes-producing clear evidence of an elementary lamellar (smectic-A) phase in DNA solutions-are two exciting developments that have opened avenues for discovery. Here, we report on a wider investigation of the nature and temperature dependence of smectic ordering in concentrated solutions of various "gapped" DNA (GDNA) constructs. We examine symmetric GDNA constructs consisting of two 48-base pair duplex segments bridged by a single-stranded sequence of 2 to 20 thymine bases. Two distinct smectic layer structures are observed for DNA concentration in the range [Formula: see text] mg/mL. One exhibits an interlayer periodicity comparable with two-duplex lengths ("bilayer" structure), and the other has a period similar to a single-duplex length ("monolayer" structure). The bilayer structure is observed for gap length ≳10 bases and melts into the cholesteric phase at a temperature between 30 °C and 35 °C. The monolayer structure predominates for gap length ≲10 bases and persists to [Formula: see text]C. We discuss models for the two layer structures and mechanisms for their stability. We also report results for asymmetric gapped constructs and for constructs with terminal overhangs, which further support the model layer structures.