Modulation of albumin-induced endoplasmic reticulum stress in renal proximal tubule cells by upregulation of mapk phosphatase-1

• Published in: Chemico-biological interactions Vol. 206; no. 1; pp. 47 - 54
• Main Authors: Gorostizaga, Alejandra, Mori Sequeiros García, Maria Mercedes, Acquier, Andrea, Gomez, Natalia V., Maloberti, Paula M., Mendez, Carlos F., Paz, Cristina
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 25.10.2013
• Subjects: adverse effects; Albumin; albumins; Animals; Cattle; Cells, Cultured; dephosphorylation; Didelphis - metabolism; Dual Specificity Phosphatase 1 - genetics; Dual Specificity Phosphatase 1 - metabolism; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Endoplasmic reticulum stress; ERK1/2; gene activation; kidney cells; Kidney Tubules, Proximal - cytology; Kidney Tubules, Proximal - metabolism; messenger RNA; mitogen-activated protein kinase; MKPs; OK cells; Oxidative Stress; phosphorylation; renal function; Reverse Transcriptase Polymerase Chain Reaction; Serum Albumin, Bovine - metabolism; stress response; transcription (genetics); Up-Regulation; urine; OK cells; MKPs; GRP78; Albumin; MAPKKs; ER; JNKs; SP600125; Endoplasmic reticulum stress; BSA; CHX; ERK1/2; MKP; Act D; ERKs; UPR; PD98059; SAPKs; OK; MAPK phosphatase; Anthra[1-9-cd]pyrazol-6(2H); c-Jun NH2-terminal protein kinases; MAPK kinases; actinomycin D; extracellular signal-regulated kinases; glucose-regulated protein 78; 2-amino-3-methoxyphenyl)4H-1-benzopyran-4-one; Free fatty acid albumin; cycloheximide; unfolded protein response; stress-activated protein kinases; Opossum kidney; endoplasmic reticulum

•Albumin rapidly increases both MKP-1 mRNA and protein levels.•The effect of albumin on MKP-1 mRNA levels is due to gene transcription activation.•Albumin induces MKP-1 protein accumulation promoting its stabilization.•Albumin induces GRP78 mRNA through an ERK-dependent mechanism.•MKP-1 induction do...