Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein

• Published in: Parasitology research (1987) Vol. 103; no. 5; pp. 1133 - 1140
• Main Authors: Ding, Jun, Bao, Weichao, Liu, Qun, Yu, Qi, Abdille, M. H, Wei, Zheng
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.10.2008; Springer-Verlag; Springer
• Subjects: Animals; antigens; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Chickens; Coccidiosis - immunology; Coccidiosis - veterinary; Dose-Response Relationship, Immunologic; Eimeria; Eimeria acervulina; Escherichia coli; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; immune response; immunization; Immunology; Invertebrates; Medical Microbiology; Microbiology; microtubules; oocysts; Original Paper; parasites; polymerization; Poultry Diseases - immunology; Poultry Diseases - parasitology; Protozoan Proteins - genetics; Protozoan Proteins - immunology; Protozoan Vaccines - immunology; Recombinant Proteins - immunology; subcutaneous injection; tubulin; Tubulin - genetics; Tubulin - immunology; Vaccines, Synthetic - immunology; weight gain; Duodenal Lesion; Sporulated Oocyst; Coccidiosis; Lymphocyte Proliferation Assay; Body Weight Gain; Immunoprotection; Protozoa; Apicomplexa; Vertebrata; Tubulin; Parasite; Eimeria acervulina; Chicken; Recombinant protein; Aves

Tubulins are heterodimeric molecules responsible for the polymerization of microtubules in apicomplexan parasites. The α-tubulin, a subcellular structural protein of Eimeria acervulina, was cloned and expressed in Escherichia coli as an α-tubulin-GST fusion protein. Immunogenicity of the recombinant...