Lactococcus lactis subsp. cremoris YRC3780 modifies function of mesenteric lymph node dendritic cells to modulate the balance of T cell differentiation inducing regulatory T cells

• Published in: Frontiers in immunology Vol. 15; p. 1395380
• Main Authors: Nakagawa, Ryogo, Gu, Wenting, Mizobuchi, Hibine, Kodera, Shuhei, Takano, Tomohiro, Wang, Yimei, Fujioka, Ikumi, Uchida, Kenji, Nakajima-Adachi, Haruyo, Hachimura, Satoshi
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 08.07.2024
• Subjects: allergy; Animals; Cell Differentiation - immunology; Coculture Techniques; Cytokines - metabolism; dendritic cells; Dendritic Cells - immunology; Female; Immunology; intestine; lactic acid bacteria; Lactococcus lactis - immunology; Lymph Nodes - immunology; Lymphocyte Activation - immunology; Mesentery - immunology; Mice; Mice, Inbred BALB C; Mice, Transgenic; regulatory T cells; T-Lymphocytes, Regulatory - immunology; regulatory T cells; allergy; dendritic cells; intestine; lactic acid bacteria
• DOI: 10.3389/fimmu.2024.1395380
• ISSN: 1664-3224

The intestinal immune system plays a pivotal role in the induction of immune responses against food. In the case of T cell response, dendritic cells (DCs) are especially important. However, the regulation of immune responses to food by intestinal DCs has been poorly described. In this study, we analyzed the effect of subsp. YRC3780, a lactic acid bacterial strain isolated from kefir, a traditional fermented milk product, on the immune responses induced by antigen presentation by intestinal DCs to T cells as well as the mechanism of action of these immunomodulatory effects. It has been shown that YRC3780 ameliorates the symptoms of pollinosis in both animal and human studies. CD11c cells from mesenteric lymph nodes (MLNs) of BALB/c mice were cultured as MLN DCs with YRC3780 and expression of genes inducing regulatory T cells (Tregs) was examined by qPCR. In addition, MLN DCs were cocultured with CD4 T cells from DO11.10 transgenic mice expressing an ovalbumin (OVA)-specific TCR and the OVA antigen peptide and YRC3780. Induction of Tregs was examined by flow cytometry, gene expression was analyzed by DNA microarray and qPCR, and the production of cytokines was measured by ELISA. MLN DCs from TLR2-deficient mice and components of YRC3780 were used to examine the recognition of YRC3780 by MLN DCs. YRC3780 enhanced the expression of genes involved in Treg induction in MLN DCs and induced Foxp3 CD4 T cells in an MLN DC and CD4 T-cell co-culture system. The effect on MLN DCs was likely mediated by receptors other than TLR2. Together with microarray analyses of CD4 T cell gene expression and cytokine ELISA, it was demonstrated that YRC3780 promoted the induction of Th1 and Tregs, and regulated the balance of Th1/Th2 and Treg/Th17 cells involving multiple genes via the antigen-presentation of MLN DCs. Our findings provide insights into the modulation of intestinal immune responses mediated by DCs and the antiallergic effects of lactic acid bacteria.