Evidence that small proteins translocate through silicon nitride pores in a folded conformation
The interaction of three proteins (histidine-containing phosphocarrier protein, HPr, calmodulin, CaM, and maltose binding protein, MBP) with synthetic silicon nitride (SiN(x)) membranes has been studied. The proteins which have a net negative charge were electrophoretically driven into pores of 7 an...
Saved in:
Published in | Journal of physics. Condensed matter Vol. 22; no. 45; p. 454133 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Bristol
IOP Publishing
17.11.2010
Institute of Physics |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The interaction of three proteins (histidine-containing phosphocarrier protein, HPr, calmodulin, CaM, and maltose binding protein, MBP) with synthetic silicon nitride (SiN(x)) membranes has been studied. The proteins which have a net negative charge were electrophoretically driven into pores of 7 and 5 nm diameter with a nominal length of 15 nm. The % blockade current and event duration were measured at three different voltages. For a translocation event it was expected that the % block would be constant with voltage whilst the event duration would decrease with increasing voltage. On the basis of these criteria, we deduce that MBP whose largest dimension is 6.5 nm does not translocate whereas up to 40% of CaM molecules can translocate the 7 nm pore as can a majority of HPr molecules, with some translocations being observed for the 5 nm pore. For translocation events the magnitude of the % blockade current is consistent with a folded conformation of the proteins surrounded by a hydration shell of 0.5-1.0 nm. |
---|---|
AbstractList | The interaction of three proteins (histidine-containing phosphocarrier protein, HPr, calmodulin, CaM, and maltose binding protein, MBP) with synthetic silicon nitride (SiN(x)) membranes has been studied. The proteins which have a net negative charge were electrophoretically driven into pores of 7 and 5 nm diameter with a nominal length of 15 nm. The % blockade current and event duration were measured at three different voltages. For a translocation event it was expected that the % block would be constant with voltage whilst the event duration would decrease with increasing voltage. On the basis of these criteria, we deduce that MBP whose largest dimension is 6.5 nm does not translocate whereas up to 40% of CaM molecules can translocate the 7 nm pore as can a majority of HPr molecules, with some translocations being observed for the 5 nm pore. For translocation events the magnitude of the % blockade current is consistent with a folded conformation of the proteins surrounded by a hydration shell of 0.5-1.0 nm. |
Author | Stefureac, Radu I Trivedi, Dhruti Lee, Jeremy S Marziali, Andre |
Author_xml | – sequence: 1 fullname: Stefureac, Radu I – sequence: 2 fullname: Trivedi, Dhruti – sequence: 3 fullname: Marziali, Andre – sequence: 4 fullname: Lee, Jeremy S |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23423757$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/21339619$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkE9rFDEYh4NU7Lb6FUou4mm6-TeZ5Cil2kLBSwveQiZ5x0ZmkjHJCn57s-y6PSgIgRx-zy_vm-cCncUUAaErSq4pUWpLdM87pZXYMrYVfTuCcv4KbSiXtJNCfT1DmxN0ji5K-U4IEYqLN-icNVZLqjfI3P4MHqIDXJ9txWWx84zXnCqEWHDNNpY5OVv3eU67b8-4hDm4FHEMNbcqXlOGgkPEFk9p9uBxS6eUF1tDim_R68nOBd4d70v09On28eaue_jy-f7m40PnBCO16wGkB0dGKx2MoLgeiCKD95q134KG3o_EK99LqcXEtB41UGb5MI5KS835JfpweLft_mMHpZolFAfzbCOkXTGq52LgjPaNlAfS5VRKhsmsOSw2_zKUmL1bs9dm9toMY0b05uC2Fa-OI3bjAv5U-yOzAe-PgC3OzlOT50J54bhgfOiHxnUHLqT1lP57qFn91Hj6N_-fZX8DDDShOg |
CODEN | JCOMEL |
CitedBy_id | crossref_primary_10_1021_acs_jpcc_2c01459 crossref_primary_10_1021_acs_nanolett_5b02309 crossref_primary_10_1007_s00216_020_02717_2 crossref_primary_10_4161_pri_18665 crossref_primary_10_1038_s41598_017_06435_1 crossref_primary_10_1021_acs_analchem_4c01700 crossref_primary_10_1002_smll_201601157 crossref_primary_10_1021_acs_analchem_7b01550 crossref_primary_10_3390_ijms21082808 crossref_primary_10_1007_s41664_019_00092_1 crossref_primary_10_1039_c2nr30951c crossref_primary_10_1098_rstb_2016_0230 crossref_primary_10_1038_s41467_017_01006_4 |
Cites_doi | 10.1021/bi015784n 10.1063/1.1684724 10.1021/ac7025207 10.1021/ja901088b 10.1093/oxfordjournals.jbchem.a122243 10.1021/nl100861c 10.1088/0957-4484/18/30/305505 10.1038/35084037 10.1063/1.2767206 10.1073/pnas.0709207104 10.1073/pnas.93.24.13770 10.1038/nbt.1495 10.1063/1.2180868 10.1039/b813796j 10.1016/j.chroma.2008.04.029 10.1021/ja0739943 10.1016/S0021-9258(18)51515-5 10.1038/nnano.2007.27 10.1016/S0021-9258(18)41556-6 10.1038/nsb0995-768 |
ContentType | Journal Article |
Copyright | 2015 INIST-CNRS |
Copyright_xml | – notice: 2015 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1088/0953-8984/22/45/454133 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Physics |
EISSN | 1361-648X |
EndPage | 454133 |
ExternalDocumentID | 10_1088_0953_8984_22_45_454133 21339619 23423757 |
Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GrantInformation_xml | – fundername: NHGRI NIH HHS grantid: R01HG003248 |
GroupedDBID | - 02 02O 1JI 1PV 1WK 4.4 53G 5B3 5GY 5PX 5VS 5ZH 5ZI 7.M 7.Q 8RP 9BW AAGCD AAGID AAJIO AALHV ABHWH ABQJV ACGFS ACNCT ADIYS AEFHF AFYNE AHSEE ALMA_UNASSIGNED_HOLDINGS ASPBG ATQHT AVWKF AZFZN BBWZM CJUJL CS3 EBS EDWGO EJD EMSAF EPQRW EQZZN F5P FEDTE HAK HVGLF IHE IOP IZVLO KNG KOT LAP M45 MGA N5L N9A NT- NT. P2P Q02 RIN RNS RO9 ROL RPA RW3 S3P SY9 TN5 UNR UQL W28 WH7 X XFK XPP ZMT --- -~X 08R 29L 6TJ 8WZ A6W AAGCF AAJKP AATNI AAYJJ ABCXL ABLJU ABVAM ACAFW ACHIP AFFNX AI. AKPSB CBCFC CEBXE CRLBU H~9 IJHAN IQODW JCGBZ KC5 MVM PJBAE R4D RKQ T37 VH1 XOL YQT ~02 AERVB AOAED CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c420t-5ee6dec0ba6cebe83970807dd92088e9e5db0d8d56694f299b9e12a37bb896933 |
IEDL.DBID | IOP |
ISSN | 0953-8984 |
IngestDate | Fri Aug 16 21:53:40 EDT 2024 Thu Sep 26 19:29:32 EDT 2024 Sat Sep 28 08:48:29 EDT 2024 Sun Oct 22 16:07:42 EDT 2023 Tue Nov 10 14:14:40 EST 2020 Mon May 13 16:01:46 EDT 2019 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 45 |
Keywords | Translocation Electrophoresis Nanopore Hydration Protein |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c420t-5ee6dec0ba6cebe83970807dd92088e9e5db0d8d56694f299b9e12a37bb896933 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 21339619 |
PQID | 853473215 |
PQPubID | 23479 |
PageCount | 1 |
ParticipantIDs | crossref_primary_10_1088_0953_8984_22_45_454133 pubmed_primary_21339619 pascalfrancis_primary_23423757 iop_primary_10_1088_0953_8984_22_45_454133 proquest_miscellaneous_853473215 |
PublicationCentury | 2000 |
PublicationDate | 2010-11-17 |
PublicationDateYYYYMMDD | 2010-11-17 |
PublicationDate_xml | – month: 11 year: 2010 text: 2010-11-17 day: 17 |
PublicationDecade | 2010 |
PublicationPlace | Bristol |
PublicationPlace_xml | – name: Bristol – name: England |
PublicationTitle | Journal of physics. Condensed matter |
PublicationTitleAlternate | J Phys Condens Matter |
PublicationYear | 2010 |
Publisher | IOP Publishing Institute of Physics |
Publisher_xml | – name: IOP Publishing – name: Institute of Physics |
References | 11 13 14 Jia Z (19) 1993; 268 17 18 1 2 3 4 5 6 Delbaere L T J (16) 1989; 264 Coulter W H (20) 1953 7 Kurokawa H (15) 1988; 103 8 9 Tabard-Cossa V (12) 2007; 18 10 21 |
References_xml | – ident: 17 doi: 10.1021/bi015784n – ident: 13 doi: 10.1063/1.1684724 – ident: 8 doi: 10.1021/ac7025207 – ident: 11 doi: 10.1021/ja901088b – volume: 103 start-page: 8 issn: 0021-924X year: 1988 ident: 15 publication-title: J. Biochem. doi: 10.1093/oxfordjournals.jbchem.a122243 contributor: fullname: Kurokawa H – ident: 6 doi: 10.1021/nl100861c – volume: 18 start-page: 305505 issn: 0957-4484 year: 2007 ident: 12 publication-title: Nanotechnology doi: 10.1088/0957-4484/18/30/305505 contributor: fullname: Tabard-Cossa V – ident: 5 doi: 10.1038/35084037 – ident: 7 doi: 10.1063/1.2767206 – ident: 21 doi: 10.1073/pnas.0709207104 – ident: 1 doi: 10.1073/pnas.93.24.13770 – ident: 2 doi: 10.1038/nbt.1495 – ident: 9 doi: 10.1063/1.2180868 – ident: 3 doi: 10.1039/b813796j – ident: 14 doi: 10.1016/j.chroma.2008.04.029 – ident: 10 doi: 10.1021/ja0739943 – volume: 264 start-page: 18645 year: 1989 ident: 16 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)51515-5 contributor: fullname: Delbaere L T J – ident: 4 doi: 10.1038/nnano.2007.27 – volume: 268 start-page: 22490 year: 1993 ident: 19 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)41556-6 contributor: fullname: Jia Z – year: 1953 ident: 20 contributor: fullname: Coulter W H – ident: 18 doi: 10.1038/nsb0995-768 |
SSID | ssj0004834 |
Score | 2.1551952 |
Snippet | The interaction of three proteins (histidine-containing phosphocarrier protein, HPr, calmodulin, CaM, and maltose binding protein, MBP) with synthetic silicon... |
SourceID | proquest crossref pubmed pascalfrancis iop |
SourceType | Aggregation Database Index Database Enrichment Source Publisher |
StartPage | 454133 |
SubjectTerms | Analytical, structural and metabolic biochemistry Biological and medical sciences Diffusion Fundamental and applied biological sciences. Psychology General aspects, investigation methods Motion Nanostructures - chemistry Nanostructures - ultrastructure Particle Size Porosity Protein Conformation Protein Folding Proteins Proteins - chemistry Silicon Compounds - chemistry |
Title | Evidence that small proteins translocate through silicon nitride pores in a folded conformation |
URI | http://iopscience.iop.org/0953-8984/22/45/454133 https://www.ncbi.nlm.nih.gov/pubmed/21339619 https://search.proquest.com/docview/853473215 |
Volume | 22 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LS8QwEB5UELz4ftQXOYgHobtrmrTNUUQRwRcoeAtJk-Li2l223Yu_3km6XV1cUaGHQjPTdmaS-ULmAXCU5lQYzk5DqjluUGJBQ6UFC41geW5PaUcrl418cxtfPbHrZ_48B00TxG5_MF75W3jrT_JdQbQwFSlrU9pmHC9cd115T1fmxGXs3d1_JkKm_hh5QtKkBOMubzabKW80j690sZGqRPHkdV-Ln4Gnd0CXK_DQpPHUcSevrVGlW9n796qOf_63VVgeo1FyVpvPGszZYh0WfVRoVm6AbHqOkupFVaR8U70e8ZUdukVJKufmnC-s3HPf7oeU3R6aVkFwoRgiKUF0b0vSLYgieb9nrCH4dJIwuQlPlxeP51fhuCNDmDHaqUJubWxshgqMM9Q-gqsEEWdijKAoYyssN7pjUoMYEXWNnk4L1LeKEq1TEYso2oKFol_YHSBCJBqtgecqzxgyUIJxrjnu1DuxjjQLoN1oRg7qwhvSH5inqXRik05sklLJuKzFFsAxynUyePYgOTB5ACdfB_7G9XDKICZk1NVOTHgSAGksROLUdOctqrD9USkRCbEkQkwVwHZtOZ_EyFng3nX3P5-yB0s-dsEFISb7sFANR_YAIVGlD_00-AASCP0j |
link.rule.ids | 315,786,790,1564,27957,27958,53941 |
linkProvider | IOP Publishing |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9wwEB7BVq240ActhNKtD1UPSNksjp3YRwSseLSUQ5G4WXbsqCuW7IpkL_x6xs5uKBWoqirlEMkeyx6PPd_I8wD4IkoqLWd7MTUcDZRM0lgbyWIrWVm6PTo02kcjfz_Pji_Z6RW_WoGjLhZmOltc_QP8bRMFtyxcOMSJxGdIi4UULKE0YRw_vIjTZGbLVXjBU8n8-Tz5cfEQHinC43JHtwwUfnasRzpqFefhPSZ1jUwr22oXz8PRoJZGr1v3kTpkM_TeKNeDeWMGxd0fuR7_e8VvYH0BXMl-S_QWVlz1Dl4GB9Ki3gC1LE9Kml-6IfWNnkxISAIxrmrSeI3o1Wbj20NlIFKPJyiFFcE75RZJCRoCribjimhSTifWWYKtXWzle7gcHf08OI4XxRvigtFhE3PnMusK3OusQEFBHJYjOM2tlRSX5KTj1gytsAgnUSxQKRqJoqHT3BghM5mmH6BXTSu3BUTK3KDg8FKXBcMBtGScG45G_TAzqWERJMvtUrM2R4cKb-tCKM865VmnKFWMq5Z1EXxFXnedn-6kkL8R7P7e8W-j9h9JSUdGfZrFnOcRkKXYKDzF_mlGV246rxWCJpanCL8i2GzF6YEYR5Zo5m7_y1Q-w6uLw5H6dnJ-9hHWgseDd13Md6DX3M7dJwRSjemHY3IPwrMNHQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Evidence+that+small+proteins+translocate+through+silicon+nitride+pores+in+a+folded+conformation&rft.jtitle=Journal+of+physics.+Condensed+matter&rft.au=Stefureac%2C+Radu+I&rft.au=Trivedi%2C+Dhruti&rft.au=Marziali%2C+Andre&rft.au=Lee%2C+Jeremy+S&rft.date=2010-11-17&rft.pub=IOP+Publishing&rft.issn=0953-8984&rft.eissn=1361-648X&rft.volume=22&rft.spage=454133&rft_id=info:doi/10.1088%2F0953-8984%2F22%2F45%2F454133&rft.externalDBID=n%2Fa&rft.externalDocID=10_1088_0953_8984_22_45_454133 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0953-8984&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0953-8984&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0953-8984&client=summon |