A five-minute DNA extraction method for expedited detection of Phytophthora ramorum following prescreening using Phytophthora spp. lateral flow devices

• Published in: Journal of microbiological methods Vol. 81; no. 2; pp. 116 - 120
• Main Authors: Tomlinson, J.A., Dickinson, M., Hobden, E., Robinson, S., Giltrap, P.M., Boonham, N.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2010; Elsevier
• Subjects: Biological and medical sciences; Diagnostic sensitivity; Diagnostic specificity; DNA - isolation & purification; DNA isolation; Filtration - methods; Fundamental and applied biological sciences. Psychology; Microbiological Techniques - methods; Microbiology; Phytophthora; Phytophthora - genetics; Phytophthora - isolation & purification; Plant extracts; Polymerase Chain Reaction; Real-time PCR; Sensitivity and Specificity; Sudden oak death; Time Factors; Diagnostic sensitivity; Sudden oak death; Diagnostic specificity; Real-time PCR; DNA isolation; Cardiocirculatory arrest; Sudden death; Cardiovascular disease; Method; Real time; Polymerase chain reaction; Sensitivity; Specificity; Isolation; Detection

In a direct comparison with established methods for Phytophthora ramorum detection (isolation followed by morphological identification, or conventional DNA extraction followed by TaqMan real-time PCR) a rapid, simplified detection method in which membranes of lateral flow devices (LFDs) are added di...